SF4 Antibody (B-3): sc-393016
- SF4 Antibody (B-3) is a mouse monoclonal IgG1 κ SF4 antibody provided at 200 µg/ml
- specific for an epitope mapping between amino acids 5-33 at the N-terminus of SF4 of human origin
- SF4 Antibody (B-3) is recommended for detection of SF4 of mouse, rat and human origin by WB, IP, IF, IHC(P) and ELISA; also reactive with additional species, including and equine, canine and bovine
- Anti-SF4 Antibody (B-3) is available conjugated to agarose for IP; HRP for WB, IHC(P) and ELISA; and to either phycoerythrin or FITC for IF, IHC(P) and FCM
- also available conjugated to Alexa Fluor® 488, Alexa Fluor® 546, Alexa Fluor® 594 or Alexa Fluor® 647 for WB (RGB), IF, IHC(P) and FCM, and for use with RGB fluorescent imaging systems, such as iBright™ FL1000, FluorChem™, Typhoon, Azure and other comparable systems
- also available conjugated to Alexa Fluor® 680 or Alexa Fluor® 790 for WB (NIR), IF and FCM; for use with Near-Infrared (NIR) detection systems, such as LI-COR®Odyssey®, iBright™ FL1000, FluorChem™, Typhoon, Azure and other comparable systems
- m-IgG1 BP-HRP and m-IgGκ BP-HRP are the preferred secondary detection reagents for SF4 Antibody (B-3) for WB and IHC(P) applications. These reagents are now offered in bundles with SF4 Antibody (B-3) (see ordering information below).
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SEE ALSO...
SF4 Antibody (B-3) is a mouse monoclonal IgG1 kappa light chain antibody that detects SF4 of mouse, rat, and human origin by western blotting (WB), immunoprecipitation (IP), immunofluorescence (IF), immunohistochemistry, and enzyme-linked immunosorbent assay (ELISA). SF4 (B-3) antibody is available in both non-conjugated and various conjugated forms, including agarose, horseradish peroxidase (HRP), phycoerythrin (PE), fluorescein isothiocyanate (FITC), and multiple Alexa Fluor® conjugates. SF4, also known as splicing factor 4 or RNA-binding protein, is a crucial 645 amino acid nuclear protein that plays a significant role in pre-mRNA splicing, a vital process for gene expression regulation. SF4 is predominantly expressed in the testis and heart, but is also present in skeletal muscle, brain, and kidney, where SF4 acts as an essential component of the spliceosome, the molecular machinery responsible for removing introns from pre-mRNA. The structure of SF4 includes two SURP motif repeats and one G-patch domain, which are critical for RNA processing function. The gene encoding SF4 is located on human chromosome 19p13.11, a region known for high gene density and housing numerous immunoglobulin superfamily members, which are important for immune response. Understanding SF4 structure and function is vital, as alterations in splicing mechanisms can lead to various diseases, including cancer and genetic disorders, highlighting anti-SF4 antibody (B-3)′s importance in research and diagnostic applications.
Alexa Fluor® is a trademark of Molecular Probes Inc., OR., USA
LI-COR® and Odyssey® are registered trademarks of LI-COR Biosciences
SF4 Antibody (B-3) References:
- C-CAM1, a candidate tumor suppressor gene, is abnormally expressed in primary lung cancers. | Wang, L., et al. 2000. Clin Cancer Res. 6: 2988-93. PMID: 10955775
- The genomic context of natural killer receptor extended gene families. | Trowsdale, J., et al. 2001. Immunol Rev. 181: 20-38. PMID: 11513141
- SF4 and SFRS14, two related putative splicing factors on human chromosome 19p13.11. | Sampson, ND. and Hewitt, JE. 2003. Gene. 305: 91-100. PMID: 12594045
- Comparative human-mouse-rat sequence analysis of the ICAM gene cluster on HSA 19p13.2 and a 185-kb porcine region from SSC 2q. | Leeb, T. and Müller, M. 2004. Gene. 343: 239-44. PMID: 15588578
- The extended human leukocyte receptor complex: diverse ways of modulating immune responses. | Barrow, AD. and Trowsdale, J. 2008. Immunol Rev. 224: 98-123. PMID: 18759923
- Gene organization of the pregnancy-specific glycoprotein region on human chromosome 19: assembly and analysis of a 700-kb cosmid contig spanning the region. | Olsen, A., et al. 1994. Genomics. 23: 659-68. PMID: 7851895
- The pregnancy-specific glycoprotein (PSG) gene cluster on human chromosome 19: fine structure of the 11 PSG genes and identification of 6 new genes forming a third subgroup within the carcinoembryonic antigen (CEA) family. | Teglund, S., et al. 1994. Genomics. 23: 669-84. PMID: 7851896
Ordering Information
| Product Name | Catalog # | UNIT | Price | Qty | FAVORITES | |
SF4 Antibody (B-3) | sc-393016 | 200 µg/ml | $322.00 | |||
SF4 Antibody (B-3): m-IgGκ BP-HRP Bundle | sc-523777 | 200 µg Ab, 40 µg BP | $361.00 | |||
SF4 Antibody (B-3): m-IgG1 BP-HRP Bundle | sc-533609 | 200 µg Ab; 20 µg BP | $361.00 | |||
SF4 Antibody (B-3) AC | sc-393016 AC | 500 µg/ml, 25% agarose | $424.00 | |||
SF4 Antibody (B-3) HRP | sc-393016 HRP | 200 µg/ml | $322.00 | |||
SF4 Antibody (B-3) FITC | sc-393016 FITC | 200 µg/ml | $336.00 | |||
SF4 Antibody (B-3) PE | sc-393016 PE | 200 µg/ml | $349.00 | |||
SF4 Antibody (B-3) Alexa Fluor® 488 | sc-393016 AF488 | 200 µg/ml | $364.00 | |||
SF4 Antibody (B-3) Alexa Fluor® 546 | sc-393016 AF546 | 200 µg/ml | $364.00 | |||
SF4 Antibody (B-3) Alexa Fluor® 594 | sc-393016 AF594 | 200 µg/ml | $364.00 | |||
SF4 Antibody (B-3) Alexa Fluor® 647 | sc-393016 AF647 | 200 µg/ml | $364.00 | |||
SF4 Antibody (B-3) Alexa Fluor® 680 | sc-393016 AF680 | 200 µg/ml | $364.00 | |||
SF4 Antibody (B-3) Alexa Fluor® 790 | sc-393016 AF790 | 200 µg/ml | $364.00 | |||
SF4 (B-3) Neutralizing Peptide | sc-393016 P | 100 µg/0.5 ml | $69.00 |