SLAIN2 CRISPR/Cas9 KO Plasmid (m): sc-429242
- Target species: mouse
- 20 µg of transfection-ready, purified plasmid DNA; Suitable for up to 20 transfections
- SLAIN2 CRISPR/Cas9 Knockout (KO) Plasmid (m) is a pool of plasmids, each encoding Cas9 nuclease and a target-specific 20 nt guide RNA (gRNA) designed for maximum knockout efficiency using sequences derived from the GeCKO v2 library
- gRNA sequences direct Cas9 to induce site-specific double-strand breaks (DSBs) in the SLAIN2 genomic locus, resulting in gene knockout through non-homologous end joining (NHEJ)
- The puromycin resistance and RFP genes are flanked by LoxP sites, enabling removal of selection markers via Cre recombinase (Cre Vector: sc-418923) after establishing stable knockout cell lines
Ordering Information
| Product Name | Catalog # | UNIT | Price | Qty | FAVORITES | |
SLAIN2 CRISPR/Cas9 KO Plasmid (m) | sc-429242 | 20 µg | $397.00 |
Overview
Slain2 encodes SLAIN2, a microtubule plus-end–tracking regulator that cooperates with EB proteins and other +TIP complexes to promote persistent microtubule growth, cortical capture, and proper spindle dynamics. Through its role in organizing microtubule–dependent trafficking and cell polarity, SLAIN2 supports processes such as mitotic progression, intracellular transport, and neurite outgrowth. Perturbation of +TIP networks and microtubule stability is broadly relevant to neurodevelopmental and neurodegenerative phenotypes as well as chromosomal instability in proliferative cell states. Mouse Slain2 therefore serves as a tractable model for interrogating cytoskeletal control of cell division and differentiation in mammalian systems.
SLAIN2 CRISPR/Cas9 KO Plasmid (m) is a pool of plasmids designed for targeted disruption of the Slain2 gene in mouse cell lines. Each plasmid co-expresses a unique single guide RNA (sgRNA) targeting a distinct site within the Slain2 together with the Streptococcus pyogenes Cas9 nuclease. The plasmids also encode GFP, allowing fluorescent identification and enrichment of successfully transfected cells by fluorescence microscopy or flow cytometry.
The multi-guide design increases the likelihood of generating insertions or deletions (indels) that disrupt the Slain2 open reading frame following Cas9-mediated double-strand break formation. DNA breaks introduced by the CRISPR/Cas9 system are repaired through endogenous non-homologous end joining (NHEJ) pathways, frequently resulting in frameshift mutations that abolish SLAIN2 protein expression.
This CRISPR knockout system enables efficient generation of Slain2-deficient cell models for investigation of SLAIN2 signaling, functional genomics studies, cancer biology research, and evaluation of therapeutic responses in human cell lines.
Key Features
- sgRNAs targeting Slain2 exon(s) critical for SLAIN2 function
- Co-expression of SpCas9 and sgRNA from a single plasmid for simplified delivery
- GFP reporter for identification of transfected cells
- Pool of plasmids targeting multiple Slain2 genomic sites to improve knockout efficiency
- Compatible with delivery by transfection
Design Variants
CRISPRs +/- HDRs
- gRNAs encoded by SLAIN2 CRISPR/Cas9 KO Plasmid (m) and SLAIN2 CRISPR/Cas9 KO Plasmid (m2) target distinct sites within the Slain2 locus. One or both targeting designs may be available. See Related Products for availability.
- HDR donor constructs encoded by SLAIN2 HDR Plasmid (m) and SLAIN2 HDR Plasmid (m2) contain a puromycin resistance cassette and an RFP reporter flanked by Slain2 homology arms to support homology-directed repair at defined Slain2 target sites corresponding to the CRISPR/Cas9 KO designs. HDR donor availability may vary. See Related Products for availability.
For Research Use Only. Not Intended for Diagnostic or Therapeutic Use.