Septin 2 CRISPR/Cas9 KO Plasmid (m): sc-421849
- Target species: mouse
- 20 µg of transfection-ready, purified plasmid DNA; Suitable for up to 20 transfections
- Septin 2 CRISPR/Cas9 Knockout (KO) Plasmid (m) is a pool of plasmids, each encoding Cas9 nuclease and a target-specific 20 nt guide RNA (gRNA) designed for maximum knockout efficiency using sequences derived from the GeCKO v2 library
- gRNA sequences direct Cas9 to induce site-specific double-strand breaks (DSBs) in the Septin 2 genomic locus, resulting in gene knockout through non-homologous end joining (NHEJ)
- The puromycin resistance and RFP genes are flanked by LoxP sites, enabling removal of selection markers via Cre recombinase (Cre Vector: sc-418923) after establishing stable knockout cell lines
- Following transfection, gene knockout efficiency can be assayed by WB, IF or IHC using antibody: Septin 2 Antibody (F-1): sc-514206
Ordering Information
| Product Name | Catalog # | UNIT | Price | Qty | FAVORITES | |
Septin 2 CRISPR/Cas9 KO Plasmid (m) | sc-421849 | 20 µg | $397.00 |
Overview
Mouse Sept2 encodes septin 2, a core GTP-binding cytoskeletal protein that assembles into hetero-oligomeric septin filaments and ring-like structures to organize cell architecture. Septin 2 coordinates cytokinesis, membrane remodeling, and vesicle trafficking, and it interfaces with actin and microtubule networks to support polarized growth and intracellular compartmentalization. Sept2-dependent scaffolding contributes to processes such as midbody formation, neurite outgrowth, and epithelial barrier organization, linking septin dynamics to pathways governing cell division and morphogenesis. Dysregulated septin organization has been associated with aberrant mitosis and altered cytoskeletal signaling, making Sept2 a useful node for studying mechanisms relevant to tumor biology and neurodevelopmental phenotypes in experimental models.
Septin 2 CRISPR/Cas9 KO Plasmid (m) is a pool of plasmids designed for targeted disruption of the Sept2 gene in mouse cell lines. Each plasmid co-expresses a unique single guide RNA (sgRNA) targeting a distinct site within the Sept2 together with the Streptococcus pyogenes Cas9 nuclease. The plasmids also encode GFP, allowing fluorescent identification and enrichment of successfully transfected cells by fluorescence microscopy or flow cytometry.
The multi-guide design increases the likelihood of generating insertions or deletions (indels) that disrupt the Sept2 open reading frame following Cas9-mediated double-strand break formation. DNA breaks introduced by the CRISPR/Cas9 system are repaired through endogenous non-homologous end joining (NHEJ) pathways, frequently resulting in frameshift mutations that abolish Septin 2 protein expression.
This CRISPR knockout system enables efficient generation of Sept2-deficient cell models for investigation of Septin 2 signaling, functional genomics studies, cancer biology research, and evaluation of therapeutic responses in human cell lines.
Key Features
- sgRNAs targeting Sept2 exon(s) critical for Septin 2 function
- Co-expression of SpCas9 and sgRNA from a single plasmid for simplified delivery
- GFP reporter for identification of transfected cells
- Pool of plasmids targeting multiple Sept2 genomic sites to improve knockout efficiency
- Compatible with delivery by transfection
Design Variants
CRISPRs +/- HDRs
- gRNAs encoded by Septin 2 CRISPR/Cas9 KO Plasmid (m) and Septin 2 CRISPR/Cas9 KO Plasmid (m2) target distinct sites within the Sept2 locus. One or both targeting designs may be available. See Related Products for availability.
- HDR donor constructs encoded by Septin 2 HDR Plasmid (m) and Septin 2 HDR Plasmid (m2) contain a puromycin resistance cassette and an RFP reporter flanked by Sept2 homology arms to support homology-directed repair at defined Sept2 target sites corresponding to the CRISPR/Cas9 KO designs. HDR donor availability may vary. See Related Products for availability.
For Research Use Only. Not Intended for Diagnostic or Therapeutic Use.