Not CRISPR data: the image illustrates antibody quality for visualizing CRISPR experiment results.
Not CRISPR data: the image illustrates antibody quality for visualizing CRISPR experiment results.
SerpinB1 Double Nickase Plasmid (h): sc-402083-NIC
- Target species: human
- 20 µg of transfection-ready, purified plasmid DNA; Suitable for up to 20 transfections
- SerpinB1 Double Nickase Plasmid (h) consists of a pair of plasmids each encoding a D10A mutated Cas9 nuclease and a target-specific 20 nt guide RNA (gRNA) designed to knockout gene expression with greater specificity than its CRISPR/Cas9 KO counterpart
- Paired gRNA sequences are offset by approximately 20 bp to allow for specific Cas9-mediated double nicking of the genomic DNA, which mimics a DSB
- One plasmid in the pair contains a puromycin-resistance gene for selection; the other plasmid in the pair contains a GFP marker to visually confirm transfection
- SerpinB1 Double Nickase Plasmid (h) and SerpinB1 Double Nickase Plasmid (h2) encode distinct paired gRNA designs targeting SERPINB1. One or both designs may be available
- Following transfection, gene knockout efficiency can be assayed by WB, IF or IHC using antibody: SerpinB1 Antibody (4D7): sc-293462
Ordering Information
| Product Name | Catalog # | UNIT | Price | Qty | FAVORITES | |
SerpinB1 Double Nickase Plasmid (h) | sc-402083-NIC | 20 µg | $410.00 | |||
SerpinB1 Double Nickase Plasmid (h2) | sc-402083-NIC-2 | 20 µg | $410.00 |
SERPINB1 encodes SerpinB1, an intracellular serine protease inhibitor that restrains neutrophil-derived proteases such as elastase and cathepsin G, thereby limiting proteolytic stress and supporting tissue homeostasis. SerpinB1 contributes to regulation of inflammatory signaling, leukocyte survival, and epithelial barrier integrity by modulating protease-driven pathways linked to oxidative stress and cell death. Altered SERPINB1 expression or function has been associated with dysregulated innate immune responses and protease imbalance in inflammatory airway and skin contexts, and it is also used as a marker in studies of myeloid cell states. These features make SERPINB1 a useful target for dissecting protease-inhibitor networks, neutrophil biology, and inflammation-associated remodeling mechanisms in human cell models.
SerpinB1 Double Nickase Plasmid (h) consists of a matched pair of plasmids engineered for high-specificity editing of the SERPINB1 locus in human cell lines. Each plasmid expresses a Cas9 D10A nickase and a distinct sgRNA targeting opposite DNA strands within SERPINB1. When directed to adjacent sites on opposite DNA strands, the two nickases generate offset single-strand nicks that together produce a staggered double-strand break, requiring coordinated on-target activity from both guides. The resulting DNA break is resolved by endogenous cellular repair pathways, most commonly through non-homologous end joining (NHEJ), leading to insertions or deletions that disrupt SERPINB1 function. By requiring dual sgRNA engagement at the target locus, the double nicking approach enhances editing specificity and provides a complementary CRISPR strategy for applications where additional control over targeting precision is desired.
To support efficient identification of edited cells, one plasmid encodes GFP for fluorescent visualization of transfected populations, while the companion plasmid carries a puromycin resistance gene for antibiotic selection. Together, these features support efficient enrichment of co-transfected populations and simplify the validation of SERPINB1-disrupted clones.
For Research Use Only. Not Intended for Diagnostic or Therapeutic Use.