SIRP-α Antibody (C-7): sc-376884
- SIRP-α Antibody (C-7) is a mouse monoclonal IgG2a κ, cited in 5 publications, provided at 200 µg/ml
- specific for an epitope mapping between amino acids 475-503 at the C-terminus of SIRP-α of human origin
- Anti-SIRP-alpha Antibody (C-7) is recommended for detection of SIRP-α of mouse, rat and human origin by WB, IP, IF, IHC(P) and ELISA
- Anti-SIRP-alpha Antibody (C-7) is available conjugated to agarose for IP; HRP for WB, IHC(P) and ELISA; and to either phycoerythrin or FITC for IF, IHC(P) and FCM
- also available conjugated to Alexa Fluor® 488, Alexa Fluor® 546, Alexa Fluor® 594 or Alexa Fluor® 647 for WB (RGB), IF, IHC(P) and FCM, and for use with RGB fluorescent imaging systems, such as iBright™ FL1000, FluorChem™, Typhoon, Azure and other comparable systems
- also available conjugated to Alexa Fluor® 680 or Alexa Fluor® 790 for WB (NIR), IF and FCM; for use with Near-Infrared (NIR) detection systems, such as LI-COR®Odyssey®, iBright™ FL1000, FluorChem™, Typhoon, Azure and other comparable systems
- m-IgGκ BP-HRP is the preferred secondary detection reagent for SIRP-α Antibody (C-7) for WB and IHC(P) applications. This reagent is now offered in a bundle with SIRP-α Antibody (C-7) (see ordering information below). For additional m-IgGκ BP conjugates see our complete list of Mouse IgG Binding Proteins.
SIRP-α Antibody (C-7) is a mouse monoclonal IgG2a kappa light chain antibody that detects SIRP-α in mouse, rat, and human samples through applications such as western blotting (WB), immunoprecipitation (IP), immunofluorescence (IF), immunohistochemistry, and enzyme-linked immunosorbent assay (ELISA). SIRP-α (C-7) antibody is available in both non-conjugated and various conjugated forms, including agarose, horseradish peroxidase (HRP), phycoerythrin (PE), fluorescein isothiocyanate (FITC), and multiple Alexa Fluor® conjugates. SIRP-α, a member of the signal-regulatory protein family, plays a crucial role in modulating immune responses by negatively regulating the PI 3-kinase pathway, which is essential for controlling cell motility and survival in response to growth factors and oncogenic signals. SIRP-α is primarily located in the cell membrane, where its extracellular domain features three immunoglobulin-like structures that facilitate interactions with other proteins, while its cytoplasmic region contains four potential tyrosine phosphorylation sites that are critical for downstream signaling. Notably, SIRP-α interacts with the Src homology 2 domain-containing protein-tyrosine phosphatase SHP-2, and when phosphorylated, serves as a substrate for activated receptor tyrosine kinases, thereby influencing various cellular processes. The intricate balance of SIRP-α′s signaling capabilities underscores its importance in maintaining immune homeostasis and preventing aberrant cell proliferation, making anti-SIRP-α antibody (C-7) an invaluable tool for researchers studying these pathways.
Alexa Fluor® is a trademark of Molecular Probes Inc., OR., USA
LI-COR® and Odyssey® are registered trademarks of LI-COR Biosciences
SIRP-α Antibody (C-7) References:
- Glioblastoma Immunotherapy Targeting the Innate Immune Checkpoint CD47-SIRPα Axis. | Hu, J., et al. 2020. Front Immunol. 11: 593219. PMID: 33329583
- Recent Advances of Tumor Therapy Based on the CD47-SIRPα Axis. | Wang, Y., et al. 2022. Mol Pharm. 19: 1273-1293. PMID: 35436123
- Nanoparticle-Mediated CD47-SIRPα Blockade and Calreticulin Exposure for Improved Cancer Chemo-Immunotherapy. | Luo, JQ., et al. 2023. ACS Nano. 17: 8966-8979. PMID: 37133900
- Targeting CD47-SIRPα axis for Hodgkin and non-Hodgkin lymphoma immunotherapy. | Zhao, P., et al. 2024. Genes Dis. 11: 205-217. PMID: 37588232
- Activation of RIG-I/MDA5 Signaling and Inhibition of CD47-SIRPα Checkpoint with a Dual siRNA-Assembled Nanoadjuvant for Robust Cancer Immunotherapy. | Xu, X., et al. 2024. Angew Chem Int Ed Engl. 63: e202318544. PMID: 38194267
- Surfactant protein D prevents mucin overproduction in airway goblet cells via SIRPα. | Hata, K., et al. 2024. Sci Rep. 14: 1799. PMID: 38245585
- Targeting SLC22A5 fosters mitophagy inhibition-mediated macrophage immunity against septic acute kidney injury upon CD47-SIRPα axis blockade. | Jia, Y., et al. 2024. Heliyon. 10: e26791. PMID: 38586373
- Blockade of SIRPα-CD47 axis by anti-SIRPα antibody enhances anti-tumor activity of DXd antibody-drug conjugates. | Sue, M., et al. 2024. PLoS One. 19: e0304985. PMID: 38843278
- Blockade of the CD47/SIRPα checkpoint axis potentiates the macrophage-mediated antitumor efficacy of tafasitamab. | Biedermann, A., et al. 2024. Haematologica. 109: 3928-3940. PMID: 38934068
- Podocyte SIRPα reduction in diabetic nephropathy aggravates podocyte injury by promoting pyruvate kinase M2 nuclear translocation. | Chen, Y., et al. 2024. Redox Biol. 78: 103439. PMID: 39586122
Ordering Information
| Product Name | Catalog # | UNIT | Price | Qty | FAVORITES | |
SIRP-α Antibody (C-7) | sc-376884 | 200 µg/ml | $322.00 | |||
SIRP-α Antibody (C-7): m-IgGκ BP-HRP Bundle | sc-523127 | 200 µg Ab, 40 µg BP | $361.00 | |||
SIRP-α Antibody (C-7) AC | sc-376884 AC | 500 µg/ml, 25% agarose | $424.00 | |||
SIRP-α Antibody (C-7) HRP | sc-376884 HRP | 200 µg/ml | $322.00 | |||
SIRP-α Antibody (C-7) FITC | sc-376884 FITC | 200 µg/ml | $336.00 | |||
SIRP-α Antibody (C-7) PE | sc-376884 PE | 200 µg/ml | $349.00 | |||
SIRP-α Antibody (C-7) Alexa Fluor® 488 | sc-376884 AF488 | 200 µg/ml | $364.00 | |||
SIRP-α Antibody (C-7) Alexa Fluor® 546 | sc-376884 AF546 | 200 µg/ml | $364.00 | |||
SIRP-α Antibody (C-7) Alexa Fluor® 594 | sc-376884 AF594 | 200 µg/ml | $364.00 | |||
SIRP-α Antibody (C-7) Alexa Fluor® 647 | sc-376884 AF647 | 200 µg/ml | $364.00 | |||
SIRP-α Antibody (C-7) Alexa Fluor® 680 | sc-376884 AF680 | 200 µg/ml | $364.00 | |||
SIRP-α Antibody (C-7) Alexa Fluor® 790 | sc-376884 AF790 | 200 µg/ml | $364.00 | |||
SIRP-α (C-7) Neutralizing Peptide | sc-376884 P | 100 µg/0.5 ml | $69.00 |