SDHC Double Nickase Plasmid (h): sc-405559-NIC
- Target species: human
- 20 µg of transfection-ready, purified plasmid DNA; Suitable for up to 20 transfections
- SDHC Double Nickase Plasmid (h) consists of a pair of plasmids each encoding a D10A mutated Cas9 nuclease and a target-specific 20 nt guide RNA (gRNA) designed to knockout gene expression with greater specificity than its CRISPR/Cas9 KO counterpart
- Paired gRNA sequences are offset by approximately 20 bp to allow for specific Cas9-mediated double nicking of the genomic DNA, which mimics a DSB
- One plasmid in the pair contains a puromycin-resistance gene for selection; the other plasmid in the pair contains a GFP marker to visually confirm transfection
- SDHC Double Nickase Plasmid (h) and SDHC Double Nickase Plasmid (h2) encode distinct paired gRNA designs targeting SDHC. One or both designs may be available
- Following transfection, gene knockout efficiency can be assayed by WB, IF or IHC using antibody: SDHC Antibody (C-2): sc-515102
Ordering Information
| Product Name | Catalog # | UNIT | Price | Qty | FAVORITES | |
SDHC Double Nickase Plasmid (h) | sc-405559-NIC | 20 µg | $410.00 |
SDHC encodes the membrane-anchored succinate dehydrogenase complex subunit C, an essential component of mitochondrial complex II that links the tricarboxylic acid (TCA) cycle to the electron transport chain by supporting succinate oxidation and electron transfer to ubiquinone. As part of the inner mitochondrial membrane anchor, SDHC helps stabilize complex II assembly and enables efficient oxidative phosphorylation, influencing cellular redox balance and reactive oxygen species handling. Disruption of SDHC can impair mitochondrial respiration and promote succinate accumulation, with downstream effects on metabolic signaling and epigenetic regulation through altered α-ketoglutarate–dependent dioxygenase activity. SDHC alterations are associated with mitochondrial dysfunction and have been implicated in hereditary paraganglioma/pheochromocytoma biology, making it a relevant target for studying metabolic reprogramming and stress-response pathways.
SDHC Double Nickase Plasmid (h) consists of a matched pair of plasmids engineered for high-specificity editing of the SDHC locus in human cell lines. Each plasmid expresses a Cas9 D10A nickase and a distinct sgRNA targeting opposite DNA strands within SDHC. When directed to adjacent sites on opposite DNA strands, the two nickases generate offset single-strand nicks that together produce a staggered double-strand break, requiring coordinated on-target activity from both guides. The resulting DNA break is resolved by endogenous cellular repair pathways, most commonly through non-homologous end joining (NHEJ), leading to insertions or deletions that disrupt SDHC function. By requiring dual sgRNA engagement at the target locus, the double nicking approach enhances editing specificity and provides a complementary CRISPR strategy for applications where additional control over targeting precision is desired.
To support efficient identification of edited cells, one plasmid encodes GFP for fluorescent visualization of transfected populations, while the companion plasmid carries a puromycin resistance gene for antibiotic selection. Together, these features support efficient enrichment of co-transfected populations and simplify the validation of SDHC-disrupted clones.
For Research Use Only. Not Intended for Diagnostic or Therapeutic Use.