Sec61α1 CRISPR/Cas9 KO Plasmid (m): sc-424716
- Target species: mouse
- 20 µg of transfection-ready, purified plasmid DNA; Suitable for up to 20 transfections
- Sec61α1 CRISPR/Cas9 Knockout (KO) Plasmid (m) is a pool of plasmids, each encoding Cas9 nuclease and a target-specific 20 nt guide RNA (gRNA) designed for maximum knockout efficiency using sequences derived from the GeCKO v2 library
- gRNA sequences direct Cas9 to induce site-specific double-strand breaks (DSBs) in the Sec61α1 genomic locus, resulting in gene knockout through non-homologous end joining (NHEJ)
- The puromycin resistance and RFP genes are flanked by LoxP sites, enabling removal of selection markers via Cre recombinase (Cre Vector: sc-418923) after establishing stable knockout cell lines
Ordering Information
| Product Name | Catalog # | UNIT | Price | Qty | FAVORITES | |
Sec61α1 CRISPR/Cas9 KO Plasmid (m) | sc-424716 | 20 µg | $397.00 |
Overview
Sec61a1 encodes Sec61α1, the pore-forming core subunit of the Sec61 translocon in the endoplasmic reticulum (ER) membrane that mediates co-translational and post-translational import of secretory and membrane proteins. By coupling ribosome docking with peptide translocation and integration, Sec61α1 supports protein biogenesis, ER quality control, and proteostasis pathways including unfolded protein response signaling and ER-associated degradation. Perturbation of SEC61 channel function has been linked to defects in secretory pathway homeostasis and cellular stress responses, processes frequently implicated in inflammation, metabolic dysfunction, and neurodegeneration models. In mouse systems, Sec61a1 provides a tractable entry point to study how ER import capacity shapes signaling, differentiation, and survival under proteotoxic stress.
Sec61α1 CRISPR/Cas9 KO Plasmid (m) is a pool of plasmids designed for targeted disruption of the Sec61a1 gene in mouse cell lines. Each plasmid co-expresses a unique single guide RNA (sgRNA) targeting a distinct site within the Sec61a1 together with the Streptococcus pyogenes Cas9 nuclease. The plasmids also encode GFP, allowing fluorescent identification and enrichment of successfully transfected cells by fluorescence microscopy or flow cytometry.
The multi-guide design increases the likelihood of generating insertions or deletions (indels) that disrupt the Sec61a1 open reading frame following Cas9-mediated double-strand break formation. DNA breaks introduced by the CRISPR/Cas9 system are repaired through endogenous non-homologous end joining (NHEJ) pathways, frequently resulting in frameshift mutations that abolish Sec61α1 protein expression.
This CRISPR knockout system enables efficient generation of Sec61a1-deficient cell models for investigation of Sec61α1 signaling, functional genomics studies, cancer biology research, and evaluation of therapeutic responses in human cell lines.
Key Features
- sgRNAs targeting Sec61a1 exon(s) critical for Sec61α1 function
- Co-expression of SpCas9 and sgRNA from a single plasmid for simplified delivery
- GFP reporter for identification of transfected cells
- Pool of plasmids targeting multiple Sec61a1 genomic sites to improve knockout efficiency
- Compatible with delivery by transfection
Design Variants
CRISPRs +/- HDRs
- gRNAs encoded by Sec61α1 CRISPR/Cas9 KO Plasmid (m) and Sec61α1 CRISPR/Cas9 KO Plasmid (m2) target distinct sites within the Sec61a1 locus. One or both targeting designs may be available. See Related Products for availability.
- HDR donor constructs encoded by Sec61α1 HDR Plasmid (m) and Sec61α1 HDR Plasmid (m2) contain a puromycin resistance cassette and an RFP reporter flanked by Sec61a1 homology arms to support homology-directed repair at defined Sec61a1 target sites corresponding to the CRISPR/Cas9 KO designs. HDR donor availability may vary. See Related Products for availability.
For Research Use Only. Not Intended for Diagnostic or Therapeutic Use.