SIRT2 Double Nickase Plasmid (m): sc-425703-NIC
- Target species: mouse
- 20 µg of transfection-ready, purified plasmid DNA; Suitable for up to 20 transfections
- SIRT2 Double Nickase Plasmid (m) consists of a pair of plasmids each encoding a D10A mutated Cas9 nuclease and a target-specific 20 nt guide RNA (gRNA) designed to knockout gene expression with greater specificity than its CRISPR/Cas9 KO counterpart
- Paired gRNA sequences are offset by approximately 20 bp to allow for specific Cas9-mediated double nicking of the genomic DNA, which mimics a DSB
- One plasmid in the pair contains a puromycin-resistance gene for selection; the other plasmid in the pair contains a GFP marker to visually confirm transfection
- SIRT2 Double Nickase Plasmid (m) and SIRT2 Double Nickase Plasmid (m2) encode distinct paired gRNA designs targeting Sirt2. One or both designs may be available
- Following transfection, gene knockout efficiency can be assayed by WB, IF or IHC using antibody: SIRT2 Antibody (A-5): sc-28298
Ordering Information
| Product Name | Catalog # | UNIT | Price | Qty | FAVORITES | |
SIRT2 Double Nickase Plasmid (m) | sc-425703-NIC | 20 µg | $410.00 |
Mouse Sirt2 encodes the NAD⁺-dependent deacetylase SIRT2, a predominantly cytosolic sirtuin that also shuttles to the nucleus and regulates acetylation of key substrates including α-tubulin and histones. Through control of microtubule dynamics, cell-cycle progression, chromatin organization, and stress-responsive metabolic signaling, SIRT2 influences processes such as mitotic fidelity, inflammatory signaling, and neuronal homeostasis. SIRT2 activity intersects with pathways linked to energy sensing and proteostasis, including crosstalk with FOXO- and NF-κB–associated programs and regulation of protein acetylation states that impact autophagy and oxidative stress responses. Altered SIRT2 expression or function has been studied in the context of neurodegeneration, metabolic dysfunction, and cancer-related phenotypes, making it a useful node for mechanistic studies in mouse models and primary cells.
SIRT2 Double Nickase Plasmid (m) consists of a matched pair of plasmids engineered for high-specificity editing of the Sirt2 locus in mouse cell lines. Each plasmid expresses a Cas9 D10A nickase and a distinct sgRNA targeting opposite DNA strands within Sirt2. When directed to adjacent sites on opposite DNA strands, the two nickases generate offset single-strand nicks that together produce a staggered double-strand break, requiring coordinated on-target activity from both guides. The resulting DNA break is resolved by endogenous cellular repair pathways, most commonly through non-homologous end joining (NHEJ), leading to insertions or deletions that disrupt Sirt2 function. By requiring dual sgRNA engagement at the target locus, the double nicking approach enhances editing specificity and provides a complementary CRISPR strategy for applications where additional control over targeting precision is desired.
To support efficient identification of edited cells, one plasmid encodes GFP for fluorescent visualization of transfected populations, while the companion plasmid carries a puromycin resistance gene for antibiotic selection. Together, these features support efficient enrichment of co-transfected populations and simplify the validation of Sirt2-disrupted clones.
For Research Use Only. Not Intended for Diagnostic or Therapeutic Use.