SIMP Double Nickase Plasmid (h): sc-404481-NIC
- Target species: human
- 20 µg of transfection-ready, purified plasmid DNA; Suitable for up to 20 transfections
- SIMP Double Nickase Plasmid (h) consists of a pair of plasmids each encoding a D10A mutated Cas9 nuclease and a target-specific 20 nt guide RNA (gRNA) designed to knockout gene expression with greater specificity than its CRISPR/Cas9 KO counterpart
- Paired gRNA sequences are offset by approximately 20 bp to allow for specific Cas9-mediated double nicking of the genomic DNA, which mimics a DSB
- One plasmid in the pair contains a puromycin-resistance gene for selection; the other plasmid in the pair contains a GFP marker to visually confirm transfection
- SIMP Double Nickase Plasmid (h) and SIMP Double Nickase Plasmid (h2) encode distinct paired gRNA designs targeting STT3B. One or both designs may be available
Ordering Information
| Product Name | Catalog # | UNIT | Price | Qty | FAVORITES | |
SIMP Double Nickase Plasmid (h) | sc-404481-NIC | 20 µg | $410.00 | |||
SIMP Double Nickase Plasmid (h2) | sc-404481-NIC-2 | 20 µg | $410.00 |
STT3B encodes a catalytic subunit of the oligosaccharyltransferase complex responsible for N-linked glycosylation in the endoplasmic reticulum, with a prominent role in post-translational modification of glycoproteins that escape co-translational glycan transfer. Through its contribution to protein folding, quality control, and ER-associated degradation, STT3B influences secretory pathway proteostasis and the maturation of membrane and secreted receptors. Perturbation of STT3B-dependent glycosylation can alter receptor trafficking and signaling outputs, linking this pathway to cellular stress responses and phenotypes relevant to glycoprotein homeostasis disorders and tumor biology. As a hub for ER glycoprotein processing, STT3B is frequently investigated in studies of secretory pathway regulation, immune receptor biogenesis, and proteostasis network remodeling.
SIMP Double Nickase Plasmid (h) consists of a matched pair of plasmids engineered for high-specificity editing of the STT3B locus in human cell lines. Each plasmid expresses a Cas9 D10A nickase and a distinct sgRNA targeting opposite DNA strands within STT3B. When directed to adjacent sites on opposite DNA strands, the two nickases generate offset single-strand nicks that together produce a staggered double-strand break, requiring coordinated on-target activity from both guides. The resulting DNA break is resolved by endogenous cellular repair pathways, most commonly through non-homologous end joining (NHEJ), leading to insertions or deletions that disrupt STT3B function. By requiring dual sgRNA engagement at the target locus, the double nicking approach enhances editing specificity and provides a complementary CRISPR strategy for applications where additional control over targeting precision is desired.
To support efficient identification of edited cells, one plasmid encodes GFP for fluorescent visualization of transfected populations, while the companion plasmid carries a puromycin resistance gene for antibiotic selection. Together, these features support efficient enrichment of co-transfected populations and simplify the validation of STT3B-disrupted clones.
For Research Use Only. Not Intended for Diagnostic or Therapeutic Use.