SERCA2 Double Nickase Plasmid (h): sc-400417-NIC
- Target species: human
- 20 µg of transfection-ready, purified plasmid DNA; Suitable for up to 20 transfections
- SERCA2 Double Nickase Plasmid (h) consists of a pair of plasmids each encoding a D10A mutated Cas9 nuclease and a target-specific 20 nt guide RNA (gRNA) designed to knockout gene expression with greater specificity than its CRISPR/Cas9 KO counterpart
- Paired gRNA sequences are offset by approximately 20 bp to allow for specific Cas9-mediated double nicking of the genomic DNA, which mimics a DSB
- One plasmid in the pair contains a puromycin-resistance gene for selection; the other plasmid in the pair contains a GFP marker to visually confirm transfection
- SERCA2 Double Nickase Plasmid (h) and SERCA2 Double Nickase Plasmid (h2) encode distinct paired gRNA designs targeting ATP2A2. One or both designs may be available
- Following transfection, gene knockout efficiency can be assayed by WB, IF or IHC using antibody: SERCA2 Antibody (F-1): sc-376235
Ordering Information
| Product Name | Catalog # | UNIT | Price | Qty | FAVORITES | |
SERCA2 Double Nickase Plasmid (h) | sc-400417-NIC | 20 µg | $410.00 | |||
SERCA2 Double Nickase Plasmid (h2) | sc-400417-NIC-2 | 20 µg | $410.00 |
ATP2A2 encodes the human sarco/endoplasmic reticulum Ca2+ ATPase SERCA2, a P-type ATPase that transports cytosolic Ca2+ into the ER lumen to sustain Ca2+ storage and signaling homeostasis. By shaping ER Ca2+ load, SERCA2 regulates excitation–contraction coupling, store-operated Ca2+ entry dynamics, and Ca2+-dependent control of protein folding, secretion, and cell survival pathways linked to ER stress responses. Perturbation of ATP2A2 disrupts intracellular Ca2+ oscillations and can alter downstream signaling networks including calcineurin/NFAT and unfolded protein response signaling. ATP2A2 variation is associated with Darier disease and has been implicated in contexts where ER Ca2+ handling influences cardiac and epithelial physiology, supporting mechanistic studies of Ca2+ signaling and proteostasis.
SERCA2 Double Nickase Plasmid (h) consists of a matched pair of plasmids engineered for high-specificity editing of the ATP2A2 locus in human cell lines. Each plasmid expresses a Cas9 D10A nickase and a distinct sgRNA targeting opposite DNA strands within ATP2A2. When directed to adjacent sites on opposite DNA strands, the two nickases generate offset single-strand nicks that together produce a staggered double-strand break, requiring coordinated on-target activity from both guides. The resulting DNA break is resolved by endogenous cellular repair pathways, most commonly through non-homologous end joining (NHEJ), leading to insertions or deletions that disrupt ATP2A2 function. By requiring dual sgRNA engagement at the target locus, the double nicking approach enhances editing specificity and provides a complementary CRISPR strategy for applications where additional control over targeting precision is desired.
To support efficient identification of edited cells, one plasmid encodes GFP for fluorescent visualization of transfected populations, while the companion plasmid carries a puromycin resistance gene for antibiotic selection. Together, these features support efficient enrichment of co-transfected populations and simplify the validation of ATP2A2-disrupted clones.
For Research Use Only. Not Intended for Diagnostic or Therapeutic Use.