SerpinB6 Antibody (F-5): sc-374535
- SerpinB6 Antibody (F-5) is a mouse monoclonal IgG2b κ provided at 200 µg/ml
- specific for an epitope mapping between amino acids 273-303 within an internal region of SerpinB6 of mouse origin
- Anti-SerpinB6 Antibody (F-5) is recommended for detection of SerpinB6 of mouse and rat origin by WB, IP, IF, IHC(P) and ELISA
- Anti-SerpinB6 Antibody (F-5) is available conjugated to agarose for IP; HRP for WB, IHC(P) and ELISA; and to either phycoerythrin or FITC for IF, IHC(P) and FCM
- also available conjugated to Alexa Fluor® 488, Alexa Fluor® 546, Alexa Fluor® 594 or Alexa Fluor® 647 for WB (RGB), IF, IHC(P) and FCM, and for use with RGB fluorescent imaging systems, such as iBright™ FL1000, FluorChem™, Typhoon, Azure and other comparable systems
- also available conjugated to Alexa Fluor® 680 or Alexa Fluor® 790 for WB (NIR), IF and FCM; for use with Near-Infrared (NIR) detection systems, such as LI-COR®Odyssey®, iBright™ FL1000, FluorChem™, Typhoon, Azure and other comparable systems
- m-IgG2b BP-HRP is the preferred secondary detection reagent for SerpinB6 Antibody (F-5) for WB and IHC(P) applications. This reagent is now offered in a bundle with SerpinB6 Antibody (F-5) (see ordering information below).
SerpinB6 Antibody (F-5) is a mouse monoclonal IgG2b kappa light chain antibody that detects SerpinB6 in mouse and rat samples through applications such as western blotting (WB), immunoprecipitation (IP), immunofluorescence (IF), immunohistochemistry, and enzyme-linked immunosorbent assay (ELISA). Anti-SerpinB6 antibody (F-5) is available in both non-conjugated and various conjugated forms, including agarose, horseradish peroxidase (HRP), phycoerythrin (PE), fluorescein isothiocyanate (FITC), and multiple Alexa Fluor® conjugates. SerpinB6, also known as protease inhibitor 6 (PI-6) in humans and Spi3 in mice, plays a crucial role as a serine protease inhibitor, exhibiting both antitrypsin and antichymotrypsin activities. SerpinB6 is primarily located in the cytoplasm, allowing effective regulation of intracellular proteolytic processes, distinguishing SerpinB6 from many other serpins that are secreted and function extracellularly. Cytoplasmic presence enables SerpinB6 interactions with various proteases, including arginine-selective protease human kallikrein 2 (hK2) and azurophilic granule protease cathepsin G found in monocytes and granulocytes. SerpinB6 forms stable complexes with these proteases, highlighting SerpinB6′s importance in modulating inflammatory responses and maintaining cellular homeostasis. In mice, the SerpinB6 gene family consists of three distinct genes: SerpinB6a, SerpinB6b, and SerpinB6c, each contributing to diverse regulatory functions in various tissues, including capillary endothelial cells, platelets, and epithelial cells.
Alexa Fluor® is a trademark of Molecular Probes Inc., OR., USA
LI-COR® and Odyssey® are registered trademarks of LI-COR Biosciences
SerpinB6 Antibody (F-5) References:
- The intracellular serpin proteinase inhibitor 6 is expressed in monocytes and granulocytes and is a potent inhibitor of the azurophilic granule protease, cathepsin G. | Scott, FL., et al. 1999. Blood. 93: 2089-97. PMID: 10068683
- Identification of a novel complex between human kallikrein 2 and protease inhibitor-6 in prostate cancer tissue. | Mikolajczyk, SD., et al. 1999. Cancer Res. 59: 3927-30. PMID: 10463585
- Nucleocytoplasmic distribution of the ovalbumin serpin PI-9 requires a nonconventional nuclear import pathway and the export factor Crm1. | Bird, CH., et al. 2001. Mol Cell Biol. 21: 5396-407. PMID: 11463822
- Human kallikrein 2 (hK2), but not prostate-specific antigen (PSA), rapidly complexes with protease inhibitor 6 (PI-6) released from prostate carcinoma cells. | Saedi, MS., et al. 2001. Int J Cancer. 94: 558-63. PMID: 11745444
- Gene structure, chromosomal localization, and expression of the murine homologue of human proteinase inhibitor 6 (PI-6) suggests divergence of PI-6 from the ovalbumin serpins. | Sun, J., et al. 1995. J Biol Chem. 270: 16089-96. PMID: 7608171
- Proteinase inhibitor 6 cannot be secreted, which suggests it is a new type of cellular serpin. | Scott, FL., et al. 1996. J Biol Chem. 271: 1605-12. PMID: 8576159
Ordering Information
| Product Name | Catalog # | UNIT | Price | Qty | FAVORITES | |
SerpinB6 Antibody (F-5) | sc-374535 | 200 µg/ml | $322.00 | |||
SerpinB6 Antibody (F-5): m-IgG2b BP-HRP Bundle | sc-548746 | 200 µg Ab; 10 µg BP | $361.00 | |||
SerpinB6 Antibody (F-5) AC | sc-374535 AC | 500 µg/ml, 25% agarose | $424.00 | |||
SerpinB6 Antibody (F-5) HRP | sc-374535 HRP | 200 µg/ml | $322.00 | |||
SerpinB6 Antibody (F-5) FITC | sc-374535 FITC | 200 µg/ml | $336.00 | |||
SerpinB6 Antibody (F-5) PE | sc-374535 PE | 200 µg/ml | $349.00 | |||
SerpinB6 Antibody (F-5) Alexa Fluor® 488 | sc-374535 AF488 | 200 µg/ml | $364.00 | |||
SerpinB6 Antibody (F-5) Alexa Fluor® 546 | sc-374535 AF546 | 200 µg/ml | $364.00 | |||
SerpinB6 Antibody (F-5) Alexa Fluor® 594 | sc-374535 AF594 | 200 µg/ml | $364.00 | |||
SerpinB6 Antibody (F-5) Alexa Fluor® 647 | sc-374535 AF647 | 200 µg/ml | $364.00 | |||
SerpinB6 Antibody (F-5) Alexa Fluor® 680 | sc-374535 AF680 | 200 µg/ml | $364.00 | |||
SerpinB6 Antibody (F-5) Alexa Fluor® 790 | sc-374535 AF790 | 200 µg/ml | $364.00 | |||
SerpinB6 (F-5) Neutralizing Peptide | sc-374535 P | 100 µg/0.5 ml | $69.00 |