Siglec-1 Antibody (IBL-13): sc-65375

Siglec-1 Antibody (IBL-13) is a mouse monoclonal IgG antibody that detects Siglec-1 in mouse samples through immunofluorescence (IF) applications. Siglec-1, also known as CD169, is a crucial sialic acid-binding receptor predominantly expressed in hemopoietic cells, where Siglec-1 plays a significant role in mediating local cell-cell interactions within lymphoid tissues. CD169 is essential for the immune response, facilitating communication between macrophages and other immune cells, thereby influencing the activation and regulation of immune responses. Notably, Siglec-1 exhibits a higher affinity for N-acetylneuraminic acid (Neu5Ac) compared to N-glycolylneuraminic acid (Neu5Gc), which underscores CD169′s specificity in recognizing sialylated ligands. The human Siglec-1 gene, located on chromosome 20p13, encodes a 1,709 amino acid protein, and alternative splicing can produce a soluble isoform that is not membrane-bound, further diversifying CD169′s functional roles. The ability of Siglec-1 to interact with various ligands at contact points between macrophages and other cells highlights CD169′s importance in maintaining immune homeostasis and facilitating effective immune responses. anti-Siglec-1 antibody (IBL-13) is an invaluable tool for researchers studying immune interactions and sialic acid biology in mouse models.

Alexa Fluor^® is a trademark of Molecular Probes Inc., OR., USA

LI-COR^® and Odyssey^® are registered trademarks of LI-COR Biosciences

Siglec-1 Antibody (IBL-13) References:

1. The role of Siglec-1 and SR-BI interaction in the phagocytosis of oxidized low density lipoprotein by macrophages.  |  Xiong, YS., et al. 2013. PLoS One. 8: e58831. PMID: 23520536
2. HIV-1 capture and transmission by dendritic cells: the role of viral glycolipids and the cellular receptor Siglec-1.  |  Izquierdo-Useros, N., et al. 2014. PLoS Pathog. 10: e1004146. PMID: 25033082
3. Siglec-1 and -2 as potential biomarkers in autoimmune disease.  |  Eakin, AJ., et al. 2016. Proteomics Clin Appl. 10: 635-44. PMID: 26752092
4. Inhibition of siglec-1 by lentivirus mediated small interfering RNA attenuates atherogenesis in apoE-deficient mice.  |  Xiong, YS., et al. 2017. Clin Immunol. 174: 32-40. PMID: 27871915
5. Preferential use of Siglec-1 or Siglec-10 by type 1 and type 2 PRRSV strains to infect PK15S1-CD163 and PK15S10-CD163 cells.  |  Xie, J., et al. 2018. Vet Res. 49: 67. PMID: 30021620
6. Tuberculosis-associated IFN-I induces Siglec-1 on tunneling nanotubes and favors HIV-1 spread in macrophages.  |  Dupont, M., et al. 2020. Elife. 9: PMID: 32223897
7. Installation of high-affinity Siglec-1 ligand on tumor surface for macrophage-engaged tumor suppression.  |  Zhang, E., et al. 2021. Bioorg Med Chem Lett. 50: 128328. PMID: 34425200
8. The role of sialic acid-binding immunoglobulin-like-lectin-1 (siglec-1) in immunology and infectious disease.  |  Prenzler, S., et al. 2023. Int Rev Immunol. 42: 113-138. PMID: 34494938
9. CD169 (Siglec-1) as a Robust Human Cell Biomarker of Toll-Like Receptor 9 Agonist Immunotherapy.  |  Lende, SSF., et al. 2022. Front Cell Infect Microbiol. 12: 919097. PMID: 35865810
10. A novel subpopulation of monocytes with a strong interferon signature indicated by SIGLEC-1 is present in patients with in recent-onset type 1 diabetes.  |  Guo, M., et al. 2024. Diabetologia. 67: 623-640. PMID: 38349399

The preferred Siglec-1 antibody is Siglec-1 Antibody (HSn 7D2): sc-53442.