SELH CRISPR/Cas9 KO Plasmid (h): sc-415276
- Target species: human
- 20 µg of transfection-ready, purified plasmid DNA; Suitable for up to 20 transfections
- SELH CRISPR/Cas9 Knockout (KO) Plasmid (h) is a pool of plasmids, each encoding Cas9 nuclease and a target-specific 20 nt guide RNA (gRNA) designed for maximum knockout efficiency using sequences derived from the GeCKO v2 library
- gRNA sequences direct Cas9 to induce site-specific double-strand breaks (DSBs) in the SELH genomic locus, resulting in gene knockout through non-homologous end joining (NHEJ)
- The puromycin resistance and RFP genes are flanked by LoxP sites, enabling removal of selection markers via Cre recombinase (Cre Vector: sc-418923) after establishing stable knockout cell lines
Ordering Information
| Product Name | Catalog # | UNIT | Price | Qty | FAVORITES | |
SELH CRISPR/Cas9 KO Plasmid (h) | sc-415276 | 20 µg | $397.00 |
Overview
SELENOH encodes selenoprotein H (SELH), a small redox-active protein enriched in the nucleus that contributes to cellular antioxidant defense and maintenance of redox homeostasis through selenium-dependent catalytic activity. SELH is linked to regulation of reactive oxygen species buffering, genome stability, and stress-responsive transcriptional programs that influence cell survival and differentiation. As part of broader selenoprotein biology, SELH intersects with glutathione- and thioredoxin-associated redox networks and can modulate mitochondrial and endoplasmic reticulum stress responses. Altered SELENOH expression or selenium handling has been explored in contexts of neurodegeneration, metabolic dysfunction, and cancer-associated oxidative stress phenotypes, motivating mechanistic studies in human cell models.
SELH CRISPR/Cas9 KO Plasmid (h) is a pool of plasmids designed for targeted disruption of the SELENOH gene in human cell lines. Each plasmid co-expresses a unique single guide RNA (sgRNA) targeting a distinct site within the SELENOH together with the Streptococcus pyogenes Cas9 nuclease. The plasmids also encode GFP, allowing fluorescent identification and enrichment of successfully transfected cells by fluorescence microscopy or flow cytometry.
The multi-guide design increases the likelihood of generating insertions or deletions (indels) that disrupt the SELENOH open reading frame following Cas9-mediated double-strand break formation. DNA breaks introduced by the CRISPR/Cas9 system are repaired through endogenous non-homologous end joining (NHEJ) pathways, frequently resulting in frameshift mutations that abolish SELH protein expression.
This CRISPR knockout system enables efficient generation of SELENOH-deficient cell models for investigation of SELH signaling, functional genomics studies, cancer biology research, and evaluation of therapeutic responses in human cell lines.
Key Features
- sgRNAs targeting SELENOH exon(s) critical for SELH function
- Co-expression of SpCas9 and sgRNA from a single plasmid for simplified delivery
- GFP reporter for identification of transfected cells
- Pool of plasmids targeting multiple SELENOH genomic sites to improve knockout efficiency
- Compatible with delivery by transfection
Design Variants
CRISPRs +/- HDRs
- gRNAs encoded by SELH CRISPR/Cas9 KO Plasmid (h) and SELH CRISPR/Cas9 KO Plasmid (h2) target distinct sites within the SELENOH locus. One or both targeting designs may be available. See Related Products for availability.
- HDR donor constructs encoded by SELH HDR Plasmid (h) and SELH HDR Plasmid (h2) contain a puromycin resistance cassette and an RFP reporter flanked by SELENOH homology arms to support homology-directed repair at defined SELENOH target sites corresponding to the CRISPR/Cas9 KO designs. HDR donor availability may vary. See Related Products for availability.
For Research Use Only. Not Intended for Diagnostic or Therapeutic Use.