Septin 9 Double Nickase Plasmid (h): sc-403987-NIC
- Target species: human
- 20 µg of transfection-ready, purified plasmid DNA; Suitable for up to 20 transfections
- Septin 9 Double Nickase Plasmid (h) consists of a pair of plasmids each encoding a D10A mutated Cas9 nuclease and a target-specific 20 nt guide RNA (gRNA) designed to knockout gene expression with greater specificity than its CRISPR/Cas9 KO counterpart
- Paired gRNA sequences are offset by approximately 20 bp to allow for specific Cas9-mediated double nicking of the genomic DNA, which mimics a DSB
- One plasmid in the pair contains a puromycin-resistance gene for selection; the other plasmid in the pair contains a GFP marker to visually confirm transfection
- Septin 9 Double Nickase Plasmid (h) and Septin 9 Double Nickase Plasmid (h2) encode distinct paired gRNA designs targeting SEPT9. One or both designs may be available
- Following transfection, gene knockout efficiency can be assayed by WB, IF or IHC using antibody: Septin 9 Antibody (2C6): sc-293291
Ordering Information
| Product Name | Catalog # | UNIT | Price | Qty | FAVORITES | |
Septin 9 Double Nickase Plasmid (h) | sc-403987-NIC | 20 µg | $410.00 | |||
Septin 9 Double Nickase Plasmid (h2) | sc-403987-NIC-2 | 20 µg | $410.00 |
SEPT9 encodes septin 9, a GTP-binding cytoskeletal protein that assembles into hetero-oligomeric septin filaments and acts as a diffusion barrier and scaffold for actin and microtubule-associated processes. Septin 9 contributes to cytokinesis, vesicle trafficking, cell polarity, and membrane remodeling, and has been implicated in regulating mitotic progression and cellular architecture. Altered SEPT9 expression or genomic disruption is associated with defects in cell division and cytoskeletal organization, and SEPT9 dysregulation has been reported in diverse cancer contexts and other disorders involving aberrant proliferation and tissue organization. These properties make septin 9 a useful node for studying cytoskeletal dynamics, mitotic fidelity, and pathway-level consequences of septin network perturbation.
Septin 9 Double Nickase Plasmid (h) consists of a matched pair of plasmids engineered for high-specificity editing of the SEPT9 locus in human cell lines. Each plasmid expresses a Cas9 D10A nickase and a distinct sgRNA targeting opposite DNA strands within SEPT9. When directed to adjacent sites on opposite DNA strands, the two nickases generate offset single-strand nicks that together produce a staggered double-strand break, requiring coordinated on-target activity from both guides. The resulting DNA break is resolved by endogenous cellular repair pathways, most commonly through non-homologous end joining (NHEJ), leading to insertions or deletions that disrupt SEPT9 function. By requiring dual sgRNA engagement at the target locus, the double nicking approach enhances editing specificity and provides a complementary CRISPR strategy for applications where additional control over targeting precision is desired.
To support efficient identification of edited cells, one plasmid encodes GFP for fluorescent visualization of transfected populations, while the companion plasmid carries a puromycin resistance gene for antibiotic selection. Together, these features support efficient enrichment of co-transfected populations and simplify the validation of SEPT9-disrupted clones.
For Research Use Only. Not Intended for Diagnostic or Therapeutic Use.