SELB Antibody (G-9): sc-166521

SELB Antibody (G-9) is a mouse monoclonal IgG1 kappa light chain antibody that detects SELB protein of mouse, rat, and human origin by western blotting (WB), immunoprecipitation (IP), immunofluorescence (IF), immunohistochemistry, and enzyme-linked immunosorbent assay (ELISA). SELB (G-9) antibody is available in both non-conjugated and various conjugated forms, including agarose, horseradish peroxidase (HRP), phycoerythrin (PE), fluorescein isothiocyanate (FITC), and multiple Alexa Fluor® conjugates. SELB protein, also known as selenocysteine-specific elongation factor or EEFSEC, is a crucial 596 amino acid protein that plays a vital role in the translation process by facilitating selenocysteine incorporation into proteins, which is essential for proper functioning of various enzymes and proteins that rely on this unique amino acid. SELB localization in both nucleus and cytoplasm underscores SELB′s importance in cellular processes, as SELB is involved in synthesis of proteins critical for cellular metabolism and signaling pathways. SELB gene is located on human chromosome 3, a region harboring over 1,100 genes, including several implicated in cancer and other genetic disorders. Notably, chromosome 3 contains key tumor suppressor genes, such as RASSF1, which is involved in apoptosis, and HYAL1, which regulates cell migration, highlighting SELB′s potential implications in cancer biology and therapeutic strategies.

Alexa Fluor^® is a trademark of Molecular Probes Inc., OR., USA

LI-COR^® and Odyssey^® are registered trademarks of LI-COR Biosciences

SELB Antibody (G-9) References:

1. Characterization of mSelB, a novel mammalian elongation factor for selenoprotein translation.  |  Fagegaltier, D., et al. 2000. EMBO J. 19: 4796-805. PMID: 10970870
2. Coupled tRNA(Sec)-dependent assembly of the selenocysteine decoding apparatus.  |  Zavacki, AM., et al. 2003. Mol Cell. 11: 773-81. PMID: 12667458
3. Efficiency of mammalian selenocysteine incorporation.  |  Mehta, A., et al. 2004. J Biol Chem. 279: 37852-9. PMID: 15229221
4. Size matters: a view of selenocysteine incorporation from the ribosome.  |  Caban, K. and Copeland, PR. 2006. Cell Mol Life Sci. 63: 73-81. PMID: 16416259
5. The L7Ae RNA binding motif is a multifunctional domain required for the ribosome-dependent Sec incorporation activity of Sec insertion sequence binding protein 2.  |  Caban, K., et al. 2007. Mol Cell Biol. 27: 6350-60. PMID: 17636016
6. Functional analysis of the interplay between translation termination, selenocysteine codon context, and selenocysteine insertion sequence-binding protein 2.  |  Gupta, M. and Copeland, PR. 2007. J Biol Chem. 282: 36797-807. PMID: 17954931
7. A novel protein domain induces high affinity selenocysteine insertion sequence binding and elongation factor recruitment.  |  Donovan, J., et al. 2008. J Biol Chem. 283: 35129-39. PMID: 18948268

Monoclonal antibodies for mammalian and non-mammalian protein targets represent essentially all targets covered by polyclonal antibodies. Over 8,300 of our antibodies are offered as ImmunoCruz^® conjugates which eliminates the need for a secondary antibody.

Santa Cruz Biotechnology antibodies have over 360,000 research citations. Anti-SELB Antibody (G-9) has 0 citations in a variety of scientific publications.

Primary antibodies like Anti-SELB Antibody (G-9) for mammalian target proteins are recommended for the detection of a range of mammalian species, primarily of mouse, rat and human species. Many mammalian antibodies are also ideal for veterinary research and reactive with ovine, porcine, caprine, feline, canine, bovine and equine protein targets. Santa Cruz Biotechnology primary monoclonal antibodies are suitable for laboratory research applications and are not intended for diagnostic or therapeutic use.

SELB Antibody (G-9) Specifications

Alternate Product Name: SELB

Product Clone Name: G-9

Classification: Primary Antibody

Clonality: Monoclonal Antibody

Antibody Isotype: IgG₁ kappa light chain

Amino Acids: 211-450

Epitope Target: SELB

Epitope Species: human

Product Reactivity/Detection: SELB

Species Reactivity/Detection: mouse, rat, human

Additional Mammalian Reactivity: N/A

Additional Non-Mammalian Reactivity: N/A

Applications: WB, IP, IF, IHC(P), ELISA

Vialing Information: Each vial contains 200 µg IgG₁ kappa light chain in 1.0 ml of PBS with < 0.1% sodium azide and 0.1% gelatin

Research Citations: 0 citations in various scientific publications

Available Conjugates/Forms for SELB Antibody (G-9): AC: Agarose, AF488: Alexa Fluor^® 488, AF546: Alexa Fluor^® 546, AF594: Alexa Fluor^® 594, AF647: Alexa Fluor^® 647, AF680: Alexa Fluor^® 680, AF790: Alexa Fluor^® 790, FITC: Fluoroscein, HRP: Horseradish Peroxidase, PE: Phycoerythrin