SIRP-α Antibody (SE7C2): sc-23863

SIRP-α Antibody (SE7C2) is a mouse monoclonal IgG1 kappa light chain antibody that detects the SIRP-alpha protein of human origin by western blotting (WB), immunoprecipitation (IP), and flow cytometry (FCM). SIRP-α (SE7C2) antibody is available in both non-conjugated and various conjugated forms, including agarose, horseradish peroxidase (HRP), phycoerythrin (PE), fluorescein isothiocyanate (FITC), and multiple Alexa Fluor® conjugates. SIRP-α, also known as SIRP-α1, SIRP-α2, or SIRP-α3, is a transmembrane glycoprotein that plays a crucial role in regulating immune responses. SIRP-α contains an extracellular domain with three immunoglobulin-like structures and a cytoplasmic region featuring four potential tyrosine phosphorylation sites. SIRP-α is primarily located on the surface of immune cells, where SIRP-α interacts with CD47, a "don′t eat me" signal that inhibits phagocytosis by macrophages. This interaction is vital for maintaining immune homeostasis and preventing autoimmunity, as SIRP-α helps to regulate the balance between immune activation and inhibition. Additionally, SIRP-α has been shown to negatively regulate cellular responses to growth factors and oncogenes, thereby influencing cell survival and motility. SIRP-α′s ability to modulate these pathways underscores its importance in both normal immune function and the pathogenesis of various diseases, including cancer.

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SIRP-α Antibody (SE7C2) References:

1. Inhibition of EGFR-mediated phosphoinositide-3-OH kinase (PI3-K) signaling and glioblastoma phenotype by signal-regulatory proteins (SIRPs).  |  Wu, CJ., et al. 2000. Oncogene. 19: 3999-4010. PMID: 10962556
2. Bidirectional negative regulation of human T and dendritic cells by CD47 and its cognate receptor signal-regulator protein-alpha: down-regulation of IL-12 responsiveness and inhibition of dendritic cell activation.  |  Latour, S., et al. 2001. J Immunol. 167: 2547-54. PMID: 11509594
3. Human lymphocytes interact directly with CD47 through a novel member of the signal regulatory protein (SIRP) family.  |  Brooke, G., et al. 2004. J Immunol. 173: 2562-70. PMID: 15294972
4. Transcriptional regulation of signal regulatory protein alpha1 inhibitory receptors by epidermal growth factor receptor signaling.  |  Kapoor, GS., et al. 2004. Cancer Res. 64: 6444-52. PMID: 15374953
5. SIRPbeta1 is expressed as a disulfide-linked homodimer in leukocytes and positively regulates neutrophil transepithelial migration.  |  Liu, Y., et al. 2005. J Biol Chem. 280: 36132-40. PMID: 16081415
6. Identification of the major SHPTP2-binding protein that is tyrosine-phosphorylated in response to insulin.  |  Yamauchi, K., et al. 1995. J Biol Chem. 270: 17716-22. PMID: 7629070
7. A novel membrane glycoprotein, SHPS-1, that binds the SH2-domain-containing protein tyrosine phosphatase SHP-2 in response to mitogens and cell adhesion.  |  Fujioka, Y., et al. 1996. Mol Cell Biol. 16: 6887-99. PMID: 8943344
8. A family of proteins that inhibit signalling through tyrosine kinase receptors.  |  Kharitonenkov, A., et al. 1997. Nature. 386: 181-6. PMID: 9062191
9. Growth hormone regulation of SIRP and SHP-2 tyrosyl phosphorylation and association.  |  Stofega, MR., et al. 1998. J Biol Chem. 273: 7112-7. PMID: 9507023

Monoclonal antibodies for mammalian and non-mammalian protein targets represent essentially all targets covered by polyclonal antibodies. Over 8,300 of our antibodies are offered as ImmunoCruz^® conjugates which eliminates the need for a secondary antibody.

Santa Cruz Biotechnology antibodies have over 360,000 research citations. Anti-SIRP-α Antibody (SE7C2) has 13 citations in a variety of scientific publications.

Primary antibodies like Anti-SIRP-α Antibody (SE7C2) for mammalian target proteins are recommended for the detection of a range of mammalian species, primarily of mouse, rat and human species. Many mammalian antibodies are also ideal for veterinary research and reactive with ovine, porcine, caprine, feline, canine, bovine and equine protein targets. Santa Cruz Biotechnology primary monoclonal antibodies are suitable for laboratory research applications and are not intended for diagnostic or therapeutic use.

SIRP-α Antibody (SE7C2) Specifications

Alternate Product Name: SIRP-α

Product Clone Name: SE7C2

Classification: Primary Antibody

Clonality: Monoclonal Antibody

Antibody Isotype: IgG₁ kappa light chain

Amino Acids:

Epitope Target: SIRP-α

Epitope Species: human

Product Reactivity/Detection: SIRP-α

Species Reactivity/Detection: human

Additional Mammalian Reactivity: N/A

Additional Non-Mammalian Reactivity: N/A

Applications: WB, IP, FCM

Vialing Information: Each vial contains 200 µg IgG₁ kappa light chain in 1.0 ml of PBS with < 0.1% sodium azide and 0.1% gelatin

Research Citations: 13 citations in various scientific publications

Available Conjugates/Forms for SIRP-α Antibody (SE7C2): AC: Agarose, AF488: Alexa Fluor^® 488, AF546: Alexa Fluor^® 546, AF594: Alexa Fluor^® 594, AF647: Alexa Fluor^® 647, AF680: Alexa Fluor^® 680, AF790: Alexa Fluor^® 790, FITC: Fluoroscein, HRP: Horseradish Peroxidase, PE: Phycoerythrin