SIRP-α Double Nickase Plasmid (m): sc-422514-NIC
- Target species: mouse
- 20 µg of transfection-ready, purified plasmid DNA; Suitable for up to 20 transfections
- SIRP-α Double Nickase Plasmid (m) consists of a pair of plasmids each encoding a D10A mutated Cas9 nuclease and a target-specific 20 nt guide RNA (gRNA) designed to knockout gene expression with greater specificity than its CRISPR/Cas9 KO counterpart
- Paired gRNA sequences are offset by approximately 20 bp to allow for specific Cas9-mediated double nicking of the genomic DNA, which mimics a DSB
- One plasmid in the pair contains a puromycin-resistance gene for selection; the other plasmid in the pair contains a GFP marker to visually confirm transfection
- SIRP-α Double Nickase Plasmid (m) and SIRP-α Double Nickase Plasmid (m2) encode distinct paired gRNA designs targeting Sirpa. One or both designs may be available
- Following transfection, gene knockout efficiency can be assayed by WB, IF or IHC using antibody: SIRP-α Antibody (C-7): sc-376884
Ordering Information
| Product Name | Catalog # | UNIT | Price | Qty | FAVORITES | |
SIRP-α Double Nickase Plasmid (m) | sc-422514-NIC | 20 µg | $410.00 | |||
SIRP-α Double Nickase Plasmid (m2) | sc-422514-NIC-2 | 20 µg | $410.00 |
Mouse Sirpa encodes signal regulatory protein alpha (SIRP-α), an immunoglobulin superfamily receptor predominantly expressed on myeloid cells where it functions as an inhibitory checkpoint for phagocytosis. Through engagement with CD47, SIRP-α signals via ITIM motifs to recruit SHP-1/2 phosphatases, dampening cytoskeletal rearrangement, integrin signaling, and innate immune activation pathways that regulate engulfment and inflammatory tone. This axis influences macrophage and dendritic cell homeostasis, antigen handling, and clearance of apoptotic cells, and it is widely studied in contexts such as tumor immune evasion, transplantation biology, and autoimmune or inflammatory phenotypes. Strain- and allele-dependent differences in mouse SIRP-α can also shape xenograft compatibility and innate immune responses, making Sirpa a frequent target in immunology and disease-model development.
SIRP-α Double Nickase Plasmid (m) consists of a matched pair of plasmids engineered for high-specificity editing of the Sirpa locus in mouse cell lines. Each plasmid expresses a Cas9 D10A nickase and a distinct sgRNA targeting opposite DNA strands within Sirpa. When directed to adjacent sites on opposite DNA strands, the two nickases generate offset single-strand nicks that together produce a staggered double-strand break, requiring coordinated on-target activity from both guides. The resulting DNA break is resolved by endogenous cellular repair pathways, most commonly through non-homologous end joining (NHEJ), leading to insertions or deletions that disrupt Sirpa function. By requiring dual sgRNA engagement at the target locus, the double nicking approach enhances editing specificity and provides a complementary CRISPR strategy for applications where additional control over targeting precision is desired.
To support efficient identification of edited cells, one plasmid encodes GFP for fluorescent visualization of transfected populations, while the companion plasmid carries a puromycin resistance gene for antibiotic selection. Together, these features support efficient enrichment of co-transfected populations and simplify the validation of Sirpa-disrupted clones.
For Research Use Only. Not Intended for Diagnostic or Therapeutic Use.