SEC24A CRISPR/Cas9 KO Plasmid (m): sc-429522
- Target species: mouse
- 20 µg of transfection-ready, purified plasmid DNA; Suitable for up to 20 transfections
- SEC24A CRISPR/Cas9 Knockout (KO) Plasmid (m) is a pool of plasmids, each encoding Cas9 nuclease and a target-specific 20 nt guide RNA (gRNA) designed for maximum knockout efficiency using sequences derived from the GeCKO v2 library
- gRNA sequences direct Cas9 to induce site-specific double-strand breaks (DSBs) in the SEC24A genomic locus, resulting in gene knockout through non-homologous end joining (NHEJ)
- The puromycin resistance and RFP genes are flanked by LoxP sites, enabling removal of selection markers via Cre recombinase (Cre Vector: sc-418923) after establishing stable knockout cell lines
Ordering Information
| Product Name | Catalog # | UNIT | Price | Qty | FAVORITES | |
SEC24A CRISPR/Cas9 KO Plasmid (m) | sc-429522 | 20 µg | $397.00 |
Overview
Sec24a encodes SEC24A, a core component of the COPII coat that selects cargo and drives anterograde transport from the endoplasmic reticulum to the Golgi apparatus. By partnering with SEC23 and interacting with cargo adaptors, SEC24A helps regulate secretory trafficking, ER proteostasis, and membrane homeostasis that influence lipid handling and cell-surface receptor composition. Disruption of COPII-dependent export can trigger ER stress responses and alter extracellular matrix and signaling molecule delivery, linking SEC24A function to pathways governing metabolism and tissue homeostasis. In mice, Sec24a has been used to interrogate how selective cargo packaging impacts systemic lipid and lipoprotein biology and broader secretory pathway phenotypes.
SEC24A CRISPR/Cas9 KO Plasmid (m) is a pool of plasmids designed for targeted disruption of the Sec24a gene in mouse cell lines. Each plasmid co-expresses a unique single guide RNA (sgRNA) targeting a distinct site within the Sec24a together with the Streptococcus pyogenes Cas9 nuclease. The plasmids also encode GFP, allowing fluorescent identification and enrichment of successfully transfected cells by fluorescence microscopy or flow cytometry.
The multi-guide design increases the likelihood of generating insertions or deletions (indels) that disrupt the Sec24a open reading frame following Cas9-mediated double-strand break formation. DNA breaks introduced by the CRISPR/Cas9 system are repaired through endogenous non-homologous end joining (NHEJ) pathways, frequently resulting in frameshift mutations that abolish SEC24A protein expression.
This CRISPR knockout system enables efficient generation of Sec24a-deficient cell models for investigation of SEC24A signaling, functional genomics studies, cancer biology research, and evaluation of therapeutic responses in human cell lines.
Key Features
- sgRNAs targeting Sec24a exon(s) critical for SEC24A function
- Co-expression of SpCas9 and sgRNA from a single plasmid for simplified delivery
- GFP reporter for identification of transfected cells
- Pool of plasmids targeting multiple Sec24a genomic sites to improve knockout efficiency
- Compatible with delivery by transfection
Design Variants
CRISPRs +/- HDRs
- gRNAs encoded by SEC24A CRISPR/Cas9 KO Plasmid (m) and SEC24A CRISPR/Cas9 KO Plasmid (m2) target distinct sites within the Sec24a locus. One or both targeting designs may be available. See Related Products for availability.
- HDR donor constructs encoded by SEC24A HDR Plasmid (m) and SEC24A HDR Plasmid (m2) contain a puromycin resistance cassette and an RFP reporter flanked by Sec24a homology arms to support homology-directed repair at defined Sec24a target sites corresponding to the CRISPR/Cas9 KO designs. HDR donor availability may vary. See Related Products for availability.
For Research Use Only. Not Intended for Diagnostic or Therapeutic Use.