SEMA4F CRISPR/Cas9 KO Plasmid (m): sc-422884
- Target species: mouse
- 20 µg of transfection-ready, purified plasmid DNA; Suitable for up to 20 transfections
- SEMA4F CRISPR/Cas9 Knockout (KO) Plasmid (m) is a pool of plasmids, each encoding Cas9 nuclease and a target-specific 20 nt guide RNA (gRNA) designed for maximum knockout efficiency using sequences derived from the GeCKO v2 library
- gRNA sequences direct Cas9 to induce site-specific double-strand breaks (DSBs) in the SEMA4F genomic locus, resulting in gene knockout through non-homologous end joining (NHEJ)
- The puromycin resistance and RFP genes are flanked by LoxP sites, enabling removal of selection markers via Cre recombinase (Cre Vector: sc-418923) after establishing stable knockout cell lines
Ordering Information
| Product Name | Catalog # | UNIT | Price | Qty | FAVORITES | |
SEMA4F CRISPR/Cas9 KO Plasmid (m) | sc-422884 | 20 µg | $397.00 |
Overview
Sema4f encodes the mouse semaphorin SEMA4F, a transmembrane guidance cue that helps coordinate cell–cell communication and spatial organization of tissues. SEMA4F engages plexin/neuropilin signaling to modulate cytoskeletal dynamics, neurite outgrowth, axon pathfinding, and synapse-associated remodeling through pathways that converge on Rho family GTPases and downstream actin regulation. In addition to nervous system development and plasticity, semaphorin signaling contributes to immune cell positioning and migratory behavior in diverse microenvironments. Dysregulated semaphorin–plexin networks have been implicated in neurodevelopmental and neuroinflammatory phenotypes, making Sema4f a useful node for dissecting guidance and motility programs in mouse models.
SEMA4F CRISPR/Cas9 KO Plasmid (m) is a pool of plasmids designed for targeted disruption of the Sema4f gene in mouse cell lines. Each plasmid co-expresses a unique single guide RNA (sgRNA) targeting a distinct site within the Sema4f together with the Streptococcus pyogenes Cas9 nuclease. The plasmids also encode GFP, allowing fluorescent identification and enrichment of successfully transfected cells by fluorescence microscopy or flow cytometry.
The multi-guide design increases the likelihood of generating insertions or deletions (indels) that disrupt the Sema4f open reading frame following Cas9-mediated double-strand break formation. DNA breaks introduced by the CRISPR/Cas9 system are repaired through endogenous non-homologous end joining (NHEJ) pathways, frequently resulting in frameshift mutations that abolish SEMA4F protein expression.
This CRISPR knockout system enables efficient generation of Sema4f-deficient cell models for investigation of SEMA4F signaling, functional genomics studies, cancer biology research, and evaluation of therapeutic responses in human cell lines.
Key Features
- sgRNAs targeting Sema4f exon(s) critical for SEMA4F function
- Co-expression of SpCas9 and sgRNA from a single plasmid for simplified delivery
- GFP reporter for identification of transfected cells
- Pool of plasmids targeting multiple Sema4f genomic sites to improve knockout efficiency
- Compatible with delivery by transfection
Design Variants
CRISPRs +/- HDRs
- gRNAs encoded by SEMA4F CRISPR/Cas9 KO Plasmid (m) and SEMA4F CRISPR/Cas9 KO Plasmid (m2) target distinct sites within the Sema4f locus. One or both targeting designs may be available. See Related Products for availability.
- HDR donor constructs encoded by SEMA4F HDR Plasmid (m) and SEMA4F HDR Plasmid (m2) contain a puromycin resistance cassette and an RFP reporter flanked by Sema4f homology arms to support homology-directed repair at defined Sema4f target sites corresponding to the CRISPR/Cas9 KO designs. HDR donor availability may vary. See Related Products for availability.
For Research Use Only. Not Intended for Diagnostic or Therapeutic Use.