Not CRISPR data: the image illustrates antibody quality for visualizing CRISPR experiment results.
Not CRISPR data: the image illustrates antibody quality for visualizing CRISPR experiment results.
SerpinB6 CRISPR/Cas9 KO Plasmid (h): sc-417434
- Target species: human
- 20 µg of transfection-ready, purified plasmid DNA; Suitable for up to 20 transfections
- SerpinB6 CRISPR/Cas9 Knockout (KO) Plasmid (h) is a pool of plasmids, each encoding Cas9 nuclease and a target-specific 20 nt guide RNA (gRNA) designed for maximum knockout efficiency using sequences derived from the GeCKO v2 library
- gRNA sequences direct Cas9 to induce site-specific double-strand breaks (DSBs) in the SerpinB6 genomic locus, resulting in gene knockout through non-homologous end joining (NHEJ)
- The puromycin resistance and RFP genes are flanked by LoxP sites, enabling removal of selection markers via Cre recombinase (Cre Vector: sc-418923) after establishing stable knockout cell lines
- Following transfection, gene knockout efficiency can be assayed by WB, IF or IHC using antibody: SerpinB6 Antibody (E-8): sc-398487
Ordering Information
| Product Name | Catalog # | UNIT | Price | Qty | FAVORITES | |
SerpinB6 CRISPR/Cas9 KO Plasmid (h) | sc-417434 | 20 µg | $397.00 |
Overview
SERPINB6 encodes SerpinB6, an intracellular clade B serine protease inhibitor that contributes to cytoprotection by neutralizing aberrant protease activity and limiting proteolysis-driven cellular damage. By constraining serine proteases within the cytosol and nucleus, SerpinB6 supports proteostasis and stress-response processes that influence cell survival, differentiation, and inflammatory signaling. SERPINB6 expression is particularly relevant in epithelial and immune contexts where protease–antiprotease balance shapes barrier function and tissue homeostasis. Dysregulated serpin activity has been associated with altered susceptibility to proteotoxic stress and inflammatory pathology, making SERPINB6 a useful target for mechanistic studies of intracellular protease control.
SerpinB6 CRISPR/Cas9 KO Plasmid (h) is a pool of plasmids designed for targeted disruption of the SERPINB6 gene in human cell lines. Each plasmid co-expresses a unique single guide RNA (sgRNA) targeting a distinct site within the SERPINB6 together with the Streptococcus pyogenes Cas9 nuclease. The plasmids also encode GFP, allowing fluorescent identification and enrichment of successfully transfected cells by fluorescence microscopy or flow cytometry.
The multi-guide design increases the likelihood of generating insertions or deletions (indels) that disrupt the SERPINB6 open reading frame following Cas9-mediated double-strand break formation. DNA breaks introduced by the CRISPR/Cas9 system are repaired through endogenous non-homologous end joining (NHEJ) pathways, frequently resulting in frameshift mutations that abolish SerpinB6 protein expression.
This CRISPR knockout system enables efficient generation of SERPINB6-deficient cell models for investigation of SerpinB6 signaling, functional genomics studies, cancer biology research, and evaluation of therapeutic responses in human cell lines.
Key Features
- sgRNAs targeting SERPINB6 exon(s) critical for SerpinB6 function
- Co-expression of SpCas9 and sgRNA from a single plasmid for simplified delivery
- GFP reporter for identification of transfected cells
- Pool of plasmids targeting multiple SERPINB6 genomic sites to improve knockout efficiency
- Compatible with delivery by transfection
Design Variants
CRISPRs +/- HDRs
- gRNAs encoded by SerpinB6 CRISPR/Cas9 KO Plasmid (h) and SerpinB6 CRISPR/Cas9 KO Plasmid (h2) target distinct sites within the SERPINB6 locus. One or both targeting designs may be available. See Related Products for availability.
- HDR donor constructs encoded by SerpinB6 HDR Plasmid (h) and SerpinB6 HDR Plasmid (h2) contain a puromycin resistance cassette and an RFP reporter flanked by SERPINB6 homology arms to support homology-directed repair at defined SERPINB6 target sites corresponding to the CRISPR/Cas9 KO designs. HDR donor availability may vary. See Related Products for availability.
For Research Use Only. Not Intended for Diagnostic or Therapeutic Use.