Septin 14 CRISPR/Cas9 KO Plasmid (h): sc-406949
- Target species: human
- 20 µg of transfection-ready, purified plasmid DNA; Suitable for up to 20 transfections
- Septin 14 CRISPR/Cas9 Knockout (KO) Plasmid (h) is a pool of plasmids, each encoding Cas9 nuclease and a target-specific 20 nt guide RNA (gRNA) designed for maximum knockout efficiency using sequences derived from the GeCKO v2 library
- gRNA sequences direct Cas9 to induce site-specific double-strand breaks (DSBs) in the Septin 14 genomic locus, resulting in gene knockout through non-homologous end joining (NHEJ)
- The puromycin resistance and RFP genes are flanked by LoxP sites, enabling removal of selection markers via Cre recombinase (Cre Vector: sc-418923) after establishing stable knockout cell lines
Ordering Information
| Product Name | Catalog # | UNIT | Price | Qty | FAVORITES | |
Septin 14 CRISPR/Cas9 KO Plasmid (h) | sc-406949 | 20 µg | $397.00 |
Overview
SEPT14 encodes septin 14, a member of the septin family of GTP-binding cytoskeletal proteins that assemble into hetero-oligomeric filaments and higher-order rings. Septin 14 contributes to organization of the actin–microtubule interface, membrane remodeling, and establishment of diffusion barriers that support cytokinesis, cell polarity, and vesicle trafficking. Septin-dependent scaffolding also coordinates signaling and protein compartmentalization at the cortex, linking cytoskeletal dynamics to cell-cycle progression. Dysregulated septin expression or filament organization has been associated with altered proliferation and cellular architecture in disease-relevant contexts, supporting investigation of SEPT14 in mechanisms of cytoskeletal malfunction.
Septin 14 CRISPR/Cas9 KO Plasmid (h) is a pool of plasmids designed for targeted disruption of the SEPT14 gene in human cell lines. Each plasmid co-expresses a unique single guide RNA (sgRNA) targeting a distinct site within the SEPT14 together with the Streptococcus pyogenes Cas9 nuclease. The plasmids also encode GFP, allowing fluorescent identification and enrichment of successfully transfected cells by fluorescence microscopy or flow cytometry.
The multi-guide design increases the likelihood of generating insertions or deletions (indels) that disrupt the SEPT14 open reading frame following Cas9-mediated double-strand break formation. DNA breaks introduced by the CRISPR/Cas9 system are repaired through endogenous non-homologous end joining (NHEJ) pathways, frequently resulting in frameshift mutations that abolish Septin 14 protein expression.
This CRISPR knockout system enables efficient generation of SEPT14-deficient cell models for investigation of Septin 14 signaling, functional genomics studies, cancer biology research, and evaluation of therapeutic responses in human cell lines.
Key Features
- sgRNAs targeting SEPT14 exon(s) critical for Septin 14 function
- Co-expression of SpCas9 and sgRNA from a single plasmid for simplified delivery
- GFP reporter for identification of transfected cells
- Pool of plasmids targeting multiple SEPT14 genomic sites to improve knockout efficiency
- Compatible with delivery by transfection
Design Variants
CRISPRs +/- HDRs
- gRNAs encoded by Septin 14 CRISPR/Cas9 KO Plasmid (h) and Septin 14 CRISPR/Cas9 KO Plasmid (h2) target distinct sites within the SEPT14 locus. One or both targeting designs may be available. See Related Products for availability.
- HDR donor constructs encoded by Septin 14 HDR Plasmid (h) and Septin 14 HDR Plasmid (h2) contain a puromycin resistance cassette and an RFP reporter flanked by SEPT14 homology arms to support homology-directed repair at defined SEPT14 target sites corresponding to the CRISPR/Cas9 KO designs. HDR donor availability may vary. See Related Products for availability.
For Research Use Only. Not Intended for Diagnostic or Therapeutic Use.