SIAH-2 Antibody (24E6H3): sc-81787
- SIAH-2 Antibody (24E6H3) is a mouse monoclonal IgG2a κ, cited in 3 publications, provided at 200 µg/ml
- raised against a synthetic peptide corresponding to a region near the N-terminus of SIAH of Drosophila melanogaster origin
- SIAH-2 Antibody (24E6H3) is recommended for detection of SIAH-2 of mouse, rat, human and Drosophila origin by WB, IF and IHC(P); also reactive with additional species, including porcine; non cross-reactive with SIAH-1 of human origin
- Anti-SIAH-2 Antibody (24E6H3) is available conjugated to agarose for IP; HRP for WB, IHC(P) and ELISA; and to either phycoerythrin or FITC for IF, IHC(P) and FCM
- also available conjugated to Alexa Fluor® 488, Alexa Fluor® 546, Alexa Fluor® 594 or Alexa Fluor® 647 for WB (RGB), IF, IHC(P) and FCM, and for use with RGB fluorescent imaging systems, such as iBright™ FL1000, FluorChem™, Typhoon, Azure and other comparable systems
- also available conjugated to Alexa Fluor® 680 or Alexa Fluor® 790 for WB (NIR), IF and FCM; for use with Near-Infrared (NIR) detection systems, such as LI-COR®Odyssey®, iBright™ FL1000, FluorChem™, Typhoon, Azure and other comparable systems
- m-IgG Fc BP-HRP and m-IgG2a BP-HRP are the preferred secondary detection reagents for SIAH-2 Antibody (24E6H3) for WB and IHC(P) applications. These reagents are now offered in bundles with SIAH-2 Antibody (24E6H3) (see ordering information below).
SIAH-2 Antibody (24E6H3) is a mouse monoclonal IgG2a kappa light chain antibody that detects SIAH-2 in mouse, rat, human, and Drosophila samples through applications such as western blotting (WB), immunofluorescence (IF), and immunohistochemistry. Anti-SIAH-2 antibody (24E6H3) is available in both non-conjugated and various conjugated forms, including agarose, horseradish peroxidase (HRP), phycoerythrin (PE), fluorescein isothiocyanate (FITC), and multiple Alexa Fluor® conjugates. SIAH-2, or seven in absentia homolog 2, is an E3 ubiquitin ligase that plays a crucial role in regulating protein degradation via the ubiquitin-proteasome pathway. SIAH-2 is essential for maintaining cellular homeostasis, particularly under stress conditions, as SIAH-2 targets specific substrates for degradation. Notably, SIAH-2 interacts with TRAF2, a key regulator of stress responses and cytokine signaling, facilitating TRAF2 degradation during hypoxic conditions. Additionally, SIAH-2 targets prolyl hydroxylase 3 (PHD3) for degradation in response to low oxygen levels, which is critical for the regulation of hypoxia-inducible factor 1-alpha (HIF-1α) and subsequent cellular adaptations to hypoxia. SIAH-2′s ability to modulate these pathways underscores SIAH-2′s importance in cellular responses to environmental stressors, and SIAH-2 null mice exhibit impaired respiratory responses and reduced hemoglobin levels when subjected to hypoxia, highlighting SIAH-2′s vital role in oxygen homeostasis.
Alexa Fluor® is a trademark of Molecular Probes Inc., OR., USA
LI-COR® and Odyssey® are registered trademarks of LI-COR Biosciences
SIAH-2 Antibody (24E6H3) References:
- Stress-induced decrease in TRAF2 stability is mediated by Siah2. | Habelhah, H., et al. 2002. EMBO J. 21: 5756-65. PMID: 12411493
- Normal p53 function in primary cells deficient for Siah genes. | Frew, IJ., et al. 2002. Mol Cell Biol. 22: 8155-64. PMID: 12417719
- Generation and analysis of Siah2 mutant mice. | Frew, IJ., et al. 2003. Mol Cell Biol. 23: 9150-61. PMID: 14645526
- Siah proteins, HIF prolyl hydroxylases, and the physiological response to hypoxia. | Simon, MC. 2004. Cell. 117: 851-3. PMID: 15210106
- Siah2 regulates stability of prolyl-hydroxylases, controls HIF1alpha abundance, and modulates physiological responses to hypoxia. | Nakayama, K., et al. 2004. Cell. 117: 941-52. PMID: 15210114
- Glycogen synthase kinase 3beta modulates synphilin-1 ubiquitylation and cellular inclusion formation by SIAH: implications for proteasomal function and Lewy body formation. | Avraham, E., et al. 2005. J Biol Chem. 280: 42877-86. PMID: 16174773
- Cycloheximide treatment to identify components of the transitional transcriptome in PACAP-induced PC12 cell differentiation. | Ravni, A., et al. 2006. J Neurochem. 98: 1229-41. PMID: 16787409
- Isolation of Xenopus HIF-prolyl 4-hydroxylase and rescue of a small-eye phenotype caused by Siah2 over-expression. | Imaoka, S., et al. 2007. Biochem Biophys Res Commun. 355: 419-25. PMID: 17303083
- Expression of Siah-2, a vertebrate homologue of Drosophila sina, in germ cells of the mouse ovary and testis. | Della, NG., et al. 1995. Cell Tissue Res. 279: 411-9. PMID: 7895278
Ordering Information
| Product Name | Catalog # | UNIT | Price | Qty | FAVORITES | |
SIAH-2 Antibody (24E6H3) | sc-81787 | 200 µg/ml | $322.00 | |||
SIAH-2 Antibody (24E6H3): m-IgG Fc BP-HRP Bundle | sc-525735 | 200 µg Ab; 10 µg BP | $361.00 | |||
SIAH-2 Antibody (24E6H3): m-IgG2a BP-HRP Bundle | sc-546387 | 200 µg Ab; 10 µg BP | $361.00 | |||
SIAH-2 Antibody (24E6H3) AC | sc-81787 AC | 500 µg/ml, 25% agarose | $424.00 | |||
SIAH-2 Antibody (24E6H3) HRP | sc-81787 HRP | 200 µg/ml | $322.00 | |||
SIAH-2 Antibody (24E6H3) FITC | sc-81787 FITC | 200 µg/ml | $336.00 | |||
SIAH-2 Antibody (24E6H3) PE | sc-81787 PE | 200 µg/ml | $349.00 | |||
SIAH-2 Antibody (24E6H3) Alexa Fluor® 488 | sc-81787 AF488 | 200 µg/ml | $364.00 | |||
SIAH-2 Antibody (24E6H3) Alexa Fluor® 546 | sc-81787 AF546 | 200 µg/ml | $364.00 | |||
SIAH-2 Antibody (24E6H3) Alexa Fluor® 594 | sc-81787 AF594 | 200 µg/ml | $364.00 | |||
SIAH-2 Antibody (24E6H3) Alexa Fluor® 647 | sc-81787 AF647 | 200 µg/ml | $364.00 | |||
SIAH-2 Antibody (24E6H3) Alexa Fluor® 680 | sc-81787 AF680 | 200 µg/ml | $364.00 | |||
SIAH-2 Antibody (24E6H3) Alexa Fluor® 790 | sc-81787 AF790 | 200 µg/ml | $364.00 |