SERF1 CRISPR/Cas9 KO Plasmid (m): sc-422894
- Target species: mouse
- 20 µg of transfection-ready, purified plasmid DNA; Suitable for up to 20 transfections
- SERF1 CRISPR/Cas9 Knockout (KO) Plasmid (m) is a pool of plasmids, each encoding Cas9 nuclease and a target-specific 20 nt guide RNA (gRNA) designed for maximum knockout efficiency using sequences derived from the GeCKO v2 library
- gRNA sequences direct Cas9 to induce site-specific double-strand breaks (DSBs) in the SERF1 genomic locus, resulting in gene knockout through non-homologous end joining (NHEJ)
- The puromycin resistance and RFP genes are flanked by LoxP sites, enabling removal of selection markers via Cre recombinase (Cre Vector: sc-418923) after establishing stable knockout cell lines
Ordering Information
| Product Name | Catalog # | UNIT | Price | Qty | FAVORITES | |
SERF1 CRISPR/Cas9 KO Plasmid (m) | sc-422894 | 20 µg | $397.00 |
Overview
Serf1 encodes the small EDRK-rich factor 1 (SERF1), a highly conserved, intrinsically disordered protein implicated in proteostasis and RNA-associated processes. SERF1 has been linked to modulation of protein aggregation dynamics and cellular stress responses, with reported interactions that influence nucleolar function and RNA metabolism. In mouse systems, Serf1 is studied for its contributions to neuronal and developmental homeostasis, where altered proteostasis networks are relevant to neurodegeneration and other protein-misfolding–associated phenotypes. Loss- or gain-of-function perturbation of Serf1 supports mechanistic studies connecting chaperone pathways, ribonucleoprotein organization, and aggregation-prone proteins.
SERF1 CRISPR/Cas9 KO Plasmid (m) is a pool of plasmids designed for targeted disruption of the Serf1 gene in mouse cell lines. Each plasmid co-expresses a unique single guide RNA (sgRNA) targeting a distinct site within the Serf1 together with the Streptococcus pyogenes Cas9 nuclease. The plasmids also encode GFP, allowing fluorescent identification and enrichment of successfully transfected cells by fluorescence microscopy or flow cytometry.
The multi-guide design increases the likelihood of generating insertions or deletions (indels) that disrupt the Serf1 open reading frame following Cas9-mediated double-strand break formation. DNA breaks introduced by the CRISPR/Cas9 system are repaired through endogenous non-homologous end joining (NHEJ) pathways, frequently resulting in frameshift mutations that abolish SERF1 protein expression.
This CRISPR knockout system enables efficient generation of Serf1-deficient cell models for investigation of SERF1 signaling, functional genomics studies, cancer biology research, and evaluation of therapeutic responses in human cell lines.
Key Features
- sgRNAs targeting Serf1 exon(s) critical for SERF1 function
- Co-expression of SpCas9 and sgRNA from a single plasmid for simplified delivery
- GFP reporter for identification of transfected cells
- Pool of plasmids targeting multiple Serf1 genomic sites to improve knockout efficiency
- Compatible with delivery by transfection
Design Variants
CRISPRs +/- HDRs
- gRNAs encoded by SERF1 CRISPR/Cas9 KO Plasmid (m) and SERF1 CRISPR/Cas9 KO Plasmid (m2) target distinct sites within the Serf1 locus. One or both targeting designs may be available. See Related Products for availability.
- HDR donor constructs encoded by SERF1 HDR Plasmid (m) and SERF1 HDR Plasmid (m2) contain a puromycin resistance cassette and an RFP reporter flanked by Serf1 homology arms to support homology-directed repair at defined Serf1 target sites corresponding to the CRISPR/Cas9 KO designs. HDR donor availability may vary. See Related Products for availability.
For Research Use Only. Not Intended for Diagnostic or Therapeutic Use.