Slit2 Antibody (F-7): sc-514499

Slit2 Antibody (F-7) is a mouse monoclonal IgM antibody that detects Slit2 of mouse, rat, and human origin by western blotting (WB), immunoprecipitation (IP), immunofluorescence (IF), and enzyme-linked immunosorbent assay (ELISA). Anti-Slit2 antibody (F-7) is available as the non-conjugated form. Slit2 plays a crucial role in the development of the nervous system, particularly in axonal guidance, by serving as a signaling ligand for the Roundabout (Robo) receptor, which is essential for directing the growth of neuronal axons. Slit2 is predominantly expressed in the midline of the central nervous system during embryonic development, where Slit2 regulates the movement of mesodermal cells during gastrulation, a critical phase in early development. The interaction between Slit2 and Robo receptors is vital for establishing proper neural pathways, and disruptions in this signaling can lead to developmental disorders and neurodegenerative diseases. Anti-Slit2 antibody (F-7) enables researchers to gain insights into the mechanisms of neural development and the potential implications of Slit2 in pathologies associated with aberrant axonal guidance.

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Slit2 Antibody (F-7) References:

1. Slit proteins bind Robo receptors and have an evolutionarily conserved role in repulsive axon guidance.  |  Brose, K., et al. 1999. Cell. 96: 795-806. PMID: 10102268
2. Distinct but overlapping expression patterns of two vertebrate slit homologs implies functional roles in CNS development and organogenesis.  |  Holmes, GP., et al. 1998. Mech Dev. 79: 57-72. PMID: 10349621
3. The mouse SLIT family: secreted ligands for ROBO expressed in patterns that suggest a role in morphogenesis and axon guidance.  |  Yuan, W., et al. 1999. Dev Biol. 212: 290-306. PMID: 10433822
4. Chemorepulsion of neuronal migration by Slit2 in the developing mammalian forebrain.  |  Hu, H. 1999. Neuron. 23: 703-11. PMID: 10482237
5. Slit2 is a repellent for retinal ganglion cell axons.  |  Niclou, SP., et al. 2000. J Neurosci. 20: 4962-74. PMID: 10864954
6. Retinal ganglion cell axon guidance in the mouse optic chiasm: expression and function of robos and slits.  |  Erskine, L., et al. 2000. J Neurosci. 20: 4975-82. PMID: 10864955
7. slit: an extracellular protein necessary for development of midline glia and commissural axon pathways contains both EGF and LRR domains.  |  Rothberg, JM., et al. 1990. Genes Dev. 4: 2169-87. PMID: 2176636
8. Activation of Slit2-Robo1 signaling promotes liver fibrosis.  |  Chang, J., et al. 2015. J Hepatol. 63: 1413-20. PMID: 26264936
9. Tumoural activation of TLR3-SLIT2 axis in endothelium drives metastasis.  |  Tavora, B., et al. 2020. Nature. 586: 299-304. PMID: 32999457
10. SLIT2/ROBO signaling in tumor-associated microglia and macrophages drives glioblastoma immunosuppression and vascular dysmorphia.  |  Geraldo, LH., et al. 2021. J Clin Invest. 131: PMID: 34181595
11. Coadaptation fostered by the SLIT2-ROBO1 axis facilitates liver metastasis of pancreatic ductal adenocarcinoma.  |  Li, Q., et al. 2023. Nat Commun. 14: 861. PMID: 36792623
12. Cancer associated fibroblast derived SLIT2 drives gastric cancer cell metastasis by activating NEK9.  |  Lu, G., et al. 2023. Cell Death Dis. 14: 421. PMID: 37443302

Monoclonal antibodies for mammalian and non-mammalian protein targets represent essentially all targets covered by polyclonal antibodies. Over 8,300 of our antibodies are offered as ImmunoCruz^® conjugates which eliminates the need for a secondary antibody.

Santa Cruz Biotechnology antibodies have over 360,000 research citations. Anti-Slit2 Antibody (F-7) has 3 citations in a variety of scientific publications.

Primary antibodies like Anti-Slit2 Antibody (F-7) for mammalian target proteins are recommended for the detection of a range of mammalian species, primarily of mouse, rat and human species. Many mammalian antibodies are also ideal for veterinary research and reactive with ovine, porcine, caprine, feline, canine, bovine and equine protein targets. Santa Cruz Biotechnology primary monoclonal antibodies are suitable for laboratory research applications and are not intended for diagnostic or therapeutic use.

Slit2 Antibody (F-7) Specifications

Alternate Product Name: Slit2

Product Clone Name: F-7

Classification: Primary Antibody

Clonality: Monoclonal Antibody

Antibody Isotype: IgM kappa light chain

Amino Acids: 1441-1464

Epitope Target: Slit2

Epitope Species: human

Product Reactivity/Detection: Slit2

Species Reactivity/Detection: mouse, rat, human

Additional Mammalian Reactivity: N/A

Additional Non-Mammalian Reactivity: N/A

Applications: WB, IP, IF, ELISA

Vialing Information: Each vial contains 200 µg IgM kappa light chain in 1.0 ml of PBS with < 0.1% sodium azide and 0.1% gelatin

Research Citations: 3 citations in various scientific publications

Available Conjugates/Forms for Slit2 Antibody (F-7): P: Blocking Peptide