SKAP Antibody (H-2): sc-398914
- SKAP Antibody (H-2) is a mouse monoclonal IgG1 κ provided at 200 µg/ml
- specific for an epitope mapping between amino acids 82-106 within an internal region of SKAP of mouse origin
- Anti-SKAP Antibody (H-2) is recommended for detection of SKAP of mouse and rat origin by WB, IP, IF and ELISA
- Anti-SKAP Antibody (H-2) is available conjugated to agarose for IP; HRP for WB, IHC(P) and ELISA; and to either phycoerythrin or FITC for IF, IHC(P) and FCM
- also available conjugated to Alexa Fluor® 488, Alexa Fluor® 546, Alexa Fluor® 594 or Alexa Fluor® 647 for WB (RGB), IF, IHC(P) and FCM, and for use with RGB fluorescent imaging systems, such as iBright™ FL1000, FluorChem™, Typhoon, Azure and other comparable systems
- also available conjugated to Alexa Fluor® 680 or Alexa Fluor® 790 for WB (NIR), IF and FCM; for use with Near-Infrared (NIR) detection systems, such as LI-COR®Odyssey®, iBright™ FL1000, FluorChem™, Typhoon, Azure and other comparable systems
- m-IgG Fc BP-HRP, m-IgG1 BP-HRP and m-IgGκ BP-HRP are the preferred secondary detection reagents for SKAP Antibody (H-2) for WB applications. These reagents are now offered in bundles with SKAP Antibody (H-2) (see ordering information below).
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SEE ALSO...
SKAP Antibody (H-2) is a mouse monoclonal IgG1 kappa light chain antibody that detects SKAP in mouse and rat samples through applications such as western blotting (WB), immunoprecipitation (IP), immunofluorescence (IF), and enzyme-linked immunosorbent assay (ELISA). Anti-SKAP antibody (H-2) is available in both non-conjugated and various conjugated forms, including agarose, horseradish peroxidase (HRP), phycoerythrin (PE), fluorescein isothiocyanate (FITC), and multiple Alexa Fluor® conjugates. The small kinetochore associated protein (SKAP), also known as KNSTRN, plays a pivotal role in the mitotic process by ensuring proper chromosome alignment and segregation during cell division. Located at the kinetochore, SKAP is crucial for stabilizing microtubule-kinetochore attachments, which is essential for accurate chromosome segregation. SKAP interacts with SPAG5, forming a complex that localizes to the ends of microtubules, thereby maintaining spindle pole architecture and regulating the metaphase-to-anaphase transition. Disruptions in SKAP function due to mutations in its encoding gene, located on human chromosome 15q15.1, can lead to severe consequences, including the development of cutaneous squamous cell carcinomas. Additionally, SKAP exists in three alternatively spliced isoforms and is down-regulated by nitric oxide, highlighting its complex regulatory mechanisms and importance in cellular processes.
Alexa Fluor® is a trademark of Molecular Probes Inc., OR., USA
LI-COR® and Odyssey® are registered trademarks of LI-COR Biosciences
SKAP Antibody (H-2) References:
- Analysis of differentially expressed genes in nitric oxide-exposed human monocytic cells. | Turpaev, K., et al. 2005. Free Radic Biol Med. 38: 1392-400. PMID: 15855057
- SKAP associates with kinetochores and promotes the metaphase-to-anaphase transition. | Fang, L., et al. 2009. Cell Cycle. 8: 2819-27. PMID: 19667759
- Initial characterization of the human central proteome. | Burkard, TR., et al. 2011. BMC Syst Biol. 5: 17. PMID: 21269460
- The astrin-kinastrin/SKAP complex localizes to microtubule plus ends and facilitates chromosome alignment. | Dunsch, AK., et al. 2011. J Cell Biol. 192: 959-68. PMID: 21402792
- CENP-E kinesin interacts with SKAP protein to orchestrate accurate chromosome segregation in mitosis. | Huang, Y., et al. 2012. J Biol Chem. 287: 1500-9. PMID: 22110139
- Mitotic regulator SKAP forms a link between kinetochore core complex KMN and dynamic spindle microtubules. | Wang, X., et al. 2012. J Biol Chem. 287: 39380-90. PMID: 23035123
- Small kinetochore associated protein (SKAP) promotes UV-induced cell apoptosis through negatively regulating pre-mRNA processing factor 19 (Prp19). | Lu, S., et al. 2014. PLoS One. 9: e92712. PMID: 24718257
- Recurrent point mutations in the kinetochore gene KNSTRN in cutaneous squamous cell carcinoma. | Lee, CS., et al. 2014. Nat Genet. 46: 1060-2. PMID: 25194279
Ordering Information
| Product Name | Catalog # | UNIT | Price | Qty | FAVORITES | |
SKAP Antibody (H-2) | sc-398914 | 200 µg/ml | $316.00 | |||
SKAP Antibody (H-2): m-IgG Fc BP-HRP Bundle | sc-530966 | 200 µg Ab; 10 µg BP | $354.00 | |||
SKAP Antibody (H-2): m-IgGκ BP-HRP Bundle | sc-524533 | 200 µg Ab, 40 µg BP | $354.00 | |||
SKAP Antibody (H-2): m-IgG1 BP-HRP Bundle | sc-544339 | 200 µg Ab; 20 µg BP | $354.00 | |||
SKAP Antibody (H-2) AC | sc-398914 AC | 500 µg/ml, 25% agarose | $416.00 | |||
SKAP Antibody (H-2) HRP | sc-398914 HRP | 200 µg/ml | $316.00 | |||
SKAP Antibody (H-2) FITC | sc-398914 FITC | 200 µg/ml | $330.00 | |||
SKAP Antibody (H-2) PE | sc-398914 PE | 200 µg/ml | $343.00 | |||
SKAP Antibody (H-2) Alexa Fluor® 488 | sc-398914 AF488 | 200 µg/ml | $357.00 | |||
SKAP Antibody (H-2) Alexa Fluor® 546 | sc-398914 AF546 | 200 µg/ml | $357.00 | |||
SKAP Antibody (H-2) Alexa Fluor® 594 | sc-398914 AF594 | 200 µg/ml | $357.00 | |||
SKAP Antibody (H-2) Alexa Fluor® 647 | sc-398914 AF647 | 200 µg/ml | $357.00 | |||
SKAP Antibody (H-2) Alexa Fluor® 680 | sc-398914 AF680 | 200 µg/ml | $357.00 | |||
SKAP Antibody (H-2) Alexa Fluor® 790 | sc-398914 AF790 | 200 µg/ml | $357.00 | |||
SKAP (H-2) Neutralizing Peptide | sc-398914 P | 100 µg/0.5 ml | $68.00 |