SLC25A36 CRISPR/Cas9 KO Plasmid (h): sc-412475
- Target species: human
- 20 µg of transfection-ready, purified plasmid DNA; Suitable for up to 20 transfections
- SLC25A36 CRISPR/Cas9 Knockout (KO) Plasmid (h) is a pool of plasmids, each encoding Cas9 nuclease and a target-specific 20 nt guide RNA (gRNA) designed for maximum knockout efficiency using sequences derived from the GeCKO v2 library
- gRNA sequences direct Cas9 to induce site-specific double-strand breaks (DSBs) in the SLC25A36 genomic locus, resulting in gene knockout through non-homologous end joining (NHEJ)
- The puromycin resistance and RFP genes are flanked by LoxP sites, enabling removal of selection markers via Cre recombinase (Cre Vector: sc-418923) after establishing stable knockout cell lines
Ordering Information
| Product Name | Catalog # | UNIT | Price | Qty | FAVORITES | |
SLC25A36 CRISPR/Cas9 KO Plasmid (h) | sc-412475 | 20 µg | $397.00 |
Overview
SLC25A36 encodes a mitochondrial inner membrane solute carrier that functions as a nucleotide transporter, supporting exchange of pyrimidine nucleotides between the cytosol and mitochondrial matrix. By sustaining intramitochondrial dNTP availability, SLC25A36 contributes to mitochondrial DNA replication and repair, oxidative phosphorylation capacity, and broader mitochondrial homeostasis. Perturbation of nucleotide transport can couple mitochondrial dysfunction to altered energy metabolism, redox balance, and replication stress, processes frequently investigated in neurodegeneration, inflammatory signaling, and tumor biology. As a mitochondrial carrier family member, SLC25A36 is commonly studied in the context of organelle biogenesis, stress responses, and metabolic adaptation.
SLC25A36 CRISPR/Cas9 KO Plasmid (h) is a pool of plasmids designed for targeted disruption of the SLC25A36 gene in human cell lines. Each plasmid co-expresses a unique single guide RNA (sgRNA) targeting a distinct site within the SLC25A36 together with the Streptococcus pyogenes Cas9 nuclease. The plasmids also encode GFP, allowing fluorescent identification and enrichment of successfully transfected cells by fluorescence microscopy or flow cytometry.
The multi-guide design increases the likelihood of generating insertions or deletions (indels) that disrupt the SLC25A36 open reading frame following Cas9-mediated double-strand break formation. DNA breaks introduced by the CRISPR/Cas9 system are repaired through endogenous non-homologous end joining (NHEJ) pathways, frequently resulting in frameshift mutations that abolish SLC25A36 protein expression.
This CRISPR knockout system enables efficient generation of SLC25A36-deficient cell models for investigation of SLC25A36 signaling, functional genomics studies, cancer biology research, and evaluation of therapeutic responses in human cell lines.
Key Features
- sgRNAs targeting SLC25A36 exon(s) critical for SLC25A36 function
- Co-expression of SpCas9 and sgRNA from a single plasmid for simplified delivery
- GFP reporter for identification of transfected cells
- Pool of plasmids targeting multiple SLC25A36 genomic sites to improve knockout efficiency
- Compatible with delivery by transfection
Design Variants
CRISPRs +/- HDRs
- gRNAs encoded by SLC25A36 CRISPR/Cas9 KO Plasmid (h) and SLC25A36 CRISPR/Cas9 KO Plasmid (h2) target distinct sites within the SLC25A36 locus. One or both targeting designs may be available. See Related Products for availability.
- HDR donor constructs encoded by SLC25A36 HDR Plasmid (h) and SLC25A36 HDR Plasmid (h2) contain a puromycin resistance cassette and an RFP reporter flanked by SLC25A36 homology arms to support homology-directed repair at defined SLC25A36 target sites corresponding to the CRISPR/Cas9 KO designs. HDR donor availability may vary. See Related Products for availability.
For Research Use Only. Not Intended for Diagnostic or Therapeutic Use.