SEC14L2/L3/L4 Antibody (E-10): sc-365420

SEC14L2/L3/L4 Antibody (E-10) is a mouse monoclonal IgG1 kappa light chain antibody that detects SEC14L2/L3/L4 protein of mouse, rat, and human origin by western blotting (WB), immunoprecipitation (IP), immunofluorescence (IF), immunohistochemistry, and enzyme-linked immunosorbent assay (ELISA). SEC14L2/L3/L4 (E-10) antibody is available in both non-conjugated and various conjugated forms, including agarose, horseradish peroxidase (HRP), phycoerythrin (PE), fluorescein isothiocyanate (FITC), and multiple Alexa Fluor® conjugates. SEC14L2 protein, also known as supernatant protein factor, α-tocopherol-associated protein, or squalene transfer protein, plays a crucial role as a carrier protein that facilitates tocopherol transfer and acts as a transcriptional activator through α-tocopherol interaction. SEC14L2 participates in converting microsomal squalene-2,3-oxide into lanosterol, a key step in cholesterol biosynthesis. High SEC14L2 expression levels are found in liver, brain, intestine, and prostate, highlighting SEC14L2′s importance in various physiological processes. While SEC14L2 primarily localizes in cytoplasm, α-tocopherol presence prompts SEC14L2 translocation to nucleus, indicating a potential regulatory role in gene expression. SEC14L2 activity is significantly influenced by post-translational modifications, particularly phosphorylation by protein kinase A and protein kinase C, which can modulate SEC14L2 function and interactions. SEC14L3 and SEC14L4, which share structural domains with SEC14L2, may also participate in hydrophobic ligand transport, further underscoring functional diversity within SEC14 family proteins.

Alexa Fluor^® is a trademark of Molecular Probes Inc., OR., USA

LI-COR^® and Odyssey^® are registered trademarks of LI-COR Biosciences

SEC14L2/L3/L4 Antibody (E-10) References:

1. Supernatant protein factor, which stimulates the conversion of squalene to lanosterol, is a cytosolic squalene transfer protein and enhances cholesterol biosynthesis.  |  Shibata, N., et al. 2001. Proc Natl Acad Sci U S A. 98: 2244-9. PMID: 11226224
2. Phosphorylation of supernatant protein factor enhances its ability to stimulate microsomal squalene monooxygenase.  |  Singh, DK., et al. 2003. J Biol Chem. 278: 5646-51. PMID: 12454003
3. Supernatant protein factor in complex with RRR-alpha-tocopherylquinone: a link between oxidized Vitamin E and cholesterol biosynthesis.  |  Stocker, A. and Baumann, U. 2003. J Mol Biol. 332: 759-65. PMID: 12972248
4. Supernatant protein factor stimulates HMG-CoA reductase in cell culture and in vitro.  |  Mokashi, V., et al. 2005. Arch Biochem Biophys. 433: 474-80. PMID: 15581604
5. Regulation of hepatic cholesterol synthesis by a novel protein (SPF) that accelerates cholesterol biosynthesis.  |  Shibata, N., et al. 2006. FASEB J. 20: 2642-4. PMID: 17077281
6. Effects of a supernatant protein activator on microsomal squalene-2,3-oxide-lanosterol cyclase.  |  Caras, IW. and Bloch, K. 1979. J Biol Chem. 254: 11816-21. PMID: 500676
7. Role of supernatant protein factor and anionic phospholipid in squalene uptake and conversion by microsomes.  |  Chin, J. and Bloch, K. 1984. J Biol Chem. 259: 11735-8. PMID: 6480582
8. Supernatant protein factor facilitates intermembrane transfer of squalene.  |  Friedlander, EJ., et al. 1980. J Biol Chem. 255: 8042-5. PMID: 7410347

Monoclonal antibodies for mammalian and non-mammalian protein targets represent essentially all targets covered by polyclonal antibodies. Over 8,300 of our antibodies are offered as ImmunoCruz^® conjugates which eliminates the need for a secondary antibody.

Santa Cruz Biotechnology antibodies have over 360,000 research citations. Anti-SEC14L2/L3/L4 Antibody (E-10) has 0 citations in a variety of scientific publications.

Primary antibodies like Anti-SEC14L2/L3/L4 Antibody (E-10) for mammalian target proteins are recommended for the detection of a range of mammalian species, primarily of mouse, rat and human species. Many mammalian antibodies are also ideal for veterinary research and reactive with ovine, porcine, caprine, feline, canine, bovine and equine protein targets. Santa Cruz Biotechnology primary monoclonal antibodies are suitable for laboratory research applications and are not intended for diagnostic or therapeutic use.

SEC14L2/L3/L4 Antibody (E-10) Specifications

Alternate Product Name: SEC14L2/L3/L4

Product Clone Name: E-10

Classification: Primary Antibody

Clonality: Monoclonal Antibody

Antibody Isotype: IgG₁ kappa light chain

Amino Acids: 26-55

Epitope Target: SEC14L2

Epitope Species: human

Product Reactivity/Detection: SEC14L2, SEC14L3 and SEC14L4

Species Reactivity/Detection: mouse, rat, human

Additional Mammalian Reactivity: N/A

Additional Non-Mammalian Reactivity: N/A

Applications: WB, IP, IF, IHC(P), ELISA

Vialing Information: Each vial contains 200 µg IgG₁ kappa light chain in 1.0 ml of PBS with < 0.1% sodium azide and 0.1% gelatin

Research Citations: 0 citations in various scientific publications

Available Conjugates/Forms for SEC14L2/L3/L4 Antibody (E-10): AC: Agarose, AF488: Alexa Fluor^® 488, AF546: Alexa Fluor^® 546, AF594: Alexa Fluor^® 594, AF647: Alexa Fluor^® 647, AF680: Alexa Fluor^® 680, AF790: Alexa Fluor^® 790, FITC: Fluoroscein, HRP: Horseradish Peroxidase, P: Blocking Peptide, PE: Phycoerythrin