shrew-1 CRISPR/Cas9 KO Plasmid (h): sc-406228
- Target species: human
- 20 µg of transfection-ready, purified plasmid DNA; Suitable for up to 20 transfections
- shrew-1 CRISPR/Cas9 Knockout (KO) Plasmid (h) is a pool of plasmids, each encoding Cas9 nuclease and a target-specific 20 nt guide RNA (gRNA) designed for maximum knockout efficiency using sequences derived from the GeCKO v2 library
- gRNA sequences direct Cas9 to induce site-specific double-strand breaks (DSBs) in the shrew-1 genomic locus, resulting in gene knockout through non-homologous end joining (NHEJ)
- The puromycin resistance and RFP genes are flanked by LoxP sites, enabling removal of selection markers via Cre recombinase (Cre Vector: sc-418923) after establishing stable knockout cell lines
Ordering Information
| Product Name | Catalog # | UNIT | Price | Qty | FAVORITES | |
shrew-1 CRISPR/Cas9 KO Plasmid (h) | sc-406228 | 20 µg | $397.00 |
Overview
AJAP1 encodes shrew-1, a single-pass transmembrane protein implicated in cell–cell and cell–matrix interactions at the plasma membrane and in epithelial polarity. Reported functions link shrew-1 to modulation of cytoskeletal organization, membrane trafficking, and adhesion-associated signaling pathways that influence cell migration and tissue architecture. In human biology, altered AJAP1 expression has been associated with changes in invasive behavior and remodeling of adherens junction dynamics, supporting its use as a marker of adhesion-state transitions. These features make AJAP1/shrew-1 relevant for mechanistic studies of tumor suppressive networks, metastasis-related phenotypes, and microenvironment-dependent signaling.
shrew-1 CRISPR/Cas9 KO Plasmid (h) is a pool of plasmids designed for targeted disruption of the AJAP1 gene in human cell lines. Each plasmid co-expresses a unique single guide RNA (sgRNA) targeting a distinct site within the AJAP1 together with the Streptococcus pyogenes Cas9 nuclease. The plasmids also encode GFP, allowing fluorescent identification and enrichment of successfully transfected cells by fluorescence microscopy or flow cytometry.
The multi-guide design increases the likelihood of generating insertions or deletions (indels) that disrupt the AJAP1 open reading frame following Cas9-mediated double-strand break formation. DNA breaks introduced by the CRISPR/Cas9 system are repaired through endogenous non-homologous end joining (NHEJ) pathways, frequently resulting in frameshift mutations that abolish shrew-1 protein expression.
This CRISPR knockout system enables efficient generation of AJAP1-deficient cell models for investigation of shrew-1 signaling, functional genomics studies, cancer biology research, and evaluation of therapeutic responses in human cell lines.
Key Features
- sgRNAs targeting AJAP1 exon(s) critical for shrew-1 function
- Co-expression of SpCas9 and sgRNA from a single plasmid for simplified delivery
- GFP reporter for identification of transfected cells
- Pool of plasmids targeting multiple AJAP1 genomic sites to improve knockout efficiency
- Compatible with delivery by transfection
Design Variants
CRISPRs +/- HDRs
- gRNAs encoded by shrew-1 CRISPR/Cas9 KO Plasmid (h) and shrew-1 CRISPR/Cas9 KO Plasmid (h2) target distinct sites within the AJAP1 locus. One or both targeting designs may be available. See Related Products for availability.
- HDR donor constructs encoded by shrew-1 HDR Plasmid (h) and shrew-1 HDR Plasmid (h2) contain a puromycin resistance cassette and an RFP reporter flanked by AJAP1 homology arms to support homology-directed repair at defined AJAP1 target sites corresponding to the CRISPR/Cas9 KO designs. HDR donor availability may vary. See Related Products for availability.
For Research Use Only. Not Intended for Diagnostic or Therapeutic Use.