SIRP-α/β1 Antibody (E-7): sc-55491

SIRP-α/β1 Antibody (E-7) is a mouse monoclonal IgG1 kappa light chain antibody that detects SIRP-α/β1 protein of human origin by western blotting (WB), immunoprecipitation (IP), immunofluorescence (IF), immunohistochemistry with paraffin-embedded sections (IHCP), and enzyme-linked immunosorbent assay (ELISA). SIRP-α/β1 (E-7) antibody is available as a non-conjugated anti-SIRP-α/β1 monoclonal isotype antibody. The SIRP family, which includes SIRP-α and SIRP-β1, plays a crucial role in regulating immune responses and cellular signaling. SIRP-α is primarily located on the cell surface and mediates interactions with CD47, a "don′t eat me" signal that inhibits phagocytosis by macrophages. This interaction maintains immune tolerance and prevents premature clearance of healthy cells. SIRP-α expression on immune cells, particularly in tumor microenvironments, can significantly influence immunotherapy efficacy by shielding cancer cells from immune detection. SIRP-β1, while sharing structural similarities with SIRP-α, lacks the cytoplasmic signaling domain, suggesting distinct roles in immune regulation. Anti-SIRP-α/β1 antibody (E-7) effectively detects these proteins, making this reagent invaluable for researchers studying immune modulation and potential therapeutic targets in cancer and other diseases.

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SIRP-α/β1 Antibody (E-7) References:

1. Inhibition of EGFR-mediated phosphoinositide-3-OH kinase (PI3-K) signaling and glioblastoma phenotype by signal-regulatory proteins (SIRPs).  |  Wu, CJ., et al. 2000. Oncogene. 19: 3999-4010. PMID: 10962556
2. Bidirectional negative regulation of human T and dendritic cells by CD47 and its cognate receptor signal-regulator protein-alpha: down-regulation of IL-12 responsiveness and inhibition of dendritic cell activation.  |  Latour, S., et al. 2001. J Immunol. 167: 2547-54. PMID: 11509594
3. Human lymphocytes interact directly with CD47 through a novel member of the signal regulatory protein (SIRP) family.  |  Brooke, G., et al. 2004. J Immunol. 173: 2562-70. PMID: 15294972
4. Transcriptional regulation of signal regulatory protein alpha1 inhibitory receptors by epidermal growth factor receptor signaling.  |  Kapoor, GS., et al. 2004. Cancer Res. 64: 6444-52. PMID: 15374953
5. SIRPbeta1 is expressed as a disulfide-linked homodimer in leukocytes and positively regulates neutrophil transepithelial migration.  |  Liu, Y., et al. 2005. J Biol Chem. 280: 36132-40. PMID: 16081415
6. OX130 Monoclonal Antibody Recognizes Human SIRPβ1 but Cross-Reacts on SIRPα from One Allele.  |  Hatherley, D., et al. 2016. Monoclon Antib Immunodiagn Immunother. 35: 57-9. PMID: 26871367
7. Anticancer efficacy of monotherapy with antibodies to SIRPα/SIRPβ1 mediated by induction of antitumorigenic macrophages.  |  Sakamoto, M., et al. 2022. Proc Natl Acad Sci U S A. 119: PMID: 34949714
8. An Insertion Within SIRPβ1 Shows a Dual Effect Over Alzheimer's Disease Cognitive Decline Altering the Microglial Response.  |  García-Alberca, JM., et al. 2024. J Alzheimers Dis. 98: 601-618. PMID: 38427484
9. Identification of the major SHPTP2-binding protein that is tyrosine-phosphorylated in response to insulin.  |  Yamauchi, K., et al. 1995. J Biol Chem. 270: 17716-22. PMID: 7629070
10. A novel membrane glycoprotein, SHPS-1, that binds the SH2-domain-containing protein tyrosine phosphatase SHP-2 in response to mitogens and cell adhesion.  |  Fujioka, Y., et al. 1996. Mol Cell Biol. 16: 6887-99. PMID: 8943344
11. A family of proteins that inhibit signalling through tyrosine kinase receptors.  |  Kharitonenkov, A., et al. 1997. Nature. 386: 181-6. PMID: 9062191
12. Growth hormone regulation of SIRP and SHP-2 tyrosyl phosphorylation and association.  |  Stofega, MR., et al. 1998. J Biol Chem. 273: 7112-7. PMID: 9507023

Monoclonal antibodies for mammalian and non-mammalian protein targets represent essentially all targets covered by polyclonal antibodies. Over 8,300 of our antibodies are offered as ImmunoCruz^® conjugates which eliminates the need for a secondary antibody.

Santa Cruz Biotechnology antibodies have over 360,000 research citations. Anti-SIRP-α/β1 Antibody (E-7) has 0 citations in a variety of scientific publications.

Primary antibodies like Anti-SIRP-α/β1 Antibody (E-7) for mammalian target proteins are recommended for the detection of a range of mammalian species, primarily of mouse, rat and human species. Many mammalian antibodies are also ideal for veterinary research and reactive with ovine, porcine, caprine, feline, canine, bovine and equine protein targets. Santa Cruz Biotechnology primary monoclonal antibodies are suitable for laboratory research applications and are not intended for diagnostic or therapeutic use.

SIRP-α/β1 Antibody (E-7) Specifications

Alternate Product Name: SIRP-α/β1

Product Clone Name: E-7

Classification: Primary Antibody

Clonality: Monoclonal Antibody

Antibody Isotype: IgG₁ kappa light chain

Amino Acids: 1-300

Epitope Target: SIRP-α

Epitope Species: human

Product Reactivity/Detection: SIRP-α and β1

Species Reactivity/Detection: human

Additional Mammalian Reactivity: N/A

Additional Non-Mammalian Reactivity: N/A

Applications: WB, IP, IF, IHC(P), ELISA

Vialing Information: Each vial contains 200 µg IgG₁ kappa light chain in 1.0 ml of PBS with < 0.1% sodium azide and 0.1% gelatin

Research Citations: 0 citations in various scientific publications

Available Conjugates/Forms for SIRP-α/β1 Antibody (E-7): N/A