SID-1 Antibody (F-12): sc-390015

SID-1 Antibody (F-12) is a mouse monoclonal IgG1 kappa light chain antibody that detects SID-1 protein of mouse, rat, and human origin by western blotting (WB), immunoprecipitation (IP), immunofluorescence (IF), and enzyme-linked immunosorbent assay (ELISA). SID-1 Antibody (F-12) is available in both non-conjugated and various conjugated forms, including agarose, horseradish peroxidase (HRP), phycoerythrin (PE), fluorescein isothiocyanate (FITC), and multiple Alexa Fluor® conjugates. SID-1 protein, which is 827 amino acids long, is a member of the systemic RNA interference defective-1 (SID1) family of transmembrane proteins, originally identified in Caenorhabditis elegans. SID-1 protein is localized to the cell membrane and features eleven transmembrane domains, indicating a potential role as a channel protein. This structural characteristic suggests that SID-1 may facilitate the uptake of double-stranded RNA (dsRNA), a process particularly important in RNA interference and gene regulation. Notably, SID-1 overexpression has been shown to enhance dsRNA uptake in pancreatic ductal adenocarcinoma cells, highlighting significance in cancer biology and therapeutic applications. Additionally, SID-2, another member of the SID family, is an 832 amino acid protein that also contains multiple transmembrane domains and exists in at least two isoforms due to alternative splicing, further emphasizing the complexity and functional diversity of this protein family.

Alexa Fluor^® is a trademark of Molecular Probes Inc., OR., USA

LI-COR^® and Odyssey^® are registered trademarks of LI-COR Biosciences

SID-1 Antibody (F-12) References:

1. Systemic RNAi in C. elegans requires the putative transmembrane protein SID-1.  |  Winston, WM., et al. 2002. Science. 295: 2456-9. PMID: 11834782
2. Transport of dsRNA into cells by the transmembrane protein SID-1.  |  Feinberg, EH. and Hunter, CP. 2003. Science. 301: 1545-7. PMID: 12970568
3. Functional genomic analysis of RNA interference in C. elegans.  |  Kim, JK., et al. 2005. Science. 308: 1164-7. PMID: 15790806
4. Vibrational energies for the X1A1, A1B1, and B1A1 states of SiH2/SiD2 and related transition probabilities based on global potential energy surfaces.  |  Tokue, I., et al. 2005. J Chem Phys. 122: 144307. PMID: 15847522
5. RNA interference: a mammalian SID-1 homologue enhances siRNA uptake and gene silencing efficacy in human cells.  |  Duxbury, MS., et al. 2005. Biochem Biophys Res Commun. 331: 459-63. PMID: 15850781
6. Systemic RNAi in Caenorhabditis elegans.  |  Hunter, CP., et al. 2006. Cold Spring Harb Symp Quant Biol. 71: 95-100. PMID: 17381285
7. Ectopic expression of systemic RNA interference defective protein in embryonic stem cells.  |  Tsang, SY., et al. 2007. Biochem Biophys Res Commun. 357: 480-6. PMID: 17434453
8. Caenorhabditis elegans SID-2 is required for environmental RNA interference.  |  Winston, WM., et al. 2007. Proc Natl Acad Sci U S A. 104: 10565-70. PMID: 17563372

Monoclonal antibodies for mammalian and non-mammalian protein targets represent essentially all targets covered by polyclonal antibodies. Over 8,300 of our antibodies are offered as ImmunoCruz^® conjugates which eliminates the need for a secondary antibody.

Santa Cruz Biotechnology antibodies have over 360,000 research citations. Anti-SID-1 Antibody (F-12) has 1 citations in a variety of scientific publications.

Primary antibodies like Anti-SID-1 Antibody (F-12) for mammalian target proteins are recommended for the detection of a range of mammalian species, primarily of mouse, rat and human species. Many mammalian antibodies are also ideal for veterinary research and reactive with ovine, porcine, caprine, feline, canine, bovine and equine protein targets. Santa Cruz Biotechnology primary monoclonal antibodies are suitable for laboratory research applications and are not intended for diagnostic or therapeutic use.

SID-1 Antibody (F-12) Specifications

Alternate Product Name: SID-1

Product Clone Name: F-12

Classification: Primary Antibody

Clonality: Monoclonal Antibody

Antibody Isotype: IgG₁ kappa light chain

Amino Acids: 1-95

Epitope Target: SID-1

Epitope Species: mouse

Product Reactivity/Detection: SID-1

Species Reactivity/Detection: mouse, rat, human

Additional Mammalian Reactivity: N/A

Additional Non-Mammalian Reactivity: N/A

Applications: WB, IP, IF, ELISA

Vialing Information: Each vial contains 200 µg IgG₁ kappa light chain in 1.0 ml of PBS with < 0.1% sodium azide and 0.1% gelatin

Research Citations: 1 citations in various scientific publications

Available Conjugates/Forms for SID-1 Antibody (F-12): AC: Agarose, AF488: Alexa Fluor^® 488, AF546: Alexa Fluor^® 546, AF594: Alexa Fluor^® 594, AF647: Alexa Fluor^® 647, AF680: Alexa Fluor^® 680, AF790: Alexa Fluor^® 790, FITC: Fluoroscein, HRP: Horseradish Peroxidase, PE: Phycoerythrin