SDHA Antibody (F-2): sc-390381

SDHA Antibody (F-2) is a mouse monoclonal IgG2a kappa light chain antibody that detects SDHA protein of mouse, rat, and human origin by western blotting (WB), immunoprecipitation (IP), immunofluorescence (IF), immunohistochemistry, and enzyme-linked immunosorbent assay (ELISA). Anti-SDHA antibody (F-2) is available in both non-conjugated and various conjugated forms, including agarose, horseradish peroxidase (HRP), phycoerythrin (PE), fluorescein isothiocyanate (FITC), and multiple Alexa Fluor® conjugates. SDHA protein, a crucial component of the succinate dehydrogenase complex, plays a vital role in the tricarboxylic acid cycle and electron transport chain, facilitating the conversion of succinate to fumarate while simultaneously reducing ubiquinone to ubiquinol. This dual function is essential for cellular respiration and energy production, highlighting SDHA′s importance in maintaining mitochondrial function. Disruptions in SDHA can lead to severe mitochondrial dysfunction, which is linked to various genetic disorders, including juvenile encephalopathy and Leigh Syndrome, underscoring SDHA′s critical nature in human health. Additionally, mutations in SDHA have been associated with hereditary paraganglioma, while inactivating mutations in SDHB correlate with inherited cases of pheochromocytoma. SDHA (F-2) antibody′s ability to effectively detect across multiple applications makes this product an invaluable tool for researchers studying mitochondrial function and related pathologies.

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SDHA Antibody (F-2) References:

1. Histochemical investigations on the presence of acetylcholinesterase and succinic dehydrogenase in fetal human spinal cord and brain stem at different stages of development.  |  Wolf, P., et al. 1975. Eur Neurol. 13: 31-46. PMID: 1126345
2. Classification of muscle fiber types based on succinic dehydrogenase and myofibrillar ATPase reactions in normal and randomly reinnervated rat skeletal muscles.  |  Rymaszewska-Kossakowska, T. and Sliwowski, A. 1977. Folia Histochem Cytochem (Krakow). 15: 43-8. PMID: 140839
3. Time course of changes in human skeletal muscle succinate dehydrogenase and cytochrome oxidase activities and maximal oxygen uptake with physical activity and inactivity.  |  Henriksson, J. and Reitman, JS. 1977. Acta Physiol Scand. 99: 91-7. PMID: 190867
4. SDHA mutations in adult and pediatric wild-type gastrointestinal stromal tumors.  |  Oudijk, L., et al. 2013. Mod Pathol. 26: 456-63. PMID: 23174939
5. Immunohistochemical loss of succinate dehydrogenase subunit A (SDHA) in gastrointestinal stromal tumors (GISTs) signals SDHA germline mutation.  |  Miettinen, M., et al. 2013. Am J Surg Pathol. 37: 234-40. PMID: 23282968
6. SDHA mutations causing a multisystem mitochondrial disease: novel mutations and genetic overlap with hereditary tumors.  |  Renkema, GH., et al. 2015. Eur J Hum Genet. 23: 202-9. PMID: 24781757
7. Proteins of the inner membrane of Escherichia coli: identification of succinate dehydrogenase by polyacrylamide gel electrophoresis with sdh amber mutants.  |  Spencer, ME. and Guest, JR. 1974. J Bacteriol. 117: 947-53. PMID: 4591960
8. Mutation of a nuclear succinate dehydrogenase gene results in mitochondrial respiratory chain deficiency.  |  Bourgeron, T., et al. 1995. Nat Genet. 11: 144-9. PMID: 7550341
9. Human complex II (succinate-ubiquinone oxidoreductase): cDNA cloning of the flavoprotein (Fp) subunit of liver mitochondria.  |  Hirawake, H., et al. 1994. J Biochem. 116: 221-7. PMID: 7798181
10. The effects of different patterns of muscle activity on capillary density, mechanical properties and structure of slow and fast rabbit muscles.  |  Brown, MD., et al. 1976. Pflugers Arch. 361: 241-50. PMID: 943767
11. Effect of low dose x-irradiation on the succinate dehydrogenase activity of guinea pig, rat and mouse tissues.  |  Shah, VC., et al. 1976. Strahlentherapie. 152: 83-91. PMID: 951722
12. Gene mutations in the succinate dehydrogenase subunit SDHB cause susceptibility to familial pheochromocytoma and to familial paraganglioma.  |  Astuti, D., et al. 2001. Am J Hum Genet. 69: 49-54. PMID: 11404820

Monoclonal antibodies for mammalian and non-mammalian protein targets represent essentially all targets covered by polyclonal antibodies. Over 8,300 of our antibodies are offered as ImmunoCruz^® conjugates which eliminates the need for a secondary antibody.

Santa Cruz Biotechnology antibodies have over 360,000 research citations. Anti-SDHA Antibody (F-2) has 17 citations in a variety of scientific publications.

Primary antibodies like Anti-SDHA Antibody (F-2) for mammalian target proteins are recommended for the detection of a range of mammalian species, primarily of mouse, rat and human species. Many mammalian antibodies are also ideal for veterinary research and reactive with ovine, porcine, caprine, feline, canine, bovine and equine protein targets. Santa Cruz Biotechnology primary monoclonal antibodies are suitable for laboratory research applications and are not intended for diagnostic or therapeutic use.

SDHA Antibody (F-2) Specifications

Alternate Product Name: Succinate Dehydrogenase

Product Clone Name: F-2

Classification: Primary Antibody

Clonality: Monoclonal Antibody

Antibody Isotype: IgG_2a kappa light chain

Amino Acids: 637-664

Epitope Target: SDHA

Epitope Species: human

Product Reactivity/Detection: precursor and mature SDHA

Species Reactivity/Detection: mouse, rat, human

Additional Mammalian Reactivity: porcine

Additional Non-Mammalian Reactivity: N/A

Applications: WB, IP, IF, IHC(P), ELISA

Vialing Information: Each vial contains 200 µg IgG_2a kappa light chain in 1.0 ml of PBS with < 0.1% sodium azide and 0.1% gelatin

Research Citations: 17 citations in various scientific publications

Available Conjugates/Forms for SDHA Antibody (F-2): AC: Agarose, AF488: Alexa Fluor^® 488, AF546: Alexa Fluor^® 546, AF594: Alexa Fluor^® 594, AF647: Alexa Fluor^® 647, AF680: Alexa Fluor^® 680, AF790: Alexa Fluor^® 790, FITC: Fluoroscein, HRP: Horseradish Peroxidase, P: Blocking Peptide, PE: Phycoerythrin