Septin 3 CRISPR/Cas9 KO Plasmid (h): sc-406325

Overview

SEPT3 encodes Septin 3, a GTP-binding cytoskeletal protein in the septin family that assembles into hetero-oligomeric filaments and ring-like structures to organize membrane domains. Septin 3 contributes to actin- and microtubule-associated processes including vesicle trafficking, neurite outgrowth, and spatial control of cytokinesis and cell polarity through scaffold and diffusion-barrier functions. In the nervous system, SEPT3 expression is enriched in neurons and has been linked to regulation of synaptic architecture and vesicle dynamics, connecting it to pathways governing neuronal differentiation and synaptic transmission. Dysregulated septin network organization and SEPT3-associated alterations have been reported in studies of neurodegeneration and tumor biology, supporting its utility as a mechanistic node in cytoskeletal and membrane remodeling research.

Septin 3 CRISPR/Cas9 KO Plasmid (h) is a pool of plasmids designed for targeted disruption of the SEPT3 gene in human cell lines. Each plasmid co-expresses a unique single guide RNA (sgRNA) targeting a distinct site within the SEPT3 together with the Streptococcus pyogenes Cas9 nuclease. The plasmids also encode GFP, allowing fluorescent identification and enrichment of successfully transfected cells by fluorescence microscopy or flow cytometry.

The multi-guide design increases the likelihood of generating insertions or deletions (indels) that disrupt the SEPT3 open reading frame following Cas9-mediated double-strand break formation. DNA breaks introduced by the CRISPR/Cas9 system are repaired through endogenous non-homologous end joining (NHEJ) pathways, frequently resulting in frameshift mutations that abolish Septin 3 protein expression.

This CRISPR knockout system enables efficient generation of SEPT3-deficient cell models for investigation of Septin 3 signaling, functional genomics studies, cancer biology research, and evaluation of therapeutic responses in human cell lines.

Key Features

• sgRNAs targeting SEPT3 exon(s) critical for Septin 3 function
• Co-expression of SpCas9 and sgRNA from a single plasmid for simplified delivery
• GFP reporter for identification of transfected cells
• Pool of plasmids targeting multiple SEPT3 genomic sites to improve knockout efficiency
• Compatible with delivery by transfection

Design Variants

CRISPRs +/- HDRs

• gRNAs encoded by Septin 3 CRISPR/Cas9 KO Plasmid (h) and Septin 3 CRISPR/Cas9 KO Plasmid (h2) target distinct sites within the SEPT3 locus. One or both targeting designs may be available. See Related Products for availability.
• HDR donor constructs encoded by Septin 3 HDR Plasmid (h) and Septin 3 HDR Plasmid (h2) contain a puromycin resistance cassette and an RFP reporter flanked by SEPT3 homology arms to support homology-directed repair at defined SEPT3 target sites corresponding to the CRISPR/Cas9 KO designs. HDR donor availability may vary. See Related Products for availability.

For Research Use Only. Not Intended for Diagnostic or Therapeutic Use.