Slac2-a Antibody (C-3): sc-365735
- Slac2-a Antibody (C-3) is a mouse monoclonal IgG1 λ Slac2-a antibody, cited in 2 publications, provided at 200 µg/ml
- raised against amino acids 291-590 mapping at the C-terminus of Slp homologue lacking C2 domains-a of mouse origin
- Slac2-a Antibody (C-3) is recommended for detection of Slac2-a of mouse origin by WB, IP, IF and ELISA
- Anti-Slac2-a Antibody (C-3) is available conjugated to agarose for IP; HRP for WB, IHC(P) and ELISA; and to either phycoerythrin or FITC for IF, IHC(P) and FCM
- also available conjugated to Alexa Fluor® 488, Alexa Fluor® 546, Alexa Fluor® 594 or Alexa Fluor® 647 for WB (RGB), IF, IHC(P) and FCM, and for use with RGB fluorescent imaging systems, such as iBright™ FL1000, FluorChem™, Typhoon, Azure and other comparable systems
- also available conjugated to Alexa Fluor® 680 or Alexa Fluor® 790 for WB (NIR), IF and FCM; for use with Near-Infrared (NIR) detection systems, such as LI-COR®Odyssey®, iBright™ FL1000, FluorChem™, Typhoon, Azure and other comparable systems
- m-IgGλ BP-HRP (mouse IgGλ binding protein-HRP) is the preferred secondary detection reagent for Slac2-a Antibody (C-3) for WB applications. This reagent is now offered in a bundle with Slac2-a Antibody (C-3) (see ordering information below). For additional m-IgGλ BP conjugates see our complete list of Mouse IgG Binding Proteins.
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Slac2-a Antibody (C-3) is a mouse monoclonal IgG1 antibody that detects Slac2-a of mouse origin by western blotting (WB), immunoprecipitation (IP), immunofluorescence (IF), and enzyme-linked immunosorbent assay (ELISA). Slac2-a (C-3) antibody is available in both non-conjugated and various conjugated forms, including agarose, horseradish peroxidase (HRP), phycoerythrin (PE), fluorescein isothiocyanate (FITC), and multiple Alexa Fluor® conjugates. Slac2-a, also known as synaptotagmin-like protein (Slp) homolog lacking C2 domains-a, plays a crucial role in linking Rab27A on melanosomes with Myosin VA in melanocytes, facilitating the transport of pigment and cytotoxic granules. This interaction is vital for proper cellular function, as the stable formation of the Slac2-a, Rab27A, and Myosin VA complex is essential for the movement of these granules within the cell. Disruptions or mutations in the binding domains of these proteins can lead to significant consequences, including albinism and severe immune disorders, highlighting the importance of Slac2-a in maintaining cellular integrity and function.
Alexa Fluor® is a trademark of Molecular Probes Inc., OR., USA
LI-COR® and Odyssey® are registered trademarks of LI-COR Biosciences
Slac2-a Antibody (C-3) References:
- The Slp homology domain of synaptotagmin-like proteins 1-4 and Slac2 functions as a novel Rab27A binding domain. | Kuroda, TS., et al. 2002. J Biol Chem. 277: 9212-8. PMID: 11773082
- Slac2-a/melanophilin, the missing link between Rab27 and myosin Va: implications of a tripartite protein complex for melanosome transport. | Fukuda, M., et al. 2002. J Biol Chem. 277: 12432-6. PMID: 11856727
- Synaptotagmin-like protein (Slp) homology domain 1 of Slac2-a/melanophilin is a critical determinant of GTP-dependent specific binding to Rab27A. | Fukuda, M. 2002. J Biol Chem. 277: 40118-24. PMID: 12189142
- Slac2-c (synaptotagmin-like protein homologue lacking C2 domains-c), a novel linker protein that interacts with Rab27, myosin Va/VIIa, and actin. | Fukuda, M. and Kuroda, TS. 2002. J Biol Chem. 277: 43096-103. PMID: 12221080
- Distinct Rab binding specificity of Rim1, Rim2, rabphilin, and Noc2. Identification of a critical determinant of Rab3A/Rab27A recognition by Rim2. | Fukuda, M. 2003. J Biol Chem. 278: 15373-80. PMID: 12578829
- The actin-binding domain of Slac2-a/melanophilin is required for melanosome distribution in melanocytes. | Kuroda, TS., et al. 2003. Mol Cell Biol. 23: 5245-55. PMID: 12861011
- Rabphilin and Noc2 are recruited to dense-core vesicles through specific interaction with Rab27A in PC12 cells. | Fukuda, M., et al. 2004. J Biol Chem. 279: 13065-75. PMID: 14722103
- Missense mutations in the globular tail of myosin-Va in dilute mice partially impair binding of Slac2-a/melanophilin. | Fukuda, M. and Kuroda, TS. 2004. J Cell Sci. 117: 583-91. PMID: 14730011
- Slac2-a/melanophilin contains multiple PEST-like sequences that are highly sensitive to proteolysis. | Fukuda, M. and Itoh, T. 2004. J Biol Chem. 279: 22314-21. PMID: 15145961
- Rab27A-binding protein Slp2-a is required for peripheral melanosome distribution and elongated cell shape in melanocytes. | Kuroda, TS. and Fukuda, M. 2004. Nat Cell Biol. 6: 1195-203. PMID: 15543135
Ordering Information
| Product Name | Catalog # | UNIT | Price | Qty | FAVORITES | |
Slac2-a Antibody (C-3) | sc-365735 | 200 µg/ml | $316.00 | |||
Slac2-a Antibody (C-3): m-IgGλ BP-HRP Bundle | sc-522346 | 200 µg Ab, 40 µg BP | $354.00 | |||
Slac2-a Antibody (C-3) AC | sc-365735 AC | 500 µg/ml, 25% agarose | $416.00 | |||
Slac2-a Antibody (C-3) HRP | sc-365735 HRP | 200 µg/ml | $316.00 | |||
Slac2-a Antibody (C-3) FITC | sc-365735 FITC | 200 µg/ml | $330.00 | |||
Slac2-a Antibody (C-3) PE | sc-365735 PE | 200 µg/ml | $343.00 | |||
Slac2-a Antibody (C-3) Alexa Fluor® 488 | sc-365735 AF488 | 200 µg/ml | $357.00 | |||
Slac2-a Antibody (C-3) Alexa Fluor® 546 | sc-365735 AF546 | 200 µg/ml | $357.00 | |||
Slac2-a Antibody (C-3) Alexa Fluor® 594 | sc-365735 AF594 | 200 µg/ml | $357.00 | |||
Slac2-a Antibody (C-3) Alexa Fluor® 647 | sc-365735 AF647 | 200 µg/ml | $357.00 | |||
Slac2-a Antibody (C-3) Alexa Fluor® 680 | sc-365735 AF680 | 200 µg/ml | $357.00 | |||
Slac2-a Antibody (C-3) Alexa Fluor® 790 | sc-365735 AF790 | 200 µg/ml | $357.00 |