SIRP-γ Antibody (OX117): sc-53112

SIRP-γ Antibody (OX117) is a mouse monoclonal IgG1 kappa light chain antibody that detects SIRP-gamma protein of mouse, rat, and human origin by western blotting (WB), immunoprecipitation (IP), and flow cytometry (FCM). SIRP-γ (OX117) antibody is available in both non-conjugated and various conjugated forms, including agarose, horseradish peroxidase (HRP), phycoerythrin (PE), fluorescein isothiocyanate (FITC), and multiple Alexa Fluor® conjugates. The SIRP family, which includes SIRP-γ, plays a crucial role in immune regulation, particularly in T cell signaling. SIRP-γ is predominantly expressed on T cells and a subset of B cells, where SIRP-γ interacts with CD47, a protein that provides a "don′t eat me" signal to macrophages, thereby inhibiting phagocytosis. This interaction is vital for maintaining immune homeostasis and preventing autoimmunity. The unique structure of SIRP-γ, which includes an extracellular domain with immunoglobulin-like motifs, allows SIRP-γ to effectively mediate these interactions, influencing T cell activation and response to external stimuli. Understanding SIRP-γ function and interactions is essential for developing therapeutic strategies targeting immune responses in various diseases, including cancer and autoimmune disorders.

Alexa Fluor^® is a trademark of Molecular Probes Inc., OR., USA

LI-COR^® and Odyssey^® are registered trademarks of LI-COR Biosciences

SIRP-γ Antibody (OX117) References:

1. Inhibition of EGFR-mediated phosphoinositide-3-OH kinase (PI3-K) signaling and glioblastoma phenotype by signal-regulatory proteins (SIRPs).  |  Wu, CJ., et al. 2000. Oncogene. 19: 3999-4010. PMID: 10962556
2. Bidirectional negative regulation of human T and dendritic cells by CD47 and its cognate receptor signal-regulator protein-alpha: down-regulation of IL-12 responsiveness and inhibition of dendritic cell activation.  |  Latour, S., et al. 2001. J Immunol. 167: 2547-54. PMID: 11509594
3. Human lymphocytes interact directly with CD47 through a novel member of the signal regulatory protein (SIRP) family.  |  Brooke, G., et al. 2004. J Immunol. 173: 2562-70. PMID: 15294972
4. Transcriptional regulation of signal regulatory protein alpha1 inhibitory receptors by epidermal growth factor receptor signaling.  |  Kapoor, GS., et al. 2004. Cancer Res. 64: 6444-52. PMID: 15374953
5. SIRPbeta1 is expressed as a disulfide-linked homodimer in leukocytes and positively regulates neutrophil transepithelial migration.  |  Liu, Y., et al. 2005. J Biol Chem. 280: 36132-40. PMID: 16081415
6. Identification of the major SHPTP2-binding protein that is tyrosine-phosphorylated in response to insulin.  |  Yamauchi, K., et al. 1995. J Biol Chem. 270: 17716-22. PMID: 7629070
7. A novel membrane glycoprotein, SHPS-1, that binds the SH2-domain-containing protein tyrosine phosphatase SHP-2 in response to mitogens and cell adhesion.  |  Fujioka, Y., et al. 1996. Mol Cell Biol. 16: 6887-99. PMID: 8943344
8. A family of proteins that inhibit signalling through tyrosine kinase receptors.  |  Kharitonenkov, A., et al. 1997. Nature. 386: 181-6. PMID: 9062191
9. Growth hormone regulation of SIRP and SHP-2 tyrosyl phosphorylation and association.  |  Stofega, MR., et al. 1998. J Biol Chem. 273: 7112-7. PMID: 9507023

The preferred SIRP-γ antibody is SIRP-γ Antibody (OX119): sc-53114.