SLBP Antibody (H-3): sc-376310
- SLBP Antibody (H-3) is a mouse monoclonal IgG1 κ provided at 200 µg/ml
- raised against amino acids 56-215 mapping within an internal region of SLBP of human origin
- Anti-SLBP Antibody (H-3) is recommended for detection of SLBP of mouse, rat and human origin by WB, IP, IF, IHC(P) and ELISA
- Anti-SLBP Antibody (H-3) is available conjugated to agarose for IP; HRP for WB, IHC(P) and ELISA; and to either phycoerythrin or FITC for IF, IHC(P) and FCM
- also available conjugated to Alexa Fluor® 488, Alexa Fluor® 546, Alexa Fluor® 594 or Alexa Fluor® 647 for WB (RGB), IF, IHC(P) and FCM, and for use with RGB fluorescent imaging systems, such as iBright™ FL1000, FluorChem™, Typhoon, Azure and other comparable systems
- also available conjugated to Alexa Fluor® 680 or Alexa Fluor® 790 for WB (NIR), IF and FCM; for use with Near-Infrared (NIR) detection systems, such as LI-COR®Odyssey®, iBright™ FL1000, FluorChem™, Typhoon, Azure and other comparable systems
- TransCruz reagent for ChIP application (sc-376310 X, 200 µg/0.1 ml)
- m-IgG1 BP-HRP and m-IgGκ BP-HRP are the preferred secondary detection reagents for SLBP Antibody (H-3) for WB and IHC(P) applications. These reagents are now offered in bundles with SLBP Antibody (H-3) (see ordering information below).
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SEE ALSO...
SLBP Antibody (H-3) is a mouse monoclonal IgG1 kappa light chain antibody that detects SLBP in mouse, rat, and human samples through applications such as western blotting (WB), immunoprecipitation (IP), immunofluorescence (IF), immunohistochemistry, and enzyme-linked immunosorbent assay (ELISA). Anti-SLBP antibody (H-3) is available in both non-conjugated and various conjugated forms, including agarose, horseradish peroxidase (HRP), phycoerythrin (PE), fluorescein isothiocyanate (FITC), and multiple Alexa Fluor® conjugates. The stem-loop binding protein (SLBP) plays a crucial role in regulating histone mRNA, which is essential for proper cell cycle progression and gene expression. Unlike typical mRNAs, replication-dependent histone mRNAs lack polyadenylated tails and instead terminate with a conserved stem-loop structure. SLBP binds to this stem-loop at the 3′ end of histone mRNA, facilitating interaction with U7 small nuclear ribonucleoprotein (SnRNP) to promote 3′ end processing, which is vital for stability and translation of these mRNAs. SLBP forms a stable complex with U7 SnRNP in both nucleus and cytoplasm, and SLBP levels fluctuate throughout the cell cycle, increasing during late G1 and decreasing at the S/G2 transition. SLBP regulation is primarily post-translational, with specific phosphorylation events on threonine 99 and threonine 104 leading to degradation in late S phase cells. hZFP100, a zinc finger protein, interacts specifically with the SLBP/RNA complex, highlighting the intricate network of protein interactions that govern histone mRNA metabolism.
Alexa Fluor® is a trademark of Molecular Probes Inc., OR., USA
LI-COR® and Odyssey® are registered trademarks of LI-COR Biosciences
SLBP Antibody (H-3) References:
- Stem-loop binding protein facilitates 3'-end formation by stabilizing U7 snRNP binding to histone pre-mRNA. | Dominski, Z., et al. 1999. Mol Cell Biol. 19: 3561-70. PMID: 10207079
- Stem-loop binding protein, the protein that binds the 3' end of histone mRNA, is cell cycle regulated by both translational and posttranslational mechanisms. | Whitfield, ML., et al. 2000. Mol Cell Biol. 20: 4188-98. PMID: 10825184
- A novel zinc finger protein is associated with U7 snRNP and interacts with the stem-loop binding protein in the histone pre-mRNP to stimulate 3'-end processing. | Dominski, Z., et al. 2002. Genes Dev. 16: 58-71. PMID: 11782445
- The histone 3'-terminal stem-loop-binding protein enhances translation through a functional and physical interaction with eukaryotic initiation factor 4G (eIF4G) and eIF3. | Ling, J., et al. 2002. Mol Cell Biol. 22: 7853-67. PMID: 12391154
- Phosphorylation of stem-loop binding protein (SLBP) on two threonines triggers degradation of SLBP, the sole cell cycle-regulated factor required for regulation of histone mRNA processing, at the end of S phase. | Zheng, L., et al. 2003. Mol Cell Biol. 23: 1590-601. PMID: 12588979
- The protein that binds the 3' end of histone mRNA: a novel RNA-binding protein required for histone pre-mRNA processing. | Wang, ZF., et al. 1996. Genes Dev. 10: 3028-40. PMID: 8957003
- The gene for histone RNA hairpin binding protein is located on human chromosome 4 and encodes a novel type of RNA binding protein. | Martin, F., et al. 1997. EMBO J. 16: 769-78. PMID: 9049306
Ordering Information
| Product Name | Catalog # | UNIT | Price | Qty | FAVORITES | |
SLBP Antibody (H-3) | sc-376310 | 200 µg/ml | $322.00 | |||
SLBP Antibody (H-3): m-IgGκ BP-HRP Bundle | sc-522912 | 200 µg Ab, 40 µg BP | $361.00 | |||
SLBP Antibody (H-3): m-IgG1 BP-HRP Bundle | sc-533536 | 200 µg Ab; 20 µg BP | $361.00 | |||
SLBP Antibody (H-3) X | sc-376310 X | 200 µg/0.1 ml | $322.00 | |||
SLBP Antibody (H-3) AC | sc-376310 AC | 500 µg/ml, 25% agarose | $424.00 | |||
SLBP Antibody (H-3) HRP | sc-376310 HRP | 200 µg/ml | $322.00 | |||
SLBP Antibody (H-3) FITC | sc-376310 FITC | 200 µg/ml | $336.00 | |||
SLBP Antibody (H-3) PE | sc-376310 PE | 200 µg/ml | $349.00 | |||
SLBP Antibody (H-3) Alexa Fluor® 488 | sc-376310 AF488 | 200 µg/ml | $364.00 | |||
SLBP Antibody (H-3) Alexa Fluor® 546 | sc-376310 AF546 | 200 µg/ml | $364.00 | |||
SLBP Antibody (H-3) Alexa Fluor® 594 | sc-376310 AF594 | 200 µg/ml | $364.00 | |||
SLBP Antibody (H-3) Alexa Fluor® 647 | sc-376310 AF647 | 200 µg/ml | $364.00 | |||
SLBP Antibody (H-3) Alexa Fluor® 680 | sc-376310 AF680 | 200 µg/ml | $364.00 | |||
SLBP Antibody (H-3) Alexa Fluor® 790 | sc-376310 AF790 | 200 µg/ml | $364.00 |