SEMA6C CRISPR/Cas9 KO Plasmid (m): sc-422889
- Target species: mouse
- 20 µg of transfection-ready, purified plasmid DNA; Suitable for up to 20 transfections
- SEMA6C CRISPR/Cas9 Knockout (KO) Plasmid (m) is a pool of plasmids, each encoding Cas9 nuclease and a target-specific 20 nt guide RNA (gRNA) designed for maximum knockout efficiency using sequences derived from the GeCKO v2 library
- gRNA sequences direct Cas9 to induce site-specific double-strand breaks (DSBs) in the SEMA6C genomic locus, resulting in gene knockout through non-homologous end joining (NHEJ)
- The puromycin resistance and RFP genes are flanked by LoxP sites, enabling removal of selection markers via Cre recombinase (Cre Vector: sc-418923) after establishing stable knockout cell lines
- Following transfection, gene knockout efficiency can be assayed by WB, IF or IHC using antibody: SEMA6C Antibody (Ex-14): sc-74277
Ordering Information
| Product Name | Catalog # | UNIT | Price | Qty | FAVORITES | |
SEMA6C CRISPR/Cas9 KO Plasmid (m) | sc-422889 | 20 µg | $397.00 |
Overview
Sema6c encodes the class 6 semaphorin SEMA6C, a transmembrane guidance cue that regulates contact-dependent signaling during tissue patterning. Through interactions with plexin receptors and associated Rho family GTPase signaling, SEMA6C influences cytoskeletal remodeling, cell polarity, migration, and neurite outgrowth. Semaphorin–plexin pathways are also implicated in axon tract formation and synaptic connectivity, and altered semaphorin signaling has been linked to neurodevelopmental and neurodegenerative phenotypes in model systems. In addition, SEMA6 family members can modulate vascular and immune cell behavior, supporting investigation of cell–cell communication in development and inflammation-related contexts.
SEMA6C CRISPR/Cas9 KO Plasmid (m) is a pool of plasmids designed for targeted disruption of the Sema6c gene in mouse cell lines. Each plasmid co-expresses a unique single guide RNA (sgRNA) targeting a distinct site within the Sema6c together with the Streptococcus pyogenes Cas9 nuclease. The plasmids also encode GFP, allowing fluorescent identification and enrichment of successfully transfected cells by fluorescence microscopy or flow cytometry.
The multi-guide design increases the likelihood of generating insertions or deletions (indels) that disrupt the Sema6c open reading frame following Cas9-mediated double-strand break formation. DNA breaks introduced by the CRISPR/Cas9 system are repaired through endogenous non-homologous end joining (NHEJ) pathways, frequently resulting in frameshift mutations that abolish SEMA6C protein expression.
This CRISPR knockout system enables efficient generation of Sema6c-deficient cell models for investigation of SEMA6C signaling, functional genomics studies, cancer biology research, and evaluation of therapeutic responses in human cell lines.
Key Features
- sgRNAs targeting Sema6c exon(s) critical for SEMA6C function
- Co-expression of SpCas9 and sgRNA from a single plasmid for simplified delivery
- GFP reporter for identification of transfected cells
- Pool of plasmids targeting multiple Sema6c genomic sites to improve knockout efficiency
- Compatible with delivery by transfection
Design Variants
CRISPRs +/- HDRs
- gRNAs encoded by SEMA6C CRISPR/Cas9 KO Plasmid (m) and SEMA6C CRISPR/Cas9 KO Plasmid (m2) target distinct sites within the Sema6c locus. One or both targeting designs may be available. See Related Products for availability.
- HDR donor constructs encoded by SEMA6C HDR Plasmid (m) and SEMA6C HDR Plasmid (m2) contain a puromycin resistance cassette and an RFP reporter flanked by Sema6c homology arms to support homology-directed repair at defined Sema6c target sites corresponding to the CRISPR/Cas9 KO designs. HDR donor availability may vary. See Related Products for availability.
For Research Use Only. Not Intended for Diagnostic or Therapeutic Use.