SIRT1 (h): 293T Lysate: sc-113797

SIRT1 (h): 293T Lysate is derived from the human embryonic kidney 293T cell line, which has been genetically engineered to express human Sirtuin 1 (SIRT1). SIRT1 is a NAD+-dependent deacetylase that plays a critical role in regulating cellular processes such as metabolism, aging, and stress response. The 293T cell line, known for its high transfection efficiency and robust protein expression, is an ideal source for producing SIRT1 lysates. In research, SIRT1 (h): 293T Lysate is extensively used to study the molecular mechanisms by which SIRT1 regulates gene expression and cellular function. SIRT1 deacetylates a variety of substrate proteins, including transcription factors like p53, NF-κB, and FOXO, thereby modulating their activity and stability. Researchers utilize this lysate in biochemical assays such as Western blotting, immunoprecipitation, and deacetylation assays to analyze SIRT1 activity, substrate specificity, and interaction partners. Furthermore, SIRT1 (h): 293T Lysate is employed in chromatin immunoprecipitation (ChIP) assays to investigate the role of SIRT1 in chromatin remodeling and gene silencing. This lysate is also instrumental in functional studies aimed at understanding the crosstalk between SIRT1 and other signaling pathways, including those involved in energy metabolism and oxidative stress. By using SIRT1 (h): 293T Lysate, researchers can gain insights into the diverse biological functions of SIRT1 and its impact on cellular homeostasis and longevity.