SERCA1 Antibody (A-6): sc-515162

SERCA1 Antibody (A-6) is a mouse monoclonal IgG1 antibody that detects SERCA1 protein of mouse, rat, and human origin by western blotting (WB), immunoprecipitation (IP), immunofluorescence (IF), immunohistochemistry, and enzyme-linked immunosorbent assay (ELISA). SERCA1 (A-6) antibody is available in both non-conjugated and various conjugated forms, including agarose, horseradish peroxidase (HRP), phycoerythrin (PE), fluorescein isothiocyanate (FITC), and multiple Alexa Fluor® conjugates. SERCA1, a member of the sarcoplasmic or endoplasmic reticulum calcium ATPase family, plays a crucial role in calcium homeostasis by mediating Ca2+ uptake into the sarcoplasmic reticulum, essential for muscle contraction and relaxation. SERCA1 is predominantly expressed in type II (fast) skeletal muscle, where SERCA1 facilitates rapid muscle contraction by ensuring low cytoplasmic calcium levels, promoting muscular relaxation after contraction. Proper functioning of SERCA1 is vital for muscle performance and overall muscle health, as dysregulation of calcium handling can lead to muscle fatigue and various muscle disorders. SERCA1, along with isoforms SERCA2 and SERCA3, undergoes alternative splicing, allowing tissue-specific expression and functional diversity, further underscoring SERCA1′s importance in muscle physiology and potential implications in cardiac function and muscle-related diseases.

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SERCA1 Antibody (A-6) References:

1. SERCA1 overexpression minimizes skeletal muscle damage in dystrophic mouse models.  |  Mázala, DA., et al. 2015. Am J Physiol Cell Physiol. 308: C699-709. PMID: 25652448
2. Slowed relaxation of diaphragm in septic rats is associated with reduced expression of sarco-endoplasmic reticulum CA2+ -ATPase genes SERCA1 and SERCA2.  |  Wu, J., et al. 2016. Muscle Nerve. 54: 1108-1113. PMID: 27104787
3. Luminal Ca2+ regulation of RyR1 Ca2+ channel leak activation and inactivation in sarcoplasmic reticulum membrane vesicles.  |  Palahniuk, C., et al. 2021. Can J Physiol Pharmacol. 99: 192-206. PMID: 33161753
4. Selenoprotein K knockdown induces apoptosis in skeletal muscle satellite cells via calcium dyshomeostasis-mediated endoplasmic reticulum stress.  |  Fan, RF., et al. 2023. Poult Sci. 102: 103053. PMID: 37716231
5. SERCA1 Overexpression in Skeletal Muscle Attenuates Muscle Atrophy and Improves Motor Function in a Mouse Model of ALS.  |  Mázala, DAG., et al. 2024. J Neuromuscul Dis. 11: 315-326. PMID: 38217607
6. Relative sarcolipin (SLN) and sarcoplasmic reticulum Ca2+ ATPase (SERCA1) transcripts levels in closely related endothermic and ectothermic scombrid fishes: Implications for molecular basis of futile calcium cycle non-shivering thermogenesis (NST).  |  Robinson, S., et al. 2024. Comp Biochem Physiol A Mol Integr Physiol. 295: 111667. PMID: 38782254
7. Expression of two isoforms of the third sarco/endoplasmic reticulum Ca2+ ATPase (SERCA3) in platelets. Possible recognition of the SERCA3b isoform by the PL/IM430 monoclonal antibody.  |  Bobe, R., et al. 1998. FEBS Lett. 423: 259-64. PMID: 9512369
8. Calcium ATPase and respiratory muscle function.  |  Aubier, M. and Viires, N. 1998. Eur Respir J. 11: 758-66. PMID: 9596133
9. Characterization of the 3' end of the mouse SERCA 3 gene and tissue distribution of mRNA spliced variants.  |  Ozog, A., et al. 1998. FEBS Lett. 427: 349-52. PMID: 9637255
10. Enhanced myocardial contractility and increased Ca2+ transport function in transgenic hearts expressing the fast-twitch skeletal muscle sarcoplasmic reticulum Ca2+-ATPase.  |  Loukianov, E., et al. 1998. Circ Res. 83: 889-97. PMID: 9797337
11. Cellular distribution of Ca2+ pumps and Ca2+ release channels in rat cardiac hypertrophy induced by aortic stenosis.  |  Anger, M., et al. 1998. Circulation. 98: 2477-86. PMID: 9832495
12. Functional characterization of alternatively spliced human SERCA3 transcripts.  |  Poch, E., et al. 1998. Am J Physiol. 275: C1449-58. PMID: 9843705

Monoclonal antibodies for mammalian and non-mammalian protein targets represent essentially all targets covered by polyclonal antibodies. Over 8,300 of our antibodies are offered as ImmunoCruz^® conjugates which eliminates the need for a secondary antibody.

Santa Cruz Biotechnology antibodies have over 360,000 research citations. Anti-SERCA1 Antibody (A-6) has 4 citations in a variety of scientific publications.

Primary antibodies like Anti-SERCA1 Antibody (A-6) for mammalian target proteins are recommended for the detection of a range of mammalian species, primarily of mouse, rat and human species. Many mammalian antibodies are also ideal for veterinary research and reactive with ovine, porcine, caprine, feline, canine, bovine and equine protein targets. Santa Cruz Biotechnology primary monoclonal antibodies are suitable for laboratory research applications and are not intended for diagnostic or therapeutic use.

SERCA1 Antibody (A-6) Specifications

Alternate Product Name: SERCA1

Product Clone Name: A-6

Classification: Primary Antibody

Clonality: Monoclonal Antibody

Antibody Isotype: IgG₁ lambda light chain

Amino Acids: 851-874

Epitope Target: SERCA1

Epitope Species: human

Product Reactivity/Detection: SERCA1

Species Reactivity/Detection: mouse, rat, human

Additional Mammalian Reactivity: N/A

Additional Non-Mammalian Reactivity: N/A

Applications: WB, IP, IF, IHC(P), ELISA

Vialing Information: Each vial contains 200 µg IgG₁ lambda light chain in 1.0 ml of PBS with < 0.1% sodium azide and 0.1% gelatin

Research Citations: 4 citations in various scientific publications

Available Conjugates/Forms for SERCA1 Antibody (A-6): AC: Agarose, AF488: Alexa Fluor^® 488, AF546: Alexa Fluor^® 546, AF594: Alexa Fluor^® 594, AF647: Alexa Fluor^® 647, AF680: Alexa Fluor^® 680, AF790: Alexa Fluor^® 790, FITC: Fluoroscein, HRP: Horseradish Peroxidase, P: Blocking Peptide, PE: Phycoerythrin

The preferred SERCA1/2/3 antibody is SERCA1/2/3 Antibody (B-7): sc-271669.