Anti-SHKBP1 Antibody (B-7) recommended for detection of SHKBP1 of mouse, rat and human origin by WB, IP, IF and ELISA

Anti-SHKBP1 Antibody (B-7): sc-390121

Anti-SHKBP1 Antibody (B-7) is rated 5.0 out of 5 by 1.
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Datasheets

    • Anti-SHKBP1 Antibody (B-7) is a mouse monoclonal IgG1 (kappa light chain) SHKBP1 antibody provided at 200 µg/ml
    • raised against amino acids 134-200 mapping within an internal region of SHKBP1 of human origin
    • Anti-SHKBP1 Antibody (B-7) is recommended for detection of SHKBP1 of mouse, rat and human origin by WB, IP, IF and ELISA
    • Anti-SHKBP1 Antibody (B-7) is available conjugated to agarose for IP; HRP for WB, IHC(P) and ELISA; and to either phycoerythrin or FITC for IF, IHC(P) and FCM
    • also available conjugated to Alexa Fluor® 488, Alexa Fluor® 546, Alexa Fluor® 594 or Alexa Fluor® 647 for WB (RGB), IF, IHC(P) and FCM, and for use with RGB fluorescent imaging systems, such as iBright™ FL1000, FluorChem™, Typhoon, Azure and other comparable systems
    • also available conjugated to Alexa Fluor® 680 or Alexa Fluor® 790 for WB (NIR), IF and FCM; for use with Near-Infrared (NIR) detection systems, such as LI-COR®Odyssey®, iBright™ FL1000, FluorChem™, Typhoon, Azure and other comparable systems
    • Contact our Technical Service Department (or your local Distributor) for more information on how to receive a FREE 10 µg sample of SHKBP1 (B-7): sc-390121.
    • m-IgG Fc BP-HRP and m-IgG1 BP-HRP are the preferred secondary detection reagents for SHKBP1 Antibody (B-7) for WB applications. These reagents are now offered in bundles with SHKBP1 Antibody (B-7) (see ordering information below).
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What blocking solution should I use with SHKBP1 (B-7): sc-390121 monoclonal antibody for Western blot?

Asked by: Professor Griffin
Thank you for your question. We recommend blocking with Blotto (either TBS Blotto A: sc-2333 or TBS Blotto B: sc-2335). Our Western blot protocol can be found here: https://www.scbt.com/scbt/resources/protocols/western-immuno-blotting
Answered by: Technical Support
Date published: 2017-03-01
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Rated 5 out of 5 by from Produced positive Western blot data of SHKBP1 Produced positive Western blot data of SHKBP1 expression in U-251 MG, H4, IMR-32 and SK-N-MC whole cell lysates. -SCBT QC
Date published: 2013-08-21
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