Not CRISPR data: the image illustrates antibody quality for visualizing CRISPR experiment results.
Not CRISPR data: the image illustrates antibody quality for visualizing CRISPR experiment results.
Six2 CRISPR/Cas9 KO Plasmid (m): sc-422957
- Target species: mouse
- 20 µg of transfection-ready, purified plasmid DNA; Suitable for up to 20 transfections
- Six2 CRISPR/Cas9 Knockout (KO) Plasmid (m) is a pool of plasmids, each encoding Cas9 nuclease and a target-specific 20 nt guide RNA (gRNA) designed for maximum knockout efficiency using sequences derived from the GeCKO v2 library
- gRNA sequences direct Cas9 to induce site-specific double-strand breaks (DSBs) in the Six2 genomic locus, resulting in gene knockout through non-homologous end joining (NHEJ)
- The puromycin resistance and RFP genes are flanked by LoxP sites, enabling removal of selection markers via Cre recombinase (Cre Vector: sc-418923) after establishing stable knockout cell lines
- Following transfection, gene knockout efficiency can be assayed by WB, IF or IHC using antibody: Six2 Antibody (H-4): sc-377193
Ordering Information
| Product Name | Catalog # | UNIT | Price | Qty | FAVORITES | |
Six2 CRISPR/Cas9 KO Plasmid (m) | sc-422957 | 20 µg | $397.00 |
Overview
Six2 (SIX homeobox 2) is a homeodomain transcription factor that regulates cell fate decisions during embryonic development, with prominent roles in kidney organogenesis and maintenance of nephron progenitor populations. It functions within transcriptional networks that interface with WNT/β-catenin, FGF, and BMP signaling to balance progenitor self-renewal and differentiation. In mouse models, altered Six2 activity disrupts nephrogenesis and can lead to congenital anomalies of the kidney and urinary tract, making it a key gene for studying developmental pathways. Six2 is also used as a marker and regulator in lineage-tracing and differentiation studies of renal and mesenchymal progenitors.
Six2 CRISPR/Cas9 KO Plasmid (m) is a pool of plasmids designed for targeted disruption of the Six2 gene in mouse cell lines. Each plasmid co-expresses a unique single guide RNA (sgRNA) targeting a distinct site within the Six2 together with the Streptococcus pyogenes Cas9 nuclease. The plasmids also encode GFP, allowing fluorescent identification and enrichment of successfully transfected cells by fluorescence microscopy or flow cytometry.
The multi-guide design increases the likelihood of generating insertions or deletions (indels) that disrupt the Six2 open reading frame following Cas9-mediated double-strand break formation. DNA breaks introduced by the CRISPR/Cas9 system are repaired through endogenous non-homologous end joining (NHEJ) pathways, frequently resulting in frameshift mutations that abolish Six2 protein expression.
This CRISPR knockout system enables efficient generation of Six2-deficient cell models for investigation of Six2 signaling, functional genomics studies, cancer biology research, and evaluation of therapeutic responses in human cell lines.
Key Features
- sgRNAs targeting Six2 exon(s) critical for Six2 function
- Co-expression of SpCas9 and sgRNA from a single plasmid for simplified delivery
- GFP reporter for identification of transfected cells
- Pool of plasmids targeting multiple Six2 genomic sites to improve knockout efficiency
- Compatible with delivery by transfection
Design Variants
CRISPRs +/- HDRs
- gRNAs encoded by Six2 CRISPR/Cas9 KO Plasmid (m) and Six2 CRISPR/Cas9 KO Plasmid (m2) target distinct sites within the Six2 locus. One or both targeting designs may be available. See Related Products for availability.
- HDR donor constructs encoded by Six2 HDR Plasmid (m) and Six2 HDR Plasmid (m2) contain a puromycin resistance cassette and an RFP reporter flanked by Six2 homology arms to support homology-directed repair at defined Six2 target sites corresponding to the CRISPR/Cas9 KO designs. HDR donor availability may vary. See Related Products for availability.
For Research Use Only. Not Intended for Diagnostic or Therapeutic Use.