Shb/Shf Antibody (B-1): sc-74483
- Shb/Shf Antibody (B-1) is a mouse monoclonal IgG1 κ Shb/Shf antibody, cited in 1 publications, provided at 200 µg/ml
- raised against amino acids 467-596 mapping at the C-terminus of Shb of human origin
- Shb/Shf Antibody (B-1) is recommended for detection of Shb and Shf of mouse, rat and human origin by WB, IP, IF and ELISA
- Anti-Shb/Shf Antibody (B-1) is available conjugated to agarose for IP; HRP for WB, IHC(P) and ELISA; and to either phycoerythrin or FITC for IF, IHC(P) and FCM
- also available conjugated to Alexa Fluor® 488, Alexa Fluor® 546, Alexa Fluor® 594 or Alexa Fluor® 647 for WB (RGB), IF, IHC(P) and FCM, and for use with RGB fluorescent imaging systems, such as iBright™ FL1000, FluorChem™, Typhoon, Azure and other comparable systems
- also available conjugated to Alexa Fluor® 680 or Alexa Fluor® 790 for WB (NIR), IF and FCM; for use with Near-Infrared (NIR) detection systems, such as LI-COR®Odyssey®, iBright™ FL1000, FluorChem™, Typhoon, Azure and other comparable systems
- m-IgG Fc BP-HRP and m-IgG1 BP-HRP are the preferred secondary detection reagents for Shb/Shf Antibody (B-1) for WB applications. These reagents are now offered in bundles with Shb/Shf Antibody (B-1) (see ordering information below).
Shb/Shf Antibody (B-1) is a mouse monoclonal IgG1 kappa light chain antibody that detects Shb/Shf protein of mouse, rat, and human origin by western blotting (WB), immunoprecipitation (IP), immunofluorescence (IF), and enzyme-linked immunosorbent assay (ELISA). anti-Shb/Shf antibody (B-1) is available in both non-conjugated and various conjugated forms, including agarose, horseradish peroxidase (HRP), phycoerythrin (PE), fluorescein isothiocyanate (FITC), and multiple Alexa Fluor® conjugates. Shb protein, a member of the SH2 domain-containing adapter protein family, plays a crucial role in cellular signaling by linking activated receptors to downstream signaling pathways, thereby influencing gene expression and cellular responses. SH2 domain, which is a key structural feature of Shb, consists of two alpha helices and seven beta strands, allowing specific binding to tyrosine-phosphorylated proteins. This interaction is vital for propagating signaling cascades that regulate various cellular functions, including growth, differentiation, and apoptosis. Shb is predominantly localized to the cell membrane and cytoplasm, facilitating a role in signal transduction, while Shd and Shf family members function as adaptor proteins with distinct regulatory roles, including potential involvement in apoptotic processes. Shb/Shf (B-1) monoclonal antibody′s versatility in various applications makes this reagent an essential tool for researchers studying cellular signaling mechanisms and functional dynamics of SH2 domain-containing proteins.
Alexa Fluor® is a trademark of Molecular Probes Inc., OR., USA
LI-COR® and Odyssey® are registered trademarks of LI-COR Biosciences
Shb/Shf Antibody (B-1) References:
- Platelet-derived growth factor-mediated signaling through the Shb adaptor protein: effects on cytoskeletal organization. | Hooshmand-Rad, R., et al. 2000. Exp Cell Res. 257: 245-54. PMID: 10837138
- Shf, a Shb-like adapter protein, is involved in PDGF-alpha-receptor regulation of apoptosis. | Lindholm, CK., et al. 2000. Biochem Biophys Res Commun. 278: 537-43. PMID: 11095946
- Conservation analysis and structure prediction of the SH2 family of phosphotyrosine binding domains. | Russell, RB., et al. 1992. FEBS Lett. 304: 15-20. PMID: 1377638
- Shb promotes blood vessel formation in embryoid bodies by augmenting vascular endothelial growth factor receptor-2 and platelet-derived growth factor receptor-beta signaling. | Rolny, C., et al. 2005. Exp Cell Res. 308: 381-93. PMID: 15919073
- A noncatalytic domain conserved among cytoplasmic protein-tyrosine kinases modifies the kinase function and transforming activity of Fujinami sarcoma virus P130gag-fps. | Sadowski, I., et al. 1986. Mol Cell Biol. 6: 4396-408. PMID: 3025655
- Molecular interactions of the Src homology 2 domain protein Shb with phosphotyrosine residues, tyrosine kinase receptors and Src homology 3 domain proteins. | Karlsson, T., et al. 1995. Oncogene. 10: 1475-83. PMID: 7537362
- Shb is a ubiquitously expressed Src homology 2 protein. | Welsh, M., et al. 1994. Oncogene. 9: 19-27. PMID: 8302579
- Apoptosis of NIH3T3 cells overexpressing the Src homology 2 domain protein Shb. | Karlsson, T. and Welsh, M. 1996. Oncogene. 13: 955-61. PMID: 8806685
- The Src homology 2 domain protein Shb transmits basic fibroblast growth factor- and nerve growth factor-dependent differentiation signals in PC12 cells. | Karlsson, T., et al. 1998. Cell Growth Differ. 9: 757-66. PMID: 9751119
Ordering Information
| Product Name | Catalog # | UNIT | Price | Qty | FAVORITES | |
Shb/Shf Antibody (B-1) | sc-74483 | 200 µg/ml | $316.00 | |||
Shb/Shf Antibody (B-1): m-IgG Fc BP-HRP Bundle | sc-527027 | 200 µg Ab; 10 µg BP | $354.00 | |||
Shb/Shf Antibody (B-1): m-IgG1 BP-HRP Bundle | sc-532400 | 200 µg Ab; 20 µg BP | $354.00 | |||
Shb/Shf Antibody (B-1) AC | sc-74483 AC | 500 µg/ml, 25% agarose | $416.00 | |||
Shb/Shf Antibody (B-1) HRP | sc-74483 HRP | 200 µg/ml | $316.00 | |||
Shb/Shf Antibody (B-1) FITC | sc-74483 FITC | 200 µg/ml | $330.00 | |||
Shb/Shf Antibody (B-1) PE | sc-74483 PE | 200 µg/ml | $343.00 | |||
Shb/Shf Antibody (B-1) Alexa Fluor® 488 | sc-74483 AF488 | 200 µg/ml | $357.00 | |||
Shb/Shf Antibody (B-1) Alexa Fluor® 546 | sc-74483 AF546 | 200 µg/ml | $357.00 | |||
Shb/Shf Antibody (B-1) Alexa Fluor® 594 | sc-74483 AF594 | 200 µg/ml | $357.00 | |||
Shb/Shf Antibody (B-1) Alexa Fluor® 647 | sc-74483 AF647 | 200 µg/ml | $357.00 | |||
Shb/Shf Antibody (B-1) Alexa Fluor® 680 | sc-74483 AF680 | 200 µg/ml | $357.00 | |||
Shb/Shf Antibody (B-1) Alexa Fluor® 790 | sc-74483 AF790 | 200 µg/ml | $357.00 |