Not CRISPR data: the image illustrates antibody quality for visualizing CRISPR experiment results.
Not CRISPR data: the image illustrates antibody quality for visualizing CRISPR experiment results.
SLMAP CRISPR/Cas9 KO Plasmid (m2): sc-429965-KO-2
- Target species: mouse
- 20 µg of transfection-ready, purified plasmid DNA; Suitable for up to 20 transfections
- SLMAP CRISPR/Cas9 Knockout (KO) Plasmid (m2) is a pool of plasmids, each encoding Cas9 nuclease and a target-specific 20 nt guide RNA (gRNA) designed for maximum knockout efficiency using sequences derived from the GeCKO v2 library
- gRNA sequences direct Cas9 to induce site-specific double-strand breaks (DSBs) in the SLMAP genomic locus, resulting in gene knockout through non-homologous end joining (NHEJ)
- The puromycin resistance and RFP genes are flanked by LoxP sites, enabling removal of selection markers via Cre recombinase (Cre Vector: sc-418923) after establishing stable knockout cell lines
- Following transfection, gene knockout efficiency can be assayed by WB, IF or IHC using antibody: SLMAP Antibody (B-9): sc-393336
Ordering Information
| Product Name | Catalog # | UNIT | Price | Qty | FAVORITES | |
SLMAP CRISPR/Cas9 KO Plasmid (m2) | sc-429965-KO-2 | 20 µg | $397.00 |
Overview
Slmap encodes sarcolemma-associated protein (SLMAP), a tail-anchored membrane protein enriched at intracellular membranes including the sarcolemma, endoplasmic reticulum, and nuclear envelope. SLMAP participates in membrane organization and trafficking, junctional signaling, and cytoskeletal coupling, and has been implicated in regulation of centrosome function and cell-cycle progression. In excitable tissues, SLMAP is linked to ion channel and calcium-handling microdomains that support electrical activity and contractile homeostasis. Altered SLMAP expression or localization has been associated with cardiac conduction phenotypes and cardiomyopathy-related mechanisms, making it relevant for studies of membrane architecture and electrophysiology.
SLMAP CRISPR/Cas9 KO Plasmid (m2) is a pool of plasmids designed for targeted disruption of the Slmap gene in mouse cell lines. Each plasmid co-expresses a unique single guide RNA (sgRNA) targeting a distinct site within the Slmap together with the Streptococcus pyogenes Cas9 nuclease. The plasmids also encode GFP, allowing fluorescent identification and enrichment of successfully transfected cells by fluorescence microscopy or flow cytometry.
The multi-guide design increases the likelihood of generating insertions or deletions (indels) that disrupt the Slmap open reading frame following Cas9-mediated double-strand break formation. DNA breaks introduced by the CRISPR/Cas9 system are repaired through endogenous non-homologous end joining (NHEJ) pathways, frequently resulting in frameshift mutations that abolish SLMAP protein expression.
This CRISPR knockout system enables efficient generation of Slmap-deficient cell models for investigation of SLMAP signaling, functional genomics studies, cancer biology research, and evaluation of therapeutic responses in human cell lines.
Key Features
- sgRNAs targeting Slmap exon(s) critical for SLMAP function
- Co-expression of SpCas9 and sgRNA from a single plasmid for simplified delivery
- GFP reporter for identification of transfected cells
- Pool of plasmids targeting multiple Slmap genomic sites to improve knockout efficiency
- Compatible with delivery by transfection
Design Variants
CRISPRs +/- HDRs
- gRNAs encoded by SLMAP CRISPR/Cas9 KO Plasmid (m) and SLMAP CRISPR/Cas9 KO Plasmid (m2) target distinct sites within the Slmap locus. One or both targeting designs may be available. See Related Products for availability.
- HDR donor constructs encoded by SLMAP HDR Plasmid (m) and SLMAP HDR Plasmid (m2) contain a puromycin resistance cassette and an RFP reporter flanked by Slmap homology arms to support homology-directed repair at defined Slmap target sites corresponding to the CRISPR/Cas9 KO designs. HDR donor availability may vary. See Related Products for availability.
For Research Use Only. Not Intended for Diagnostic or Therapeutic Use.