Septin 6 Double Nickase Plasmid (h): sc-405243-NIC
- Target species: human
- 20 µg of transfection-ready, purified plasmid DNA; Suitable for up to 20 transfections
- Septin 6 Double Nickase Plasmid (h) consists of a pair of plasmids each encoding a D10A mutated Cas9 nuclease and a target-specific 20 nt guide RNA (gRNA) designed to knockout gene expression with greater specificity than its CRISPR/Cas9 KO counterpart
- Paired gRNA sequences are offset by approximately 20 bp to allow for specific Cas9-mediated double nicking of the genomic DNA, which mimics a DSB
- One plasmid in the pair contains a puromycin-resistance gene for selection; the other plasmid in the pair contains a GFP marker to visually confirm transfection
- Septin 6 Double Nickase Plasmid (h) and Septin 6 Double Nickase Plasmid (h2) encode distinct paired gRNA designs targeting SEPT6. One or both designs may be available
- Following transfection, gene knockout efficiency can be assayed by WB, IF or IHC using antibody: Septin 6 Antibody (B-8): sc-514781
Ordering Information
| Product Name | Catalog # | UNIT | Price | Qty | FAVORITES | |
Septin 6 Double Nickase Plasmid (h) | sc-405243-NIC | 20 µg | $410.00 | |||
Septin 6 Double Nickase Plasmid (h2) | sc-405243-NIC-2 | 20 µg | $410.00 |
SEPT6 encodes Septin 6, a conserved GTP-binding cytoskeletal protein that assembles into hetero-oligomeric septin filaments and rings to scaffold membranes and coordinate actin and microtubule dynamics. Septin 6 contributes to cytokinesis, cell polarity, vesicle trafficking, and compartmentalization at sites such as the cleavage furrow and neurite outgrowth regions, influencing cell shape and division fidelity. Through its roles in cytoskeletal remodeling and membrane organization, SEPT6 is studied in pathways controlling mitotic progression and intracellular transport. Dysregulated septin assembly or altered SEPT6 expression has been associated with abnormal proliferation and differentiation phenotypes, supporting its relevance in investigations of cancer biology and neurodevelopmental processes.
Septin 6 Double Nickase Plasmid (h) consists of a matched pair of plasmids engineered for high-specificity editing of the SEPT6 locus in human cell lines. Each plasmid expresses a Cas9 D10A nickase and a distinct sgRNA targeting opposite DNA strands within SEPT6. When directed to adjacent sites on opposite DNA strands, the two nickases generate offset single-strand nicks that together produce a staggered double-strand break, requiring coordinated on-target activity from both guides. The resulting DNA break is resolved by endogenous cellular repair pathways, most commonly through non-homologous end joining (NHEJ), leading to insertions or deletions that disrupt SEPT6 function. By requiring dual sgRNA engagement at the target locus, the double nicking approach enhances editing specificity and provides a complementary CRISPR strategy for applications where additional control over targeting precision is desired.
To support efficient identification of edited cells, one plasmid encodes GFP for fluorescent visualization of transfected populations, while the companion plasmid carries a puromycin resistance gene for antibiotic selection. Together, these features support efficient enrichment of co-transfected populations and simplify the validation of SEPT6-disrupted clones.
For Research Use Only. Not Intended for Diagnostic or Therapeutic Use.