Ska3 Antibody (B-1): sc-390326

Ska3 (B-1) is a mouse monoclonal IgG1 kappa light chain antibody that detects Ska3 protein of mouse, rat and human origin by western blotting (WB), immunoprecipitation (IP), immunofluorescence (IF) and enzyme-linked immunosorbent assay (ELISA). Ska3 (B-1) antibody is available as both non-conjugated form, as well as multiple conjugated forms, including agarose, horseradish peroxidase (HRP), phycoerythrin (PE), fluorescein isothiocyanate (FITC) and multiple Alexa Fluor® conjugates. Ska3, also designated C13orf3 or RAMA1, is a 412 amino acid protein that belongs to the RAMA1 family of proteins. A component of the SKA1 complex, Ska3 localizes to the outer kinetochore and spindle microtubules during mitosis. The SKA1 complex is a microtubule-binding subcomplex of the outer kinetochore and is composed of two Ska1-Ska2 heterodimers, each heterodimer interacting with a Ska3 homodimer. Within the complex, which is important for chromosome segregation and facilitates microsphere movement along microtubules, Ska3 acts as a mediator of microtubule-stimulated oligomerization. The gene encoding for Ska3 maps to chromosome 13. Comprising nearly 4% of human DNA, chromosome 13 contains around 114 million base pairs and 400 genes. Key tumor suppressor genes on chromosome 13 include the breast cancer susceptibility gene, BRCA2, and the RB1 (retinoblastoma) gene. RB1 encodes a crucial tumor suppressor protein which, when defective, leads to malignant growth in the retina and has been implicated in a variety of other cancers. The gene SLITRK1, which is associated with Tourette syndrome, is on chromosome 13. As with most chromosomes, polysomy of part or all of chromosome 13 is deleterious to development and decreases the odds of survival. Trisomy 13, also known as Patau syndrome, is quite deadly and the few who survive past one year suffer from permanent neurologic defects, difficulty eating and vulnerability to serious respiratory infections.

Alexa Fluor^® is a trademark of Molecular Probes Inc., OR., USA

LI-COR^® and Odyssey^® are registered trademarks of LI-COR Biosciences

Ska3 Antibody (B-1) References:

1. The DNA sequence and analysis of human chromosome 13.  |  Dunham, A., et al. 2004. Nature. 428: 522-8. PMID: 15057823
2. A new gene for Tourette's syndrome: a window into causal mechanisms?  |  Grados, MA. and Walkup, JT. 2006. Trends Genet. 22: 291-3. PMID: 16678301
3. RB and cell cycle progression.  |  Giacinti, C. and Giordano, A. 2006. Oncogene. 25: 5220-7. PMID: 16936740
4. Examination of the SLITRK1 gene in Caucasian patients with Tourette syndrome.  |  Deng, H., et al. 2006. Acta Neurol Scand. 114: 400-2. PMID: 17083340
5. Non-disjunction of chromosome 13.  |  Bugge, M., et al. 2007. Hum Mol Genet. 16: 2004-10. PMID: 17584770
6. Variable expressivity in Patau syndrome is not all related to trisomy 13 mosaicism.  |  Hsu, HF. and Hou, JW. 2007. Am J Med Genet A. 143A: 1739-48. PMID: 17603803
7. The origin of trisomy 13.  |  Hall, HE., et al. 2007. Am J Med Genet A. 143A: 2242-8. PMID: 17853475
8. Crystal structure of the retinoblastoma protein N domain provides insight into tumor suppression, ligand interaction, and holoprotein architecture.  |  Hassler, M., et al. 2007. Mol Cell. 28: 371-85. PMID: 17996702
9. BRCA2: a universal recombinase regulator.  |  Thorslund, T. and West, SC. 2007. Oncogene. 26: 7720-30. PMID: 18066084

Monoclonal antibodies for mammalian and non-mammalian protein targets represent essentially all targets covered by polyclonal antibodies. Over 8,300 of our antibodies are offered as ImmunoCruz^® conjugates which eliminates the need for a secondary antibody.

Santa Cruz Biotechnology antibodies have over 360,000 research citations. Anti-Ska3 Antibody (B-1) has 4 citations in a variety of scientific publications.

Primary antibodies like Anti-Ska3 Antibody (B-1) for mammalian target proteins are recommended for the detection of a range of mammalian species, primarily of mouse, rat and human species. Many mammalian antibodies are also ideal for veterinary research and reactive with ovine, porcine, caprine, feline, canine, bovine and equine protein targets. Santa Cruz Biotechnology primary monoclonal antibodies are suitable for laboratory research applications and are not intended for diagnostic or therapeutic use.

Ska3 Antibody (B-1) Specifications

Alternate Product Name: Ska3

Product Clone Name: B-1

Classification: Primary Antibody

Clonality: Monoclonal Antibody

Antibody Isotype: IgG₁ kappa light chain

Amino Acids: 154-166

Epitope Target: Ska3

Epitope Species: human

Product Reactivity/Detection: Ska3

Species Reactivity/Detection: mouse, rat, human

Additional Mammalian Reactivity: equine, canine and bovine

Additional Non-Mammalian Reactivity: N/A

Applications: WB, IP, IF, ELISA

Vialing Information: Each vial contains 200 µg IgG₁ kappa light chain in 1.0 ml of PBS with < 0.1% sodium azide and 0.1% gelatin

Research Citations: 4 citations in various scientific publications

Available Conjugates/Forms for Ska3 Antibody (B-1): AC: Agarose, AF488: Alexa Fluor^® 488, AF546: Alexa Fluor^® 546, AF594: Alexa Fluor^® 594, AF647: Alexa Fluor^® 647, AF680: Alexa Fluor^® 680, AF790: Alexa Fluor^® 790, FITC: Fluoroscein, HRP: Horseradish Peroxidase, P: Blocking Peptide, PE: Phycoerythrin