Serine racemase CRISPR Activation Plasmid (h): sc-402360-ACT
- Target species: human
- 20 µg of transfection-ready, purified plasmid DNA; Suitable for up to 20 transfections
- Serine racemase CRISPR Activation Plasmid (h) is a synergistic activation mediator (SAM) transcription activation system designed to specifically upregulate gene expression
- Serine racemase CRISPR Activation Plasmid (h) consists of three plasmids at a 1:1:1 mass ratio: a plasmid encoding the deactivated Cas9 (dCas9) nuclease (D10A and N863A) fused to the transactivation domain VP64, and a blasticidin resistance gene; a plasmid encoding the MS2-p65-HSF1 fusion protein, and a hygromycin resistance gene; a plasmid encoding a target-specific 20 nt guide RNA fused to two MS2 RNA aptamers, and a puromycin resistance gene
- The resulting SAM complex binds to a site-specific region approximately 200-250 nt upstream of the transcriptional start site and provides robust recruitment of transcription factors for highly efficient gene activation
- gRNAs encoded by Serine racemase CRISPR Activation Plasmid (h) and Serine racemase CRISPR Activation Plasmid (h2) target distinct regulatory regions upstream of the SRR transcriptional start site. One or both designs may be available
- Following transfection, gene knockout efficiency can be assayed by WB, IF or IHC using antibody: Serine racemase Antibody (A-4): sc-365217
Ordering Information
| Product Name | Catalog # | UNIT | Price | Qty | FAVORITES | |
Serine racemase CRISPR Activation Plasmid (h) | sc-402360-ACT | 20 µg | $397.00 |
Human SRR encodes serine racemase, a pyridoxal phosphate–dependent enzyme that catalyzes the interconversion of L-serine and D-serine, a key co-agonist of NMDA-type glutamate receptors. By regulating D-serine availability, serine racemase influences excitatory neurotransmission, synaptic plasticity, and downstream calcium-dependent signaling cascades in neuronal and glial contexts. SRR activity links amino acid metabolism to redox biology through interactions with pathways controlling oxidative stress and cellular energy balance. Altered SRR expression or D-serine homeostasis has been associated with neuropsychiatric and neurodegenerative disease mechanisms, making SRR a useful target for studies of NMDA receptor–related signaling and metabolic regulation.
Serine racemase CRISPR Activation Plasmid (h) provides a targeted, non-destructive approach to upregulating endogenous SRR expression without altering the underlying DNA sequence.
Serine racemase CRISPR Activation Plasmid (h) is a three-plasmid synergistic activation mediator (SAM) system engineered for highly efficient, site-specific transcriptional upregulation of the SRR locus in human cell lines. The system is built around a catalytically inactive Cas9 (dCas9) carrying two inactivating mutations (D10A and N863A) that eliminate nuclease activity while preserving DNA binding. This dCas9 is fused to VP64, a potent transcriptional activator, and is co-expressed with a blasticidin resistance gene for selection. The second plasmid encodes the MS2-p65-HSF1 fusion protein, a secondary activator complex that works in concert with dCas9-VP64, alongside a hygromycin resistance gene. The third plasmid encodes a target-specific 20 nt sgRNA fused to two MS2 RNA aptamers that recruit the MS2-p65-HSF1 complex to the activation site, accompanied by a puromycin resistance gene. The three plasmids are delivered at a 1:1:1 mass ratio for balanced expression of all system components.
Once assembled at the target locus, the SAM complex binds within approximately 200 bp upstream of the SRR transcriptional start site, where VP64, p65, and HSF1 act in concert to recruit transcriptional machinery and drive upregulation of endogenous Serine racemase expression. Unlike nuclease-active Cas9, dCas9 does not introduce double-strand breaks or modify the genomic sequence, preserving the native SRR locus and enabling the study of Serine racemase-dependent transcriptional responses at the endogenous locus, making it a valuable tool for functional studies, target gene identification, and the modeling of Serine racemase pathway restoration in tumor cells with silenced or reduced SRR expression.
For Research Use Only. Not Intended for Diagnostic or Therapeutic Use.