SIM2s Double Nickase Plasmid (h): sc-405609-NIC
- Target species: human
- 20 µg of transfection-ready, purified plasmid DNA; Suitable for up to 20 transfections
- SIM2s Double Nickase Plasmid (h) consists of a pair of plasmids each encoding a D10A mutated Cas9 nuclease and a target-specific 20 nt guide RNA (gRNA) designed to knockout gene expression with greater specificity than its CRISPR/Cas9 KO counterpart
- Paired gRNA sequences are offset by approximately 20 bp to allow for specific Cas9-mediated double nicking of the genomic DNA, which mimics a DSB
- One plasmid in the pair contains a puromycin-resistance gene for selection; the other plasmid in the pair contains a GFP marker to visually confirm transfection
- SIM2s Double Nickase Plasmid (h) and SIM2s Double Nickase Plasmid (h2) encode distinct paired gRNA designs targeting SIM2. One or both designs may be available
- Following transfection, gene knockout efficiency can be assayed by WB, IF or IHC using antibody: SIM2s Antibody (3E6): sc-517035
Ordering Information
| Product Name | Catalog # | UNIT | Price | Qty | FAVORITES | |
SIM2s Double Nickase Plasmid (h) | sc-405609-NIC | 20 µg | $410.00 | |||
SIM2s Double Nickase Plasmid (h2) | sc-405609-NIC-2 | 20 µg | $410.00 |
SIM2 (single-minded family bHLH transcription factor 2) encodes the human SIM2s isoform, a basic helix–loop–helix PAS-domain transcription factor that regulates context-dependent gene expression programs involved in cellular differentiation and developmental patterning. SIM2s functions through PAS-mediated dimerization with partner factors and binding to E-box–like regulatory elements, linking it to transcriptional networks that shape lineage specification, epithelial state, and stress-responsive signaling. Altered SIM2/SIM2s expression has been associated with dysregulated transcriptional control in cancer biology and developmental disorders, supporting its use as a mechanistic node in studies of gene regulation and cell fate. In biomedical research, SIM2s is commonly investigated for its influence on pathway-level outputs such as differentiation-associated transcriptional signatures, epithelial–mesenchymal balance, and proliferation-linked regulatory circuits.
SIM2s Double Nickase Plasmid (h) consists of a matched pair of plasmids engineered for high-specificity editing of the SIM2 locus in human cell lines. Each plasmid expresses a Cas9 D10A nickase and a distinct sgRNA targeting opposite DNA strands within SIM2. When directed to adjacent sites on opposite DNA strands, the two nickases generate offset single-strand nicks that together produce a staggered double-strand break, requiring coordinated on-target activity from both guides. The resulting DNA break is resolved by endogenous cellular repair pathways, most commonly through non-homologous end joining (NHEJ), leading to insertions or deletions that disrupt SIM2 function. By requiring dual sgRNA engagement at the target locus, the double nicking approach enhances editing specificity and provides a complementary CRISPR strategy for applications where additional control over targeting precision is desired.
To support efficient identification of edited cells, one plasmid encodes GFP for fluorescent visualization of transfected populations, while the companion plasmid carries a puromycin resistance gene for antibiotic selection. Together, these features support efficient enrichment of co-transfected populations and simplify the validation of SIM2-disrupted clones.
For Research Use Only. Not Intended for Diagnostic or Therapeutic Use.