SCO2 CRISPR/Cas9 KO Plasmid (h): sc-402686
- Target species: human
- 20 µg of transfection-ready, purified plasmid DNA; Suitable for up to 20 transfections
- SCO2 CRISPR/Cas9 Knockout (KO) Plasmid (h) is a pool of plasmids, each encoding Cas9 nuclease and a target-specific 20 nt guide RNA (gRNA) designed for maximum knockout efficiency using sequences derived from the GeCKO v2 library
- gRNA sequences direct Cas9 to induce site-specific double-strand breaks (DSBs) in the SCO2 genomic locus, resulting in gene knockout through non-homologous end joining (NHEJ)
- The puromycin resistance and RFP genes are flanked by LoxP sites, enabling removal of selection markers via Cre recombinase (Cre Vector: sc-418923) after establishing stable knockout cell lines
Ordering Information
| Product Name | Catalog # | UNIT | Price | Qty | FAVORITES | |
SCO2 CRISPR/Cas9 KO Plasmid (h) | sc-402686 | 20 µg | $397.00 |
Overview
SCO2 encodes a mitochondrial inner membrane-associated metallochaperone required for cytochrome c oxidase (complex IV) biogenesis by delivering copper to the COX2 subunit and supporting assembly of the respiratory chain. Through its role in oxidative phosphorylation, SCO2 helps maintain mitochondrial membrane potential, ATP production, and redox homeostasis, linking it to cellular energy metabolism and stress responses. Disruption of SCO2 impairs complex IV activity and can shift cells toward altered metabolic states, impacting processes such as reactive oxygen species handling and apoptosis sensitivity. Pathogenic variants in human SCO2 are associated with severe mitochondrial encephalocardiomyopathy phenotypes and cardiomyopathy, making it a relevant target for studying mitochondrial disease mechanisms.
SCO2 CRISPR/Cas9 KO Plasmid (h) is a pool of plasmids designed for targeted disruption of the SCO2 gene in human cell lines. Each plasmid co-expresses a unique single guide RNA (sgRNA) targeting a distinct site within the SCO2 together with the Streptococcus pyogenes Cas9 nuclease. The plasmids also encode GFP, allowing fluorescent identification and enrichment of successfully transfected cells by fluorescence microscopy or flow cytometry.
The multi-guide design increases the likelihood of generating insertions or deletions (indels) that disrupt the SCO2 open reading frame following Cas9-mediated double-strand break formation. DNA breaks introduced by the CRISPR/Cas9 system are repaired through endogenous non-homologous end joining (NHEJ) pathways, frequently resulting in frameshift mutations that abolish SCO2 protein expression.
This CRISPR knockout system enables efficient generation of SCO2-deficient cell models for investigation of SCO2 signaling, functional genomics studies, cancer biology research, and evaluation of therapeutic responses in human cell lines.
Key Features
- sgRNAs targeting SCO2 exon(s) critical for SCO2 function
- Co-expression of SpCas9 and sgRNA from a single plasmid for simplified delivery
- GFP reporter for identification of transfected cells
- Pool of plasmids targeting multiple SCO2 genomic sites to improve knockout efficiency
- Compatible with delivery by transfection
Design Variants
CRISPRs +/- HDRs
- gRNAs encoded by SCO2 CRISPR/Cas9 KO Plasmid (h) and SCO2 CRISPR/Cas9 KO Plasmid (h2) target distinct sites within the SCO2 locus. One or both targeting designs may be available. See Related Products for availability.
- HDR donor constructs encoded by SCO2 HDR Plasmid (h) and SCO2 HDR Plasmid (h2) contain a puromycin resistance cassette and an RFP reporter flanked by SCO2 homology arms to support homology-directed repair at defined SCO2 target sites corresponding to the CRISPR/Cas9 KO designs. HDR donor availability may vary. See Related Products for availability.
For Research Use Only. Not Intended for Diagnostic or Therapeutic Use.