Not CRISPR data: the image illustrates antibody quality for visualizing CRISPR experiment results.
Not CRISPR data: the image illustrates antibody quality for visualizing CRISPR experiment results.
SELENBP1 CRISPR/Cas9 KO Plasmid (m): sc-422870
- Target species: mouse
- 20 µg of transfection-ready, purified plasmid DNA; Suitable for up to 20 transfections
- SELENBP1 CRISPR/Cas9 Knockout (KO) Plasmid (m) is a pool of plasmids, each encoding Cas9 nuclease and a target-specific 20 nt guide RNA (gRNA) designed for maximum knockout efficiency using sequences derived from the GeCKO v2 library
- gRNA sequences direct Cas9 to induce site-specific double-strand breaks (DSBs) in the SELENBP1 genomic locus, resulting in gene knockout through non-homologous end joining (NHEJ)
- The puromycin resistance and RFP genes are flanked by LoxP sites, enabling removal of selection markers via Cre recombinase (Cre Vector: sc-418923) after establishing stable knockout cell lines
Ordering Information
| Product Name | Catalog # | UNIT | Price | Qty | FAVORITES | |
SELENBP1 CRISPR/Cas9 KO Plasmid (m) | sc-422870 | 20 µg | $397.00 |
Overview
Selenbp1 encodes selenium-binding protein 1 (SELENBP1), a cytosolic protein implicated in cellular selenium handling and redox-associated metabolism. In mouse tissues, SELENBP1 is linked to regulation of oxidative stress responses and metabolic adaptation, with reported connections to xenobiotic processing and cellular differentiation programs. Altered SELENBP1 abundance has been associated with changes in proliferation and stress signaling in multiple experimental systems, making it relevant for studying context-dependent metabolic and redox phenotypes. As a selenium-associated factor, SELENBP1 provides a tractable node for dissecting how selenium utilization interfaces with homeostatic pathways that shape cellular fitness.
SELENBP1 CRISPR/Cas9 KO Plasmid (m) is a pool of plasmids designed for targeted disruption of the Selenbp1 gene in mouse cell lines. Each plasmid co-expresses a unique single guide RNA (sgRNA) targeting a distinct site within the Selenbp1 together with the Streptococcus pyogenes Cas9 nuclease. The plasmids also encode GFP, allowing fluorescent identification and enrichment of successfully transfected cells by fluorescence microscopy or flow cytometry.
The multi-guide design increases the likelihood of generating insertions or deletions (indels) that disrupt the Selenbp1 open reading frame following Cas9-mediated double-strand break formation. DNA breaks introduced by the CRISPR/Cas9 system are repaired through endogenous non-homologous end joining (NHEJ) pathways, frequently resulting in frameshift mutations that abolish SELENBP1 protein expression.
This CRISPR knockout system enables efficient generation of Selenbp1-deficient cell models for investigation of SELENBP1 signaling, functional genomics studies, cancer biology research, and evaluation of therapeutic responses in human cell lines.
Key Features
- sgRNAs targeting Selenbp1 exon(s) critical for SELENBP1 function
- Co-expression of SpCas9 and sgRNA from a single plasmid for simplified delivery
- GFP reporter for identification of transfected cells
- Pool of plasmids targeting multiple Selenbp1 genomic sites to improve knockout efficiency
- Compatible with delivery by transfection
Design Variants
CRISPRs +/- HDRs
- gRNAs encoded by SELENBP1 CRISPR/Cas9 KO Plasmid (m) and SELENBP1 CRISPR/Cas9 KO Plasmid (m2) target distinct sites within the Selenbp1 locus. One or both targeting designs may be available. See Related Products for availability.
- HDR donor constructs encoded by SELENBP1 HDR Plasmid (m) and SELENBP1 HDR Plasmid (m2) contain a puromycin resistance cassette and an RFP reporter flanked by Selenbp1 homology arms to support homology-directed repair at defined Selenbp1 target sites corresponding to the CRISPR/Cas9 KO designs. HDR donor availability may vary. See Related Products for availability.
For Research Use Only. Not Intended for Diagnostic or Therapeutic Use.