SLBP Antibody (XX-3): sc-101140

SLBP Antibody (XX-3) is a mouse monoclonal IgG2a antibody that detects SLBP in human samples through applications such as western blotting (WB), immunoprecipitation (IP), immunofluorescence (IF), and enzyme-linked immunosorbent assay (ELISA). SLBP, or stem-loop binding protein, plays a crucial role in the regulation of histone mRNA, which is essential for proper cell cycle progression and gene expression. Unlike typical mRNAs, replication-dependent histone mRNAs lack polyadenylated tails and instead terminate with a conserved stem-loop structure, which is specifically recognized by SLBP. This interaction is vital as SLBP facilitates the processing of histone pre-mRNA by binding to U7 small nuclear ribonucleoprotein (SnRNP), thereby ensuring the correct maturation of histone mRNA necessary for histone protein synthesis during the cell cycle. The dynamic regulation of SLBP levels, particularly its increase in late G1 and decrease at the S/G2 transition, is critical for maintaining the balance of histone production in response to cellular needs. Additionally, SLBP undergoes post-translational modifications, including phosphorylation at threonine 99 and threonine 104, which are pivotal for its degradation in late S phase cells, highlighting the importance of these modifications in controlling SLBP activity and stability. With its ability to form stable complexes in both the nucleus and cytoplasm, anti-SLBP antibody (XX-3) is an invaluable tool for researchers studying histone mRNA regulation and its implications in cellular processes.

The preferred SLBP antibody is SLBP Antibody (H-3): sc-376310.