Sg III CRISPR/Cas9 KO Plasmid (m): sc-422812
- Target species: mouse
- 20 µg of transfection-ready, purified plasmid DNA; Suitable for up to 20 transfections
- Sg III CRISPR/Cas9 Knockout (KO) Plasmid (m) is a pool of plasmids, each encoding Cas9 nuclease and a target-specific 20 nt guide RNA (gRNA) designed for maximum knockout efficiency using sequences derived from the GeCKO v2 library
- gRNA sequences direct Cas9 to induce site-specific double-strand breaks (DSBs) in the Sg III genomic locus, resulting in gene knockout through non-homologous end joining (NHEJ)
- The puromycin resistance and RFP genes are flanked by LoxP sites, enabling removal of selection markers via Cre recombinase (Cre Vector: sc-418923) after establishing stable knockout cell lines
Ordering Information
| Product Name | Catalog # | UNIT | Price | Qty | FAVORITES | |
Sg III CRISPR/Cas9 KO Plasmid (m) | sc-422812 | 20 µg | $397.00 |
Overview
Secretogranin III (Scg3; Sg III) is a granin family protein enriched in the regulated secretory pathway, where it contributes to secretory granule biogenesis, cargo sorting, and peptide hormone/neuropeptide packaging in endocrine and neuroendocrine cells. In mouse, Sg III participates in dense-core vesicle formation and regulated exocytosis, influencing vesicular trafficking and stimulus-coupled secretion programs. Altered granin network function has been linked to dysregulated neuroendocrine signaling and metabolic homeostasis, making Scg3 a useful target for studying secretory pathway organization and stimulus-dependent release dynamics. Experimental interrogation of Scg3 supports mechanistic work on vesicle maturation, proteolytic processing of prohormones, and stress-responsive secretory remodeling.
Sg III CRISPR/Cas9 KO Plasmid (m) is a pool of plasmids designed for targeted disruption of the Scg3 gene in mouse cell lines. Each plasmid co-expresses a unique single guide RNA (sgRNA) targeting a distinct site within the Scg3 together with the Streptococcus pyogenes Cas9 nuclease. The plasmids also encode GFP, allowing fluorescent identification and enrichment of successfully transfected cells by fluorescence microscopy or flow cytometry.
The multi-guide design increases the likelihood of generating insertions or deletions (indels) that disrupt the Scg3 open reading frame following Cas9-mediated double-strand break formation. DNA breaks introduced by the CRISPR/Cas9 system are repaired through endogenous non-homologous end joining (NHEJ) pathways, frequently resulting in frameshift mutations that abolish Sg III protein expression.
This CRISPR knockout system enables efficient generation of Scg3-deficient cell models for investigation of Sg III signaling, functional genomics studies, cancer biology research, and evaluation of therapeutic responses in human cell lines.
Key Features
- sgRNAs targeting Scg3 exon(s) critical for Sg III function
- Co-expression of SpCas9 and sgRNA from a single plasmid for simplified delivery
- GFP reporter for identification of transfected cells
- Pool of plasmids targeting multiple Scg3 genomic sites to improve knockout efficiency
- Compatible with delivery by transfection
Design Variants
CRISPRs +/- HDRs
- gRNAs encoded by Sg III CRISPR/Cas9 KO Plasmid (m) and Sg III CRISPR/Cas9 KO Plasmid (m2) target distinct sites within the Scg3 locus. One or both targeting designs may be available. See Related Products for availability.
- HDR donor constructs encoded by Sg III HDR Plasmid (m) and Sg III HDR Plasmid (m2) contain a puromycin resistance cassette and an RFP reporter flanked by Scg3 homology arms to support homology-directed repair at defined Scg3 target sites corresponding to the CRISPR/Cas9 KO designs. HDR donor availability may vary. See Related Products for availability.
For Research Use Only. Not Intended for Diagnostic or Therapeutic Use.